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Zinc concentrations in the 1–100 nM range are routinely measured using fluorescent indicators nominally designed for Ca2+ detection, but higher Zn2+ concentrations are present in synaptic vesicles and released in response to electrical stimulation or excitotoxic agonists.
We’ve developed a number of Molecular Probes ion indicators to track zinc with intense fluorescent signals and a range of wavelength options.
Indicators for Zinc
- Optimized for higher Zn2+ concentrations
- Compatible with multiple analysis platforms
FluoZin dyes are unique indicators designed for detection of Zn2+ in the 0.1–100 µM range with minimal interfering Ca2+ sensitivity. They provide bright signals for assaying exocytotic release of Zn2+ from pancreatic β-cells after stimulation with glucose or potassium. Zn2+ concentrations can be measured using imaging platforms, cell-based microplate assays, or flow cytometry.
Human vascular smooth muscle cell loaded with FluoZin indicator and stained with Hoechst 3342.
Used for spatial and temporal resolution of Zn2+ concentrations.
| FluoZin-1, Tripotassium Salt | FluoZin-1, AM | FluoZin-3, Tetrapotassium Salt | FluoZin-3, AM | |
|---|---|---|---|---|
| Readout | Responds to Zn2+ concentrations in synaptic vesicles with increasing fluorescence | Responds to saturating levels of Zn2+ with 50-fold increase in fluorescence emission | ||
| Range | 0–100 µM range with minimal interfering Ca2+ sensitivity | Low nM to µM range with minimal interfering Ca2+ sensitivity | ||
| Fluorophore | FluoZin-1 | FluoZin-3 | ||
| Standard filter set | FITC | |||
| Ex/Em (nm) | 495/515 | 494/516 | ||
| Bibliography | Citations | Citations | ||
| Cell permeant or impermeant? | Impermeant | Permeant | Impermeant | Permeant |
| Platforms | Imaging | Imaging | Imaging | Imaging |
| Usage notes | Load by microinjection, patch pipette, or pinocytic loading agent | Load in cell media | Load by microinjection, patch pipette, or pinocytic loading agent | Load in cell media |
| Format | 500 µg | 50 µg | 500 µg | 100 µg |
| Cat. No. | F24180 | F24181 | F24194 | F24195 |
Resources
Molecular Probes Handbook
More Information
For Research Use Only. Not for use in diagnostic procedures.