Choosing a vector for the delivery of your gene

• Clone into a basic shuttle vector, pMX
• Clone into a protein expression vector
• Clone into any one of a broad range of vectors, including Invitrogen vectors
• Send us your personal vector

If you’d prefer to start with synthetic linear DNA, we offer affordable and high-quality Strings DNA fragments delivered fast and ready to clone yourself.

Our standard shuttle vector, pMX, is a pUC19 derivative with a choice of antibiotic resistance markers. These vectors are intended for delivery of your clonal synthetic DNA. You simply excise your gene via restriction enzymes or PCR amplification and clone as you wish. Any specific flanking restriction enzymes you desire should be included in the gene sequence design provided for de novo synthesis.  

Get your de novo gene cloned into our pMX vector using GeneArt Instant Designer

To qualify for direct cloning, genes must be <5 kb and must not contain complex sequences. To optimize your gene sequence, reduce complexity, and maximize protein expression levels, use the Invitrogen GeneArt GeneOptimizer.

Express delivery and Super Speed service saves additional production time if you need your clone fast. We offer many options that make it possible to obtain your cloned, expression-ready genes fast with the same high quality you rely on.

We can clone your de novo gene into one of the many commercially available Invitrogen vectors that cover a multitude of downstream applications. If you don’t see a vector for selection from the drop-down in the classic ordering portal, you can purchase a commercially available Invitrogen vector and send it to us. 

We can also subclone your gene into virtually any vector you send us. Simply download the sequence submission form and send to geneartsupport@thermofisher.com and we will respond with a quote for the de novo gene synthesis and cloning into your desired vector.