Pleasanton site for large-scale oligonucleotide manufacturing

With over 25 years of experience, Thermo Fisher Scientific is dedicated to making quality and custom DNA and RNA oligos for your application. We are proud to offer large-scale oligo synthesis services to help ensure our customers obtain desired customized oligos in bulk quantities. Whether for scale-up, manufacturing, or OEM, Thermo Fisher Scientific is here to support your large-scale oligo synthesis needs.

Custom oligo service request

Thermo Fisher Scientific's mission is to enable our customers to make the world healthier, cleaner, and safer in industries such as:

  • Life science tools and reagents for basic and biomedical research
  • In vitro diagnostics (IVD) and molecular diagnostics (MDx)
  • Therapeutics


Large-scale oligo synthesis

Whether you're looking for milligram, gram, or kilogram amounts, Thermo Fisher Scientific can support you with large-scale oligo synthesis services. We offer a complete range of custom-synthesized oligonucleotides built to your specifications. To customize oligos for stability and function choose from a variety of 5', 3', and internal modifications. Table 1 lists some of the modifications available for large-scale oligo synthesis. Oligos can be ordered desalted or purified via cartridge, HPLC, or PAGE methods to match the oligo design and your downstream application.

Explore the evolving world of large-scale oligo synthesis in our on-demand webinar, where we delve into modern techniques, technologies, and emerging applications from R&D to clinical diagnostics and therapeutics. Watch and gain insights into the challenges associated with scaling up synthesis and learn how Thermo Fisher Scientific can support you with customized support throughout the development journey.

Table 1. Large-scale oligo synthesis modification types

Modification typePurpose in oligo designExamplesApplications
Attachment chemistry or linkersLinking oligo to another molecule or surfaceAmino C6, C12
Biotin C6, TEG
Thiol C3, C6
  • Affinity labels
  • Fluorescent tags
  • Nanoparticle conjugation
PhosphorylationFor oligos used as a substrate for DNA ligasePhosphate 3’ or 5’
  • DNA ligase
  • Block DNA polymerase extension
Phosphorothioate bondsIncrease resistance to nuclease degradationF, O, E, Z: IUPAC codes for phosphorothioated A, C, G, T
  • Therapeutic research
  • Antisense research
SpacersPhysically create space between a moiety and hybridizing region of the oligoC3 spacer, PC spacer
  • Attachment of fluorophores
  • Block DNA polymerase extension
Modified basesAid in cross-linking and nuclease resistanceLNA, L-DNA
  • Therapeutic research
Fluorophores and quenchersRe-emit/absorb light and emit heatAlexa Fluor, Cy3, FAM, and Rhodamine Red dyes
  • Antisense research
  • qPCR and detection assays


OEM oligos

To manufacture products according to your specifications, leverage our industry-leading expertise and infrastructure as a comprehensive third-party OEM service. We provide kitting, labeling, and commercialization services for oligos. As a company providing thousands of products to customers globally, Thermo Fisher Scientific has developed expertise in supply chain management, product manufacturing, and order fulfillment. As a vertically integrated organization, we control reagent quality throughout the nucleic acid synthesis process which reduces batch-to-batch variability of oligos.

To inquire about OEM oligos: Contact us
Learn more: OEM & commercial supply services


Certifications for large-scale oligo synthesis

Thermo Fisher Scientific’s oligo manufacturing suite in Pleasanton, CA is powered by more than 100 years of combined experience. We offer dedicated ordering and fulfillment services that are integrated into your organization's systems and processes. Our capabilities extend to the production of oligonucleotide components for in vitro diagnostics (IVDs) and analyte-specific reagents (ASRs).

Our oligo laboratories operate under ISO 9001 and ISO 13485 certifications and are maintained under our quality management systems. We use a scalable quality approach that is customized to your needs and requirements and provides traceability and good documentation practices, including certificates of analysis, to help ensure consistent quality and safety downstream.

  • ISO 13485:2016
    The design, research, development, manufacture, distribution, and servicing of consumables, kits, assays, and oligo related products for life sciences research and in vitro diagnostic purposes. (BSI-FM561905)
  • ISO 9001:2015
    The design, development (R&D), manufacture, distribution, and servicing of consumables for life science research using our assay and oligo related products. (BSI-FM534534)


Commercial oligo manufacturing process*

Proposal
  1. Sign CDA
  2. Assign Project Manager
  3. Gather requirement
  4. Assess gaps
  5. Outline project
  6. Supply agreement
Development supportProcess development
  1. Define strategies
  2. Optimize product
  3. Design packaging
  1. Develop process
  2. Validate process
  3. Manufacturing pilot lots
Custom manufacturingSupportDistribution
  1. Plan capacity
  2. In-source material
  3. Kitting, labeling
  4. Optimize process
  1. QC and compliance audits
  2. Business reviews
  1. Supply chain management
  2. Relationship management
Step 1 : Proposal
Proposal
  1. Sign CDA
  2. Assign Project Manager
  3. Gather requirement
  4. Assess gaps
  5. Outline project
  6. Supply agreement
Step 2 : Development
Development supportProcess development
  1. Define strategies
  2. Optimize product
  3. Design packaging
  1. Develop process
  2. Validate process
  3. Manufacturing pilot lots
Step 3 : Commercialization
Custom manufacturingSupportDistribution
  1. Plan capacity
  2. In-source material
  3. Kitting, labeling
  4. Optimize process
  1. QC and compliance audits
  2. Business reviews
  1. Supply chain management
  2. Relationship management

* Process may be modified on a project-by-project basis.

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Resources and support

For Research Use Only. Not for use in diagnostic procedures.