Protein Expression Resources

Resources to help you optimize your protein expression

The protein expression resources center is here for you, whether you want to review the basics, gain more in-depth knowledge, or discover our latest research tools. This resource hub contains rich and reliable technical content designed for new and experienced expression scientists.

 

Discover our latest protein expression posters to drive workflow efficiencies

Biologics expression

Recombinant protein expression is one of the most powerful techniques used in biologics development. Below we present solutions to help optimize your biologics discovery expression and accelerate your path to clinic.

Optimizing the therapeutic antibody production workflow

Learn about the tips and technologies that can be applied to help maximize the success of your therapeutic antibody production workflow.

Membrane protein expression

Producing membrane proteins can be difficult as it can be hard to preserve the correct conformation of the protein in isolation from its native environment. we present some protein expression tools that can significantly enhance the ability of researchers to study membrane protein biology.

Optimized expression of membrane protein in the Expi293 and ExpiCHO Expression Systems

In this poster, we present case studies on the optimization of ion channel and GPCR expression using ExpiCHO and Expi293 expression systems. Additionally, we demonstrate the utility of GNTI-, Inducible and Inducible/GNTI- Expi293 cell lines) to allow for regulated expression and/or glycosylation of membrane proteins.

Optimizing the membrane protein expression and purification workflow

Learn about the tips and technologies that can be applied to maximize the success of your membrane protein expression and purification workflow.

Viral protein expression

Rapid protein production is essential for infectious disease research. Below we present solutions to generate high-quality proteins for structural research, serological testing, therapeutics discovery and vaccine candidates screening.

Advancing the development of therapeutics against infectious diseases with optimized expression systems.

In this white paper, learn how our optimized protein expression systems can help understand viral pathogenicity and combat rapidly evolving pathogens.

Setting up an effective protein expression lab

Whether you are setting up a new lab or introducing protein expression into your current workflow, in this section you can find short- and long-term planning considerations and resources for workflow optimization.

Setting up an effective protein expression workflow

In this poster, we present resources and strategies to optimize the Cloning, Expression, Purification and Analysis (CEPA) workflow

Optimizing every step of your protein expression workflow

Our selection guide allows you to compare workflow solutions for each host system side-by-side, find the one that matches your field and timeline goals and assess the products necessary to enable a successful outcome.

Protein expression resources list

 

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Type Title
Protocol ExpiCHO essential protocol guide
Protocol Expi293 expression system user guide
Protocol ExpiSf smart start guide
Protocol Protein Expression Protocols
Protocol calculator Expi Systems Protocol Calculator
Video ExpiCHO: Transient CHO Expression Protocol
Video Expi293: Transient HEK 293 Expression Protocol
Video Expi Protein Expression System Starter Kit Tips and Tricks
Handbook Protein Research Handbook (2016)
Handbook Protein Expression Handbook (2015)
Literature Gibco Expi protein expression systems brochure (Expi293 and ExpiCHO)
Literature ExpiSf expression system flyer
Literature Expi293 tools for structural biology and inducible expression
Methods article The Pierce Protein Methods Library
Methods article GST-tagged Proteins–Production and Purification
Methods article His-tagged Proteins–Production and Purification
Methods article Luciferase Reporters
Methods article An Overview of Post-Translational Modification
Methods article Phosphorylation
Methods article Glycosylation
Methods article An Overview of Protein Expression Systems
Methods article Cell-Free Protein Expression
Selection guide Protein Expression Workflow Selection Guide
Application note Adaptation of the ExpiSf Expression System for high-level protein production with the WAVE bioreactor system
Application note ExpiCHO Expression System—Protocols for 24 and 96 deep well blocks and mini bioreactor tubes
Application note ExpiCHO Expression System: A guide to optimizing protein A purification of monoclonal antibodies
Application note Adaptation of Expi293 to the WAVE Bioreactor System
Application note Expi293 MembranePro Expression System App Note
Application note The Expi293 Expression System—Optimizing protein yield
Application note Expi293 Expression System—Optimizing culture conditions to maximize protein yield and enable use of 96-well microtiter plates
Application note Using the Expi293 Expression System under animal origin–free (AOF) conditions
Application note Product Application Notes
Application note Glycoprotein expression in a human IVT system. Expression of partially N-linked glycoproteins using the 1-Step Human Coupled in vitro Translation System.
Application note Expression of highly active proteins using a cell-free human IVT system. Active caspase-3 produced by cell-free protein expression with the 1-Step Human High-Yield In Vitro Translation Kit is more functional than recombinant caspase-3 expressed in bacteria.
Application note System for high-throughput, high-yield in vitro translation. HTP protein expression using 1-Step Human High-Yield IVT Kits and Pierce 96-well Microdialysis Plates.
Application note Choosing a vector and purification method for in vitro protein expression. Strategies for optimizing expression and purification of functional proteins produced using the Thermo Scientific 1-Step High Yield IVT protein expression system.
Application note Protocol for in vitro protein expression using mRNA templates. Use the 1-Step Human Coupled DNA IVT kit to efficiently translate mRNA templates.
Application note Simultaneous dual-emission detection of luciferase reporter assays. Performing dual-spectral luciferase assays using the BMG Labtech POLARstar Omega plate reader.
Application note Cell-free protein expression system for generating stable isotope-labeled proteins. Rapidly express protein standards for quantitative mass spectrometry.
Application note Highly sensitive multiplex luciferase reporter assays. Monitor changes in NFkB and CRE promoter activity using bioluminescent reporter assays.
Scientific poster Scalable protein and AAV production in the ExpiSf Expression System
Scientific poster ExpiCHO: Surpassing the Performance of Expi293 in a Transient CHO Expression System
Scientific poster Optimized expression of membrane proteins in the Expi293 and ExpiCHO expression systems: new tools for difficult to express proteins
Scientific poster Strategies for high-titer protein expression using the ExpiCHO and Expi293 transient expression systems
Scientific poster ExpiSf expression system: a chemically defined baculovirus-based system for enhanced protein and virus production in Sf9 cells 
Support center Protein Expression Support Center
Support center Adenoviral Gene Delivery for Mammalian Expression Support
Support center Algae Expression Support
Support center Bacterial Expression Support
Support center Basic Mammalian Expression Support
Support center Cell-Free Expression Support
Support center Insect Expression Support
Support center Lentiviral Gene Delivery for Mammalian Expression Support
Support center Mammalian Expression for High-Yield Protein Production Support
Support center Mammalian Expression for Membrane Protein Production Support
Support center Protein Expression Basics Support
Support center Reporter & Specialty Vectors for Mammalian Expression Support
Support center Yeast Expression Support
Webinar Enabling Next-Gen Targeted siRNA with ExpiCHO
Webinar Expression, purification and characterization in Expi293 and ExpiCHO
Webinar Factor V Expression in ExpiCHO Cells
Webinar Expi293: New Tools for structural Biology and difficult proteins
Webinar ExpiSf Expression System: A Chemically defined baculovirus-based expression system for enhanced protein and virus production in Sf9 cells
Webinar ExpressionWorld Webcasts

For Research Use Only. Not for use in diagnostic procedures.