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Enhanced performance of low-flow LC-MS and HPLC separations

Low-flow chromatography is ideal when detailed sample information is required from small sample volumes, such as in omics research. Compared with analytical flow (>100 µL/min), low-flow LC-MS (<100 µL/min) typically provides a large increase in sensitivity. Low-flow columns—also known as nano, capillary, or micro columns—have a smaller diameter compared to analytical columns.

Thermo Scientific nano-, capillary-, and micro-flow columns offer excellent sensitivity and resolution in easy-to-use formats:

  • Thermo Scientific EASY-Spray HPLC Columns
  • Thermo Scientific single and double nanoViper UHPLC Columns
  • Thermo Scientific μPAC Neo HPLC Columns

µPAC HPLC column technology is a silicon revolution in chromatography

Our patented technology overcomes the physical limitations of packed bed LC columns. It reduces peak dispersion which leads to better separation. Furthermore, this unique technology increase data reproducibility and decrease costs as these columns are highly robust and reliable.

 

μPAC Neo High Throughput Columns for single-cell to comprehensive proteomic applications

Improve your chromatography and analyze hundreds of samples per day, day after day, and do it without sacrificing performance and reproducibility.

 

Low-flow HPLC columns powered with technology that's beyond brilliant

Learn more about the power of Thermo Scientific low-flow HPLC columns and discover column designs that ensure near zero-dead volume connections to give you confidence in your data quality.

 

Low-flow columns for reliable and robust data

Low-flow (U)HPLC column formats

EASY-Spray (U)HPLC ColumnsSingle and Double nanoViper UHPLC ColumnsµPAC Neo HPLC Columns
EASY-Spray HPLC Columns
Double nanoViper HPLC Columns
  • Click-and-spray connection with EASY-Spray MS source
  • nanoViper connection
  • Integrated column and emitter
  • Integrated temperature control
  • For use with Thermo Scientific MS systems
  • Universal Thermo Scientific nanoViper fingertight fittings for the column inlet and/or outlet
  • nanoViper connection
  • Separate emitters
  • Compatible with all low-flow U/HPLC instruments
  • Excellent retention time stability
  • Low back pressure separations, improving column and emitter robustness
  • High flexibility
  • Compatible with all low-flow U/HPLC instruments
  • nanoViper connection
EASY-Spray columns
Choose an EASY-Spray column when:What makes an EASY-Spray column special?
  • You want simple connections with an EASY-Spray source. This is ideal for novice users.
  • Sample amount is limited
  • Analytical UHPLC does not provide sufficient sensitivity
  • Workflow simplicity is key
  • High sensitivity is required to identify proteins and peptides at low expression levels
  • Analyses are done in a targeted and untargeted way for screening and verification

Unique design provides uncompromised performance in an easy-to-use format for nano and capillary LC-MS analysis.
Features for optimum data quality:

  • Click and Spray connection to Thermo Scientific LC and MS instruments
  • Precision machined and positioned glass emitters
  • Integrated nanoViper zero-dead-volume (ZDV) unions
  • Integrated temperature control

 

Recommended EASY-Spray UHPLC columns by application

EASY-Spray UHPLC columns for bottom-up proteomics

EASY-Spray PepMap Neo UHPLC columns

The Thermo Scientific EASY-Spray PepMap Neo UHPLC columns are perfect for bottom-up proteomics. Packed at higher pressure and rated to 1500 bar, they provide consistent column-to-column performance, long column lifetime, and excellent efficiency. These benefits are true at any pressure.

Catalog numberProduct lineParticle size (µm)Column ID (µm)Column length (mm)Order
ES75150PNPepMap Neo columns275150Order now ›
ES75500PNPepMap Neo columns275500Order now ›
ES75750PNPepMap Neo columns275750Order now ›

Also see: EASY-Spray PepMap HPLC columns

EASY-Spray columns for top-down proteomics

EASY-Spray MAbPac RP columns

The Thermo Scientific MAbPac RP capillary column is best suited for the characterization of intact proteins in top-down proteomics, clinical and anti-doping applications where sample amount is limited, or sensitivity is crucial.

Catalog numberProduct lineParticle size (µm)Column length (mm)Column ID (µm)Order
ES907MAbPac RP column4150150Order now ›
Traps and accessories

Traps and accessories

For the best performance from your EASY-Spray column, consider investing in the following accessories.

Traps

Catalog numberProduct lineParticle size (µm)Column ID (µm)Media bed length (mm)Trap length (mm)Union typeOrder
174500PepMap Neo Trap Cartridge 3PK53005N/AN/AOrder now ›
164199PepMap nanotrap 500 bar 2PK510020150Nut/sleeveOrder now ›
164750PepMap nanotrap 500 bar 2PK510020150Double nanoViperOrder now ›
164535PepMap nanotrap 500 bar 2PK37520150Double nanoViperOrder now ›
164946PepMap nanotrap 1200 bar 2PK3752070Double nanoViperOrder now ›
164213PepMap nanotrap 500 bar 2PK520020150Nut/sleeveOrder now ›

PEEK tubing and trap holder

Catalog numberProduct lineFor use withOrder
174501PEEK with nanoViper fittings 30 µm X100 mm 2PK 1500 barLow-flow PepMap columnsOrder now ›
174502Trap holder + nanoViper fittings kit 1500 barLow-flow PepMap columnsOrder now ›
Choose these columns when:What makes these columns special?
  • Maximum flexibility is required
  • Changing the emitter and column independently is important

These stand-alone nano-, capillary, and micro-flow columns are:

  • Designed with single nanoViper and double nanoViper fingertight fittings for trouble-free connection
  • For robust separation in proteomics research, drug discovery, and high-throughput proteomics laboratories

Recommended nano- and capillary HPLC columns by application

Nano-/Capillary UHPLC columns for bottom-up proteomics

Double nanoViper PepMap Neo UHPLC columns

Use these columns for peptide bottom-up proteomics studies. They are packed at higher pressure and rated a 1500 bar and provide consistent column-to-column performance, long column lifetime, and excellent efficiency. These benefits are true at any pressure.

Catalog numberProduct lineParticle size (µm)Column ID (µm)Column length (mm)Order
DNV75150PNPepMap Neo column275150Order now ›
DNV75500PNPepMap Neo column275500Order now ›
DNV75750PNPepMap Neo column275750Order now ›
DNV150150PNPepMap Neo column
2150150Order now ›
SNV75750PNPepMap Neo column
275750Order now ›
SNV150150PNPepMap Neo column
2150150Order now ›

Also available: Single nanoViper PepMap C18 HPLC columns

Nano-/Capillary UHPLC columns for top-down proteomics

MAbPac capillary reversed phase (RP) HPLC column

The Thermo Scientific MAbPac RP capillary column is best suited for the characterization of intact proteins in top-down proteomics, clinical, and anti-doping applications where sample amount is limited, or sensitivity is crucial.

Catalog numberProduct lineParticle size (µm)Column ID (µm)Column length (mm)Order
164947MAbPac RP column4150150Order now ›
Nano-/Capillary UHPLC columns for free glycans

Accucore 150 Amide HILIC HPLC column

  • Separate hydrophilic biomolecules in HILIC mode
  • Excellent for released glycan separations
Catalog numberProduct lineParticle size (µm)Column ID (µm)Column length (mm)Order
16726-157569Amide HILIC column2.675150Order now ›
LC-MS connection accessories and emitters

These emitters, nanoViper tubing kits, and unions offer easy connection from your LC system to an EASY-Spray source.

Catalog numberProduct lineFor use withOrder
6040.2304Two Viper unionsSingle and Double nanoViper columnsOrder now ›
6041.5260nanoViper tubing 20 µm x 550 mmSingle and Double nanoViper columnsOrder now ›
ES993Emitter: 10 µm I.D.Single and Double nanoViper columnsOrder now ›
ES994Emitter: 15 µm I.DSingle and Double nanoViper columnsOrder now ›
Traps and accessories

For the best performance from your double nanoViper column consider investing in the following accessories.

Catalog numberProduct lineParticle size (µm)Column ID (µm)Media bed length (mm)Trap length (mm)Union typeOrder
174500PepMap Neo Trap Cartridge 3PK53005N/AN/AOrder now ›
164199PepMap nanotrap 500 bar 2PK510020150Nut/sleeveOrder now ›
164750PepMap nanotrap 500 bar 2PK510020150Double nanoViperOrder now ›
164535PepMap nanotrap 500 bar 2PK37520150Double nanoViperOrder now ›
164946PepMap nanotrap 1200 bar 2PK3752070Double nanoViperOrder now ›
164213PepMap nanotrap 500 bar 2PK520020150Nut/sleeveOrder now ›
Catalog numberProduct lineFor use withOrder
174501PEEK with nanoViper fittings 30 µm X100 mm 2PK 1500 barLow-flow PepMap Neo columnsOrder now ›
174502Trap holder + nanoViper fittings kit 1500 barLow-flow PepMap Neo columnsOrder now ›
Choose a μPAC Neo HPLC column when:What makes µPAC Neo HPLC columns special?
  • Highest resolution and peak capacities is required
  • Your samples span a wide concentration range
  • Highest LC-MS sensitivity is needed
  • You want to speed up your runtimes
  • LC-MS robustness is needed
  • You want an increased column lifetime
  • You prefer working at much lower back pressures than with packed-bed columns
  • When it is important to compare results from experiments spanning over time or geographical location
The unique separation path provides:
  • µ-pillar stationary backbone, micromachined in a silicon wafer
  • Flow path designed for highest analyte concentration during elution
  • Extra-high resolution separations, using up to 110 cm column lengths
  • Unique combination of performance and reliability for high sample coverage every time
μPAC Neo HPLC columns

These HPLC columns are ideally suited for bottom-up proteomics where resolution is critical to the success of the analysis. Our µPAC Neo columns provide excellent column-to-column reproducibility and peak capacity at a wide range of flow rates and sample amounts.

Catalog numberProduct lineColumn length (cm)Flow rate range (nL/min)Sample loadingGradient timeOrder
COL-NANO050NEOBµPAC Neo HPLC Column50100-75010-500 ng15 – 60 minOrder now ›
COL-NANO110NEOBµPAC Neo HPLC Column110100-750500-2000 ng90-150 minOrder now ›
COL-LOLO050NEOBLow-Load µPAC Neo HPLC Column50100-7500.1-1 ng15 – 60 minOrder now ›
COL-CAPHTNEOBHigh-Throughput µPAC Neo HPLC Column5.5100-250010-500 ng< 15 minOrder now ›
μPAC Neo trapping columns
Catalog numberProduct lineFor use withFlow rate range (μL/min)Sample loadingOrder
COL-TRPLOLONEOB2 (Duo-Pack)μPAC Neo low-load trapping columnμPAC Neo low-load HPLC column100-750< 10 ngOrder now ›
μPAC Neo accessories
Catalog numberProduct lineFor use withOrder
6040.2304Union for Viper and nanoViper TubingDouble nanoViper and μPAC Neo ColumnsOrder now ›
6041.5260nanoViper tubing 20 μm x 550 mmDouble nanoViper and μPAC Neo ColumnsOrder now ›
ES993Nano EASY-Spray emitter, 10 μm I.D.Double nanoViper and μPAC Neo ColumnsOrder now ›
µPAC Gen 1 HPLC columns

These HPLC columns are ideally suited for bottom-up proteomics where resolution is critical to the success of the analysis. Our µPAC HPLC columns provide excellent column-to-column reproducibility and peak capacity at a wide range of flow rates.

Catalog numberProduct linePillar diameter (µm)Interpillar distance (µm)Column length (cm)Flow rate range (µL/min)Order
COL-nano050G1BµPAC pillar array52.5500.1 – 2.0Order now ›
COL-nano200G1BµPAC pillar array52.52000.1 - 1.0Order now ›
µPAC traps
Catalog numberProduct linePillar diameter (µm)Interpillar distance (µm)Column length (cm)Order
COL-trpnano16G1B2 (Duo-Pack)µPAC pillar array52.51Order now ›
µPAC accessories
Catalog numberProduct lineFor use withOrder
EMI-flexionBμPAC Flex iON Connect ESI-MS: Interface to connect μPAC nanoLC columns to a Nanospray Flex Series ion sourceμPAC nano columnsOrder now ›
EMI-easysprayBμPAC Compatible EASY-Spray Emitter: Pre-assembled EASY-Spray emitter, grounding union, and transfer line to connect μPAC nanoLC columns to an EASY-Spray sourceμPAC nano columnsOrder now ›

Thermo Scientific Vanquish Neo UHPLC System

The all-in-one nano-, capillary-, and micro-flow LC system for high sensitivity LCMS workflows.

Learn more

Biognosys AG: MAbPac RP 4 μm stand-alone and EASY-Spray LC columns and nano- and capillary-flow emitters

 

Technical University of Denmark (DTU): MAbPac RP 4 μm stand-alone and EASY-Spray LC columns and nano- and capillary-flow emitters

 

 
 
 
 
 
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Low-flow chromatography consumables reference guide for LC-MS proteomics research

Download this guide and learn more about the portfolio of Thermo Scientific consumables for LC-MS proteomics research and the value they can bring to your lab.

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