Gaussia Luciferase Assays & Vectors

We offer luciferase reporter reagents, multiple cloning site vectors, and flash and dual-spectral luciferase assay kits based on the naturally secreted Gaussia luciferase. Gaussia luciferase is a 20kDa protein from the marine copepod, Gaussia princeps. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output generated by the luciferase reaction can be correlated to the amount of Gaussia luciferase protein produced and used to determine the activity of the promoter driving Gaussia expression. The signal produced by Gaussia luciferase shows strong flash kinetics and is considerably greater than flash signals from either firefly or Renilla luciferases assayed under similar conditions.

We offer luciferase reporter reagents, multiple cloning site vectors, and flash and dual-spectral luciferase assay kits based on the naturally secreted Gaussia luciferase. The assay kit is optimized for use with our Gaussia Luc Vectors but is fully compatible with other Gaussia luciferases and derivatives that use coelenterazine as a substrate.

The Gaussia Dura gene used in the Thermo Scientific Gaussia Dura Luc Vectors is derived from the wild type Gaussia gene and provides equally bright but more stable light output. The Gaussia Glow Assay Kit reagent yields a Gaussia Dura luciferase reaction with glow-type kinetics and is recommended for luminometers without injectors or for applications requiring batch processing of samples.

Oxidative decarboxylation of coelenterazine by Gaussia luciferase produces coelenteramide, carbon dioxide, and light.