96-Well Cell Culture Plates | Thermo Fisher Scientific

19 96 well plates—some in white and some in black

Select 96-well cell culture plates by surface

Whether culturing individual cell lines or scaling up for high-throughput screening, there is a Thermo Scientific Nunc 96-well plate for your needs. Advances in surface modifications, well geometry and optical clarity mean a variety of 96-well cell culture plate tailored for your specific application, including optical bottom plates for cellular imaging.

To help ensure reliability, flexibility, and reproducibility across every stage of cell culture, plates are available with a variety of surfaces optimized for different needs in cell attachment and cell growth.

Nunc Edge 2.0 96-well plate 96-well optical bottom plates

Non-treated

Non-treated surface for suspension culture and general biochemical assays

Made from high-quality USP Class VI virgin polystyrene, solid Nunc 96-well plates (in white and black) are ideal for colorimetric, fluorescence-, and luminescence-based cell analysis as well as protein binding assays

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Clear, flat bottom, 96-well non-treated plates
Black, flat bottom, polystyrene 96-well non-treated plates
White, flat bottom, polystyrene 96-well non-treated plates
Clear, round (U) bottom, polystyrene 96-well non-treated plates
Clear, conical (V) bottom, polystyrene 96-well non-treated plates

Clear, flat bottom 96-well non-treated plates

Cat. No.	Description	Surface	Color	Lid	Sterile	Pack size	Case size
269620	Nunc 96-Well Flat Bottom	Non-treated	Clear	No	No	Sleeve of 5	60	Order
260860	Nunc 96-Well Flat Bottom	Non-treated	Clear	Yes	Yes	Sleeve of 10	160	Order
266120	Nunc 96-Well Flat Bottom	Non-treated	Clear	Yes	Yes	Sleeve of 25	100	Order
243656	Nunc 96-Well Flat Bottom	Non-treated	Clear	Yes	Yes	Sleeve of 6	108	Order
269787	Nunc 96-Well Flat Bottom	Non-treated	Clear	No	Yes	Individually packed	50	Order
260895	Nunc 96-Well Flat Bottom	Non-treated	Clear	Yes	No	Sleeve of 10	160	Order
260844	Nunc 96-Well Flat Bottom, Barcoded	Non-treated	Clear	No	No	Sleeve of 10	180	Order
260887	Nunc 96-Well Flat Bottom, Barcoded	Non-treated	Clear	Yes	Yes	Sleeve of 10	160	Order
260836	Nunc 96-Well Flat Bottom	Non-treated	Clear	No	No	Sleeve of 10	180	Order

Sleeve of 10

Sleeve of 10

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Black, flat bottom, polystyrene 96-well non-treated plates

Cat. No.	Description	Surface	Color	Lid	Sterile	Pack Size	Case Size
237108	Nunc 96-Well Flat Bottom	Non-treated	Black	No	No	Sleeve of 10	180	Order
237105	Nunc 96-Well Flat Bottom	Non-treated	Black	Yes	Yes	Individually packed	50	Order
237107	Nunc 96-Well Flat Bottom	Non-treated	Black	Yes	Yes	Sleeve of 10	160	Order

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White, flat bottom, polystyrene 96-well non-treated plates

Cat. No.	Description	Surface	Color	Lid	Sterile	Pack Size	Case Size
236108	Nunc 96-Well Flat Bottom	Non-treated	White	No	No	Sleeve of 10	180	Order
236105	Nunc 96-Well Flat Bottom	Non-treated	White	Yes	Yes	Individually packed	50	Order
236107	Nunc 96-Well Flat Bottom	Non-treated	White	Yes	Yes	Sleeve of 10	160	Order

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Clear, round (U) bottom, polystyrene 96-well non-treated plates

Cat. No.	Description	Surface	Color	Lid	Sterile	Pack Size	Case Size
268152	Nunc 96-Well U Bottom	Non-treated	Clear	No	No	Sleeve of 10	180	Order
268200	Nunc 96-Well U Bottom	Non-treated	Clear	Yes	Yes	Sleeve of 10	160	Order
262162	Nunc 96-Well U Bottom	Non-treated	Clear	No	Yes	Individually packed	50	Order
278606	Nunc 96 Deep Well U Bottom	Non-treated	Clear	No	Yes	Sleeve of 4	32	Order
278605	Nunc 96 Deep Well U Bottom	Non-treated	Clear	No	No	Sleeve of 4	32	Order
262146	Nunc 96-Well U-Bottom	Non-treated	Clear	No	No	Bulk pack of 135	135	Order

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Clear, conical (V) bottom, polystyrene 96-well non-treated plates

Cat. No.	Description	Surface	Color	Lid	Sterile	Pack Size	Case Size
249570	Nunc 96-Well V Bottom	Non-treated	Clear	No	No	Sleeve of 10	180	Order
249662	Nunc 96-Well V Bottom	Non-treated	Clear	No	Yes	Individually packed	50	Order
249952	Nunc 96-Well V Bottom	Non-treated	Clear	Yes	Yes	Sleeve of 10	160	Order
249935	Nunc 96-Well V Bottom	Non-treated	Clear	Yes	Yes	Sleeve of 10	80	Order
249940	Nunc 96-Well V Bottom	Non-treated	Clear	No	Yes	Sleeve of 10	80	Order

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Nunclon Delta surface

Nunc 96-well cell culture treated plates for adherent cells

A macro of a Nunclon Delta cultureware surface

The Nunclon Delta surface is a fully synthetic energy-treated surface that makes the otherwise very hydrophobic polystyrene surface more hydrophilic, thus facilitating cell attachment and growth. Our Nunclon Delta products are subjected to rigorous quality control procedures to meet the standards of Thermo Fisher Scientific and to ensure lot-to-lot consistency.

Explore cell performance with Nunclon Delta surface

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Flat bottom 96-well cell-culture-treated plates
Round (U) bottom 96-well cell-culture-treated plates

Flat bottom, polystyrene 96-well cell-culture-treated plates

Cat. No.	Description	Surface	Color	Lid	Sterile	Pack size	Case size
167008	Nunc 96-Well Flat Bottom	Nunclon Delta	Clear	Yes	Yes	Individually packed	50	Order
161093	Nunc 96-Well Flat Bottom	Nunclon Delta	Clear	Yes	Yes	Sleeve of 10	160	Order
136101	Nunc 96-Well Flat Bottom	Nunclon Delta	White	Yes	Yes	Individually packed	50	Order
136102	Nunc 96-Well Flat Bottom	Nunclon Delta	White	Yes	Yes	Sleeve of 10	160	Order
137101	Nunc 96-Well Flat Bottom	Nunclon Delta	Black	Yes	Yes	Individually packed	50	Order
137103	Nunc 96-Well Flat Bottom	Nunclon Delta	Black	Yes	Yes	Sleeve of 10	160	Order
168055	Nunc 96-Well Flat Bottom	Nunclon Delta	Clear	No	Yes	Individually packed	50	Order

Round (U) bottom, polystyrene 96-well cell-culture-treated plates

Cat. No.	Description	Surface	Color	Lid	Sterile	Pack size	Case size
163320	Nunc 96-Well U Bottom	Nunclon Delta	Clear	Yes	Yes	Individually packed	50	Order
168136	Nunc 96-Well U Bottom	Nunclon Delta	Clear	Yes	Yes	Sleeve of 10	160	Order
143761	Nunc 96-Well U Bottom	Nunclon Delta	Clear	No	Yes	Individually packed	50	Order

Nunclon Sphera surface

Nunclon Sphera surface-treated plates for ultra-low binding surface

A macro of a Nunclon Sphera cultureware surface

The Nunclon Sphera surface-treated plates offer a physiologically relevant 3D cell culture format for disease modeling and drug screening applications in cancer and stem cell research, designed to enable the production of reproducible spheroids that can be further differentiated into specific organoids.

Explore the Nunclon Sphera surface and request samples

Cat. No.	Description	Surface	Color	Lid	Sterile	Pack size	Case size
174925	Nunclon Sphera 96-Well U Bottom	Nunclon Sphera	Clear	Yes	Yes	Individually packed	8	Order
174929	Nunclon Sphera 96-Well U Bottom	Nunclon Sphera	Clear	Yes	Yes	Sleeve of 5	50	Order
174927	Nunclon Sphera 96-Well Flat Bottom	Nunclon Sphera	Clear	Yes	Yes	Individually packed	8	Order

Nunclon Supra surface

Nunc 96-well Supra treated plates for adherent cells

Thermo Scientific Nunc 96-well plates with Supra surface treatment are designed for culturing finicky adherent cells (primary and MSCs). When results matter most, Supra surface is designed to deliver higher cell yield, reduced time-to-adherence, and better morphology.

Nunclon Supra surface is more hydrophilic than the Nunclon Delta surface, enhancing the growth of finicky cell types under no-serum (xeno-free culture) or low-serum conditions.

See how Supra performs on 39 cells/cell lines

Nunc UpCell surface

Nunclon UpCell surface-treated plates for trypsin free dissociation

A macro of a Nunc UpCell cultureware surface

The Nunc UpCell surface-treated plates are grafted with PIPAAm polymers that change conformation and hydrophilicity according to external temperature, dislodging cells from the growth surface in this process while keeping cellular membrane and surface receptors intact.

Explore the Nunclon UpCell surface

Nunc UpCell 96-well plate for culturing and harvesting adherent cells without trypsin

Cat. No.	Description	Surface	Color	Lid	Sterile	Pack size	Case size
174897	Nunc UpCell 96 Flat Bottom	UpCell	Clear	Yes	Yes	Individually packed	8	Order

ECM surface

Nunc ECM treated plates for finicky cells

A macro of a Nunc ECM cultureware surface

Nunc poly-D-lysine (PDL)- and collagen I-coated plates are designed to enhance the adhesion and growth of primary cells and finicky cell lines in vitro. The PDL synthetic material and the collagen I protein of animal origin are aseptically coated in either the Nunc regular plates or the Nunc Optical Bottom plates with 10-3 sterility assurance level (SAL).

Explore PDL- and collagen I-coated surfaces

Cat. No.	Description	Surface	Color	Lid	Sterile	Pack size	Case size
152038	Nunc 96-Well Flat Bottom	Collagen I	Clear	Yes	Yes	Sleeve of 5	20	Order
152039	Nunc 96-Well Flat Bottom	Poly-D-Lysine	Clear	Yes	Yes	Sleeve of 5	20	Order
152036	Nunc 96-Well Optical Polystyrene Polymer Bottom	Collagen I	Black	Yes	Yes	Sleeve of 5	20	Order
152040	Nunc 96-Well Optical Polystyrene Polymer Bottom	Collagen I	White	Yes	Yes	Sleeve of 5	20	Order
152037	Nunc 96-Well Optical Polystyrene Polymer Bottom	Poly-D-Lysine	Black	Yes	Yes	Sleeve of 5	20	Order
152028	Nunc 96-Well Optical Polystyrene Polymer Bottom	Poly-D-Lysine	White	Yes	Yes	Sleeve of 5	20	Order

Nunc Edge 2.0 96-well plate with moat

Thermo Scientific Nunc Edge 2.0 96-well cell culture plates contain a large evaporation moat that can be filled with sterile water or media to help eliminate edge effect.

This edge effect is caused when evaporation occurs in the perimeter wells of a microplate during prolonged incubation. It changes media pH, osmolality, and the concentration of the media constituents, affecting cell viability and function.

However, the built-in moat in the Nunc Edge 2.0 plate significantly reduces evaporation compared to the conventional 96-well plates after four-day incubation, improving well-to-well consistency.

A chart showing superior cell viability when using Edge 2.0 plates

To prevent evaporation, simply:

A 96-well plate being filled with desired cell culture

Utilize all 96 wells

A Nunc Edge 2.0 plate exterior moat in the process of being filled

Fill surrounding moat with 2 mL
sterile water or media in each section

A close-up image of water evaporating from the 2.0 moat but not from the wells themselves.

Watch as evaporation happens in the moat and not the outer wells

Order Nunc Edge 2.0 96-well plates

Cat. No.	Description	Surface	Color	Lid	Sterile	Pack size	Case size
167425	Nunc Edge 2.0 96-Well Plate Treated	Nunclon Delta	Clear	Yes	Yes	Individually packed	50	Order
167542	Nunc Edge 2.0 96-Well Plate Treated	Nunclon Delta	Clear	Yes	Yes	Sleeve of 10	160	Order
167574	Nunc Edge 2.0 96-Well Plate Treated	Nunclon Delta	Clear	No	Yes	Individually packed	50	Order
167554	Nunc Edge 2.0 96-Well Plate Treated	Nunclon Delta	Clear	No	Yes	Sleeve of 10	160	Order
267427	Nunc Edge 2.0 96-Well Plate Non-treated	Non-treated	Clear	Yes	Yes	Individually packed	50	Order
267544	Nunc Edge 2.0 96-Well Plate Non-treated	Non-treated	Clear	Yes	Yes	Sleeve of 10	160	Order
267576	Nunc Edge 2.0 96-Well Plate Non-treated	Non-treated	Clear	No	Yes	Individually packed	50	Order
267556	Nunc Edge 2.0 96-Well Plate Non-treated	Non-treated	Clear	No	Yes	Sleeve of 10	160	Order
267566	Nunc Edge 2.0 96-Well Plate Non-treated	Non-treated	Clear	No	No	Sleeve of 10	160	Order
267578	Nunc Edge 2.0 96-Well Plate Non-treated	Non-treated	Clear	Yes	No	Sleeve of 10	160	Order

Sleeve of 10

Sleeve of 10

96-well plate with optical bottom

Optical bottom plates for cellular imaging

With superior imaging quality and minimal background noise and crosstalk between wells, Nunc optical bottom plates are optimized for fluorescence and luminescence imaging applications.

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Optical polymer film bottom 96-well plates
Optical coverglass bottom 96-well plates

Optical polystyrene polymer bottom, 96-well plates

Cat. No.	Description	Surface	Color	Lid	Sterile	Pack size	Case size
165305	Nunc 96-Well Optical Polystyrene Polymer Bottom	Nunclon Delta	Black	Yes	Yes	Sleeve of 10	30	Order
165306	Nunc 96-Well Optical Polystyrene Polymer Bottom	Nunclon Delta	White	Yes	Yes	Sleeve of 10	30	Order
152036	Nunc 96-Well Optical Polystyrene Polymer Bottom	Collagen I	Black	Yes	Yes	Sleeve of 5	20	Order
152040	Nunc 96-Well Optical Polystyrene Polymer Bottom	Collagen I	White	Yes	Yes	Sleeve of 5	20	Order
152037	Nunc 96-Well Optical Polystyrene Polymer Bottom	Poly-D-lysine	Black	Yes	Yes	Sleeve of 5	20	Order
152028	Nunc 96-Well Optical Polystyrene Polymer Bottom	Poly-D-lysine	White	Yes	Yes	Sleeve of 5	20	Order
265301	Nunc 96-Well Optical Polystyrene Polymer Bottom	Non-treated	Black	No	No	Sleeve of 10	30	Order
265302	Nunc 96-Well Optical Polystyrene Polymer Bottom	Non-treated	White	No	No	Sleeve of 10	30	Order

Optical coverglass bottom, 96-well plates

Cat. No.	Description	Surface	Color	Lid	Sterile	Pack size	Case size
164588	Nunc 96-Well Optical 1.5 Coverglass Bottom	Cell culture-treated	Black	Yes	Yes	Sleeve of 6	30	Order
164590	Nunc 96-Well Optical 1.5 Coverglass Bottom	Cell culture-treated	White	Yes	Yes	Sleeve of 6	30	Order
160376	Nunc 96-Well Optical 1.5 CC² Coverglass Bottom	CC² enhanced cell culture	Black	Yes	Yes	Sleeve of 6	30	Order
265300	Nunc 96-Well Optical 1.5 Coverglass Bottom	Non-treated	Black	No	No	Sleeve of 5	30	Order

Resources

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Surface selection: Achieving optimal cell growth and harvesting—Get an overview of technologies used to modify cultureware surfaces to help you select the right surface for your application.
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