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Gibco Tet-System Approved FBS has been functionally tested to provide researchers with optimal control over their gene expression systems, thus minimizing challenges that can be posed by this type of reagent.
Functional, cell-based assays that detect the presence of tetracycline and its derivatives are used to select FBS that delivers the highest range of induction.
Tet-approved FBS can be used in many applications.
Research area | Example |
---|---|
Neuroscience | Produce GABAA receptors for electrophysiological study [1] |
Cancer research | Quantitatively control PVT1 expression by tet-on in bladder cancer [2] Tetracycline-regulated shRNA expression to apply to monocyte interaction [3] |
Drug screening | Establish cell line with inducible Glutamate receptor for drug screening [4] |
Vaccine development | Research for the safer vaccinia virus vaccines with tet-inducible system [5] |
Gene editing | CRISPR/Cas9-mediated control of NFAT5 [6] and PVT1 and ANRIL [7] |
A biomanufacturing organization looking to improve the safety of cell culture virus production has engineered a cell line that will only permit virus expression in the presence of tetracycline. Replacing traditional FBS with tet-approved FBS (tetracycline-free FBS) improves control over when the virus is produced.
FBS used | Condition | Result |
---|---|---|
Tet-System Approved FBS | -Tet | |
Tet-System Approved FBS | +Tet | |
Standard FBS with residual tetracycline | -Tet | |
Standard FBS with residual tetracycline | +Tet |
Use of tet-approved FBS helps provide optimal control of the virus production.
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For Research Use or Further Manufacturing Use only. Serum and blood proteins are not for direct administration into humans or animals.