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The CellTrace Far Red kit is used to monitor distinct generations of proliferating cells by dye dilution. Live cells are covalently labeled with a very bright, stable dye. Every generation of cells appears as a different peak on a flow cytometry histogram.
CellTrace Far Red | |
---|---|
Excitation/Emission (in nm) | 630/661 |
Standard filter set | Alexa Fluor 647 |
Storage conditions | ≤–20°C |
Human T lymphocytes stained with CellTrace Far Red Cell Proliferation reagent and stimulated in culture for 5 days. The discrete peaks represent successive generations of live cells. The unstimulated parent generation is indicated in purple. Analysis was completed using an Attune Acoustic Focusing Cytometer with 638 nm excitation and a 660/20 nm bandpass emission filter.
For Research Use Only. Not for use in diagnostic procedures.