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ProQuantum High-Sensitivity Immunoassays 
A powerful innovation in protein quantitation
Invitrogen ProQuantum immunoassays are designed for easy-to-run, high-performance, target-specific protein detection, with no proprietary instrument to purchase. Utilizing proximity-based amplification technology, ProQuantum assays offer the analyte specificity of antibody-antigen binding with the signal detection and amplification capabilities of qPCR to achieve a highly sensitive assay. ProQuantum assays can also typically detect lower levels of protein with lower sample consumption than traditional methods.
Time to try ProQuantum High-Sensitivity Immunoassays
ProQuantum immunoassays benefits at-a-glance:
- High-sensitivity—detect low levels of proteins with greater sensitivity than traditional methods (Figure 1)
- Broad dynamic range—≥5 logarithmic units, minimizing sample dilutions to ensure they fall within the range
- Small sample consumption—use 2–5 μL of sample (instead of 75 μL for triplicate wells with other methods)
- Fast, easy workflow—typically 2 hours from sample to answer, no wash steps, and a single 1-hour incubation
- No proprietary instrument to purchase—runs on any real-time PCR instrument
- Includes intuitive cloud-based software—comprehensive data analysis and statistical group–wise comparison
Click image to enlarge
Figure 1. Standard curve for Invitrogen ProQuantum High-Sensitivity Human IFNγ, IL-1β, IL-8, and IL-6 immunoassay kits (red) shows larger dynamic range and improved separation at the low end of the curve for greater sensitivity than normal and ultrasensitive ELISAs (blue).
Watch the on-demand webinars to get the technical details on the ProQuantum platform and its applications.
Immunoassay Research Areas
Explore our immunoassays for different research areas, including immune-oncology, neurobiology, isotyping and immunoglobulin characterization, hallmarks of cancer, car t cells, and more.
Download the product brochure or watch the instructional video
To learn more about the technology and benefits of ProQuantum high-sensitivity immunoassays, how easy it is to run an assay and how they can help you in your research, download the ProQuantum high-sensitivity immunoassays brochure or watch the instructional video by clicking the appropriate button below:
To learn more, email us at invitrogenimmunoassays@thermofisher.com and we will connect you with a representative who can help.
ProQuantum immunoassays utilize a matched pair of target-specific antibodies each conjugated to a DNA oligonucleotide. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology. During antibody–analyte binding, the two DNA oligos are brought into close proximity, which then allows for ligation of the two strands and subsequent creation of template strand for amplification (Figure 2).
Click image to enlarge
Figure 2. ProQuantum immunoassay schematic.(A) Antibody-conjugates binding to target (1-hr incubation). (B) Ligation and amplification of signal (in qPCR instrument).
Watch the “How to run a ProQuantum assay” instructional video for simple steps to run the assay at your lab bench.
ProQuantum Software analyzes target-specific protein quantitation results for all ProQuantum high-sensitivity immunoassay kits. The software provides end-to-end support starting with step-by-step guidance to prepare an assay run including setting up the standard curve and plate layout for 96- or 384-well plates. The analysis features include standard curve qualification, with built-in outlier detection, interpolation of unknown samples for quantitation, and group-wise statistical analysis.
- Easily design your experimental 96 or 384-well plate run with automatic calculations for standard curve dilutions and suggested plate plans
- Get customized reaction volumes and lab instructions based on your plate plan (Cloud only)
- 4 or 5PL algorithm standard curve fit with built-in outlier detection
- Easily view unknown sample concentrations from standard curve graphs or in table format
- Statistical group-wise comparison
- Export data to .pdf, .xls or GraphPad Prism
A - Standard curve wizard
B - Design plate layout
C - Customized assay instructions
D - Analyzed data
E - Groupwise statistical comparison
Figure 3. Intuitive software for easy and comprehensive data analysis
ProQuantum Software v1.0
Desktop version
All the features of the Cloud-based version except kit-specific auto populated details and customized kit-protocols.
Cloud-based version
Get free access to the cloud-based version of the ProQuantum software on Thermo Fisher Cloud.
To try the software with a ProQuantum data file, use this sample .eds data file to upload. Please click on create experiment (Figure 4), Type in Cat. No. A35601 for the autopopulated default settings, select duplicate and click next. Create a new plate plan according to this image (Figure 5) by typing in the number 30 in the Samples text box. Then, import the .eds file (Figure 6) and select your plate plan to apply for analysis.
Figure 4.
Figure 5.
Figure 6.
Thermo Fisher Scientific is committed to designing our products with the environment in mind—it’s part of how we enable our customers to make the world healthier, cleaner, and safer. This fact sheet provides the rationale behind the environmental claim that Invitrogen ProQuantum Immunoassay Kits generate less waste and have more sustainable packaging than traditional ELISA kits. To learn more download the green features fact sheet
Need a custom ProQuantum assay? Lot reservation? Special bulk packaging?
Let us fulfill your special request
We provide custom target development services of your chosen protein targets and immunoassay platform, as well as, lot reservations and custom packaging for bulk orders.
Performance guarantee
If an Invitrogen immunoassay does not perform as described on our data sheet, we will replace the product at no cost to you, or if you prefer, we will provide you with a credit for future purchase.*
Learn more about performance guarantee
We're here to help
Our team of field application specialists are trained in immunoassay workflows. We can help you choose an assay or configure a panel, optimize an experiment, troubleshoot and more – face to face or on the phone at no cost.
Contact a field application specialistFor Research Use Only. Not for use in diagnostic procedures.