Online SPE

Clean-up and concentrate target sample analytes online in minutes

Our online SPE columns contain popular Thermo Scientific HyperSep SPE chemistries to retain specific analytes in a sample matrix. Use these online SPE columns with an appropriate HPLC column and switching valves.

  • Effective removal of contaminants such as proteins from samples
  • Compatible with conventional HPLC systems
  • Fast and effective clean-up and concentration of target compounds

Online Solid Phase Extraction Columns

Thermo Scientific online SPE Columns are available in several convenient formats for sample preparation and provide quick, easy connection by means of a reusable drop-in guard cartridge holder.

  • HyperSep Retain PEP for retention of polar and nonpolar analytes
  • HyperSep Retain CX for retention of basic and nonpolar analytes
  • HyperSep Retain AX for retention of acidic and nonpolar analytes
  • HyperSep Hypercarb for retention of extremely polar analytes

HyperSep Javelin direct-connect online SPE columns

ID (mm)Length (mm)Retain REPRetain CXRetain AXHypercarbQuantity
2.11060310-20160310-30160310-40160310-5014 Pack
3.01060310-20260310-30260310-40260310-5024 Pack

HyperSep UniGuard direct-connect online SPE columns

ID (mm)Length (mm)Retain REPRetain CXRetain AXHypercarbQuantity
2.11060311-20160311-30160311-40160311-5014 Pack
3.01060311-20260311-30260311-40260311-5024 Pack

HyperSep HPLC columns for online SPE

ID (mm)Length (mm)Retain REPRetain CXRetain AXHypercarbQuantity
2.12060312-20160312-30160312-40160312-5011 Each
3.02060312-20260312-30260312-40260312-5021 Each

Ultimate guide to solid phase extraction

This 144-page guide includes:

What you need to know about:Methods for:
  • SPE Phase Selection
  • SPE Workflow
  • Solvent selection
  • Method development & optimization
  • Pharmaceutical/Biotech
  • Environmental
  • Forensic
  • Food Safety

Download guide to solid phase extraction

Resources