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Porcine Respiratory Diseases
Having the right diagnostic tools is crucial to help you investigate pig infections
Swine respiratory diseases have a significant impact around the world and are responsible for proven important productivity losses in pig herds. Often, multiple infectious agents are involved: virus, bacteria, mycoplasma, and parasites are all potential causes. These infectious agents act together to increase the severity and duration of the disease. Farmers, veterinarians, and veterinary diagnostic labs have to work together to identify the agents involved. Regularly submitting samples to a diagnostic laboratory will help farmers and veterinarians keep up to date with what’s going on in an operation or herd.
ELISA tests and real-time PCR tests provide complementary information about an infection. A combination of ELISA tests (for antibodies detection) and real-time PCR tests (for pathogen detection), when used at the right time in the disease's progression, can give veterinarians and farmers a more complete picture of the infection. This can help them detect disease earlier and make management decisions much faster on the farm to stop the spread of disease and, ultimately, save money.
Choose your pathogen of interest and explore the solutions Thermo Fisher Scientific has to offer
In some countries, depending on the prevalence of diseases, other pathogens can be involved in swine respiratory diseases, such as classical swine fever virus or pseudorabies virus.
Porcine Reproductive and Respiratory Syndrome virus—PRRSv
PRRS is a highly contagious disease caused by a virus belonging to the Arterivirus family. The PRRS virus is a small (50–60 nm), enveloped RNA virus with at least two different membrane proteins at its surface. The virus causes respiratory and influenza-like symptoms as well as fertility problems, abortion, and the birth of runts.
PRRS is one of the most economically damaging diseases for the pig industry and is spread throughout the world, affecting domesticated pigs in particular.
Thermo Fisher Scientific develops unique diagnostic schemes for PRRSV, with sampling, sample preparation tools, and workflows for real-time PCR and ELISAs.
* Terms and conditions apply. Please see offer details web pages for more information.
Regulatory requirements vary by country; products may not be available in your geographic area.
Enzootic pneumonia—Mycoplasma hyopneumoniae (M. hyo.)
Enzootic pneumoniae, caused by Mycoplasma hyopneumoniae, occurs worldwide and causes a chronic infectious pneumonia in pigs that is characterized by a persistent dry cough, decreased growth rate, and sporadic respiratory distress.
VetMAX M. hyopneumoniae real-time PCR reagents and controls allow rapid screening for the presence of Mycoplasma hyopneumoniae. M. hyo. can be a significant economic burden, particularly when swine influenza virus (SIV) or porcine reproductive and respiratory syndrome (PRRS) are endemic.
Regulatory requirements vary by country; products may not be available in your geographic area.
Swine Influenza Virus—SIV
Influenza A is a zoonosis that poses a threat to human and animal health worldwide. SIV is a highly contagious viral infection in pigs caused by a variety of closely related influenza A viruses. These viruses are noted for their ability to create new strains such as H1 N1, H1 N2, and H3 N2. An outbreak is typically preceded by 1–3 individual cases and then spreads rapidly within a herd, mainly by aerosolization (coughing, nasal discharge) and pig-to-pig contact. Accurately monitoring and detecting exposure is critical in preventing its spread.
Real-time PCR screening and subtyping kits are used for the rapid detection of swine influenza virus (SIV). VetMAX-Gold SIV solutions are rapid, easy-to-use real-time PCR screening and subtyping kits that detectinfluenza virus in pigs with superior sensitivity, specificity, repeatability, and reproducibility.
The PrioCHECK Swine Influenza Ab Serum Plate Kit is an indirect ELISA used for the detection of antibodies against swine type A Influenza, detecting all H and N subtypes. This kit primarily recognizes antibodies to matrix and nucleocapsid proteins specific to Influenza type A. It’s principally used for control rather than diagnosis; serological screening, decrease of MDA for vaccination, and control of vaccination.
Regulatory requirements vary by country; products may not be available in your geographic area.
Porcine Circovirus type 2 - PCV2
PCV2 is a DNA virus classified in the Circovirus genus of the Circoviridae family. This virus is now recognized as the main infectious agent involved in the development of postweaning multisystemic wasting syndrome (PMWS). PCV2 is mainly excreted in the feces and urine, but is also found in bronchial and nasal secretions. Its transmission is mainly horizontal through healthy carriers and sick animals. PMWS is found throughout the world (in many countries in Europe, North America, and Asia) and causes heavy economic losses due to its impact on breeding performance.
The VetMAX Porcine PCV2 quantitative real-time PCR kit enables you to detect and quantify the presence of PCV2 virus in the sample, and the presence of the internal endogenous positive control (IPC), which validates the purification of the sample and avoids false negative results.
Regulatory requirements vary by country; products may not be available in your geographic area.
Porcine Reproductive and Respiratory Syndrome virus—PRRSv
PRRS is a highly contagious disease caused by a virus belonging to the Arterivirus family. The PRRS virus is a small (50–60 nm), enveloped RNA virus with at least two different membrane proteins at its surface. The virus causes respiratory and influenza-like symptoms as well as fertility problems, abortion, and the birth of runts.
PRRS is one of the most economically damaging diseases for the pig industry and is spread throughout the world, affecting domesticated pigs in particular.
Thermo Fisher Scientific develops unique diagnostic schemes for PRRSV, with sampling, sample preparation tools, and workflows for real-time PCR and ELISAs.
* Terms and conditions apply. Please see offer details web pages for more information.
Regulatory requirements vary by country; products may not be available in your geographic area.
Enzootic pneumonia—Mycoplasma hyopneumoniae (M. hyo.)
Enzootic pneumoniae, caused by Mycoplasma hyopneumoniae, occurs worldwide and causes a chronic infectious pneumonia in pigs that is characterized by a persistent dry cough, decreased growth rate, and sporadic respiratory distress.
VetMAX M. hyopneumoniae real-time PCR reagents and controls allow rapid screening for the presence of Mycoplasma hyopneumoniae. M. hyo. can be a significant economic burden, particularly when swine influenza virus (SIV) or porcine reproductive and respiratory syndrome (PRRS) are endemic.
Regulatory requirements vary by country; products may not be available in your geographic area.
Swine Influenza Virus—SIV
Influenza A is a zoonosis that poses a threat to human and animal health worldwide. SIV is a highly contagious viral infection in pigs caused by a variety of closely related influenza A viruses. These viruses are noted for their ability to create new strains such as H1 N1, H1 N2, and H3 N2. An outbreak is typically preceded by 1–3 individual cases and then spreads rapidly within a herd, mainly by aerosolization (coughing, nasal discharge) and pig-to-pig contact. Accurately monitoring and detecting exposure is critical in preventing its spread.
Real-time PCR screening and subtyping kits are used for the rapid detection of swine influenza virus (SIV). VetMAX-Gold SIV solutions are rapid, easy-to-use real-time PCR screening and subtyping kits that detectinfluenza virus in pigs with superior sensitivity, specificity, repeatability, and reproducibility.
The PrioCHECK Swine Influenza Ab Serum Plate Kit is an indirect ELISA used for the detection of antibodies against swine type A Influenza, detecting all H and N subtypes. This kit primarily recognizes antibodies to matrix and nucleocapsid proteins specific to Influenza type A. It’s principally used for control rather than diagnosis; serological screening, decrease of MDA for vaccination, and control of vaccination.
Regulatory requirements vary by country; products may not be available in your geographic area.
Porcine Circovirus type 2 - PCV2
PCV2 is a DNA virus classified in the Circovirus genus of the Circoviridae family. This virus is now recognized as the main infectious agent involved in the development of postweaning multisystemic wasting syndrome (PMWS). PCV2 is mainly excreted in the feces and urine, but is also found in bronchial and nasal secretions. Its transmission is mainly horizontal through healthy carriers and sick animals. PMWS is found throughout the world (in many countries in Europe, North America, and Asia) and causes heavy economic losses due to its impact on breeding performance.
The VetMAX Porcine PCV2 quantitative real-time PCR kit enables you to detect and quantify the presence of PCV2 virus in the sample, and the presence of the internal endogenous positive control (IPC), which validates the purification of the sample and avoids false negative results.
Regulatory requirements vary by country; products may not be available in your geographic area.
Optimized workflows for serology and molecular diagnostics from sampling to result
At Thermo Fisher Scientific we work closely with our customers to develop and implement reliable diagnostic test solutions. Our comprehensive portfolio of diagnostics provides a wide range of sample collection tools, sample preparation kits and instruments, and ELISA and real-time PCR assays and instruments.
Sampling
Whether it's a blood/serum sample, or a tissue, organ, semen, or oral fluid sample collected with a GenoTube Livestock Swab, our goal is to help you process compatible workflow with your existing laboratory process.
- The GenoTube Livestock Swab is designed to quickly dry and stabilize fluid samples. It’s validated for oral fluids and blood samples and optimized sample extraction protocols for real-time PCR and ELISA
Sample preparation
The MagMAX sample preparation system helps enable efficient, reliable, and easy nucleic acids purification from pig samples. MagMAX nucleic acid isolation kits, along with KingFisher magnetic particle processors, assist the diagnostic laboratory in obtaining the best possible PCR results from samples.
The new MagMAX CORE workflow offers:
- Faster instrument processing times, allowing you to determine diagnostic results more quickly
- Premixed washes that don’t require the addition of alcohols
- Higher-quality nucleic acids, which may lead to less retesting and fewer workflow interruptions
See all our solutions on the sample preparation solutions web page ›
Pathogen detection with real-time PCR
Real-time PCR is the most sensitive method to provide clear proof of pathogen infection or non-infection.
- PCR (Polymerase Chain Reaction) is a diagnostic test designed to confirm an individual pig’s clinical disease, a current infection, or to diagnose the cause of death in pigs
- PCR is especially valuable in the early stages of an infection when antibodies have not yet been built
- PCR provides an immediate answer to the current infection
In cases where a positive result is found, the nucleic acid can be used for sequencing.
VetMAX real-time PCR solutions and Applied Biosystems real-time PCR instruments help ensure that the qPCR test results you deliver to your customers are consistently rapid, accurate, and actionable.
Antibodies detection with ELISA
ELISA is used to monitor pig herd health by screening for changes in antibody status.
- If a specific pathogen’s antibodies are found in the blood, it means the infection occurred sometime in the past.
- It’s an easy-to-use detection method for both large and small laboratories.
PrioCHECK ELISA kits and software have been developed to make every step of your ELISA workflow easier and more efficient. We offer a wide selection of centrifuges, cold storage equipment, dispensers, incubator/shakers, microplates, pipettes and tips, and microplate readers and washers to meet all your ELISA needs.
Sampling
Whether it's a blood/serum sample, or a tissue, organ, semen, or oral fluid sample collected with a GenoTube Livestock Swab, our goal is to help you process compatible workflow with your existing laboratory process.
- The GenoTube Livestock Swab is designed to quickly dry and stabilize fluid samples. It’s validated for oral fluids and blood samples and optimized sample extraction protocols for real-time PCR and ELISA
Sample preparation
The MagMAX sample preparation system helps enable efficient, reliable, and easy nucleic acids purification from pig samples. MagMAX nucleic acid isolation kits, along with KingFisher magnetic particle processors, assist the diagnostic laboratory in obtaining the best possible PCR results from samples.
The new MagMAX CORE workflow offers:
- Faster instrument processing times, allowing you to determine diagnostic results more quickly
- Premixed washes that don’t require the addition of alcohols
- Higher-quality nucleic acids, which may lead to less retesting and fewer workflow interruptions
See all our solutions on the sample preparation solutions web page ›
Pathogen detection with real-time PCR
Real-time PCR is the most sensitive method to provide clear proof of pathogen infection or non-infection.
- PCR (Polymerase Chain Reaction) is a diagnostic test designed to confirm an individual pig’s clinical disease, a current infection, or to diagnose the cause of death in pigs
- PCR is especially valuable in the early stages of an infection when antibodies have not yet been built
- PCR provides an immediate answer to the current infection
In cases where a positive result is found, the nucleic acid can be used for sequencing.
VetMAX real-time PCR solutions and Applied Biosystems real-time PCR instruments help ensure that the qPCR test results you deliver to your customers are consistently rapid, accurate, and actionable.
Antibodies detection with ELISA
ELISA is used to monitor pig herd health by screening for changes in antibody status.
- If a specific pathogen’s antibodies are found in the blood, it means the infection occurred sometime in the past.
- It’s an easy-to-use detection method for both large and small laboratories.
PrioCHECK ELISA kits and software have been developed to make every step of your ELISA workflow easier and more efficient. We offer a wide selection of centrifuges, cold storage equipment, dispensers, incubator/shakers, microplates, pipettes and tips, and microplate readers and washers to meet all your ELISA needs.
Resources
ELISA or PCR? Not all tests are created equal
Read article ›
Fast, reliable, and high-quality diagnostic solutions for SIV, PRRSV, PCV2, and M. hyo
Download scientific poster ›
Development and validation of a complete workflow solution for SIV testing
Download scientific poster ›
Influenza A Virus Detection from Oral Fluid and Nasal Swabs in IAV Inoculated Pigs
Download scientific poster ›
Earlier and easier diagnostic tools for PRRSV herd management:
Comparison of sampling and prevalence under field conditions
Download scientific poster ›
Opportunities and limitations of Mycoplasma hyopneumoniae PCR testing in oral fluids to confirm involvement in respiratory disease.
Download scientific poster ›
Detection of respiratory pathogens in oral fluid; sampling recommendations in commercial conditions:
Download presentation ›
Using Diagnostics to Evaluate Porcine Respiratory Coronavirus Type 2 (PCV2)
See video ›
Dr. Daniel Linhares on Diagnostics, Swine Mycoplasma hyopneumoniae
See video ›
Dr. Lisa Becton Talks about Diagnostics Role in PRRS
See video ›
Dr. Lisa Becton—What’s the Role of Diagnostics with SIV?
See video ›
Diagnosis of PRRS virus infection using oral fluids
Discover all our videos on swine with experts interviews ›
Download scientific poster ›
See the video ›
For Veterinary Use Only. For In Vitro Use Only. Regulatory requirements vary by country; products may not be available in your geographic area.
For Veterinary Use Only. For In Vitro Use Only. Regulatory requirements vary by country; products may not be available in your geographic area.