Protein-Expression-Optimization

Nearly all molecular biology experiments start with cloned DNA. The gene can be a starting point for protein expression, structural analysis, or a functional experiment.

Researchers may derive their DNA from PCR using natural sources or purchase it from a premade clone collection. Buying the gene from an ORF collection is the fastest way if the gene of interest is available. However, you have to rely on preexisting material. Modifying these constructs can be time consuming and costly. Gene synthesis offers the opportunity to design every construct from scratch—tailored for the experiment. Your starting point can be any sequence information. This offers maximum flexibility to get the gene of choice in about 8–10 business days, 100% sequence verified, cloned into a vector of choice.

Synthesis of an optimized gene further enhances protein expression levels. In addition, optimized genes are resistant to RNA knock down.

Tools for optimizing protein expression

 100% sequence verified clone available in less than a weekProtein expression with no gene design constraintsImproved protein expression levels through gene optimization
 
 
 
 
Key featuresFastest way to obtain a sequence verified cloneFull service that delivers validated genes with no limitation in sequence designFull service that delivers validated genes with improved expression yields & no limitation in sequence design
Time to get to expression1 week6–15 days to receive clones ready for use6–15 days to receive clones ready for use
Gene size1up to 20 kbup to 20 kb
Number of fragments1N/AN/A
What is deliveredGlycerol stock of uORF entry clone; ORF card data available onlineSynthetic wild type gene; 5 µg lyophilized DNA cloned in GeneArt® standard vector; Quality Assurance Documentation including sequence verificationOptimized gene; 5 µg lyophilized DNA cloned in GeneArt® standard vector; Quality Assurance Documentation including sequence verification
Choice of expression vectorsAny Gateway® Destination vectorAny vectorAny vector
Improvement of expression yieldsN/AN/AUp to 100 fold
Free webtool for search & designYesYesYes
 Order NowConfigure your projectConfigure your project

For Research Use Only. Not for use in diagnostic procedures.