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BigDye Terminator v3.1 Cycle Sequencing Kit
Precise, flexible, and reliable cycle sequencing. It’s in our DNA.
With high-quality chemistry and enhanced productivity, the BigDye Terminator v3.1 Cycle Sequencing Kit delivers improved agility and accuracy. Achieve greater uniform peak heights, longer and higher-quality reads, and more rigorous base assignments for heterozygote and mutation detection, all safe in the knowledge that you’re fully supported. As your partner, we’re with you, every step of the way.
BigDye Terminator v3.1 Cycle Sequencing Kit—robust, flexible chemistry made simple
Created by experts and refined by global teams, the BigDye Terminator v3.1 cycle sequencing kit offers exceptional versatility and high performance. This is a comprehensive, exhaustive solution, built for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
Improved quality of results and success rates, particularly with challenging templates
- Suitable for a wide range of sequencing applications
- Optimized for long read lengths
- Sophisticated dye mobility characteristics
- Refined performance reading through GT-rich regions
- Optimal signal balance
- Rapid completion of sequencing projects
The BigDye Terminator v3.1 Cycle Sequencing Kit is expertly designed to meet the demands of the vast majority of contemporary applications, including de novo sequencing and resequencing. Consistently generating data with reliably uniform peak heights and optimized signal balance, the BigDye Terminator kit produces long, high-quality reads, and more accurate base assignments for heterozygote and mutation detection.
The chemistry’s powerful formulation is compatible with a variety of templates, including PCR products, plasmids, and large insert clones, such as fosmids and bacterial artificial chromosomes (BACs). However, its dyes are the same as those found in the v3.0 and v1.0/v2.0 kits respectively, meaning no new software or instrument recalibration is required for data analysis. All that’s left to do is integrate v3.1 into your workflow and rest assured that you’ll reap the rewards of its advanced chemistry profile and ease of use.
Chemistry that performs and adapts to your needs
The challenges of cycle sequencing call for consumables you can trust to deliver. All BigDye sequencing reagents are rigorously tested twice for quality: first for correct formulation and, finally, for consistent performance on our sequencing systems. And if you should need any assistance? Our expert Applied Biosystems field and telephone support teams are always on hand to advise and answer your questions.
With BigDye Terminator, you’re in control
BigDye Terminator v3.1 and v1.1 cycle sequencing kits are completely comprehensive and include all required reagents for sequencing 24, 100, 1,000, 5,000, or 25,000 single stranded (ss) or double-stranded (ds) DNA templates. The reagents in each kit are optimized for use with the 3730xl, 3500, SeqStudio, and Seqstudio Flex genetic analyzers.
| BigDye Terminator v3.1 | BigDye Terminator v1.1 |
Format | 96- or 384-well plate, 8-well strips, 12-well strips | 96- or 384-well plate, 8-well strips, 12-well strips |
For use with | 3730xl DNA Analyzer, GeneAmp 9700, Veriti Thermal Cycler, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer. SeqStudio Genetic Analyzer, SeqStudio Flex Genetic Analyzer | 3730xl DNA Analyzer, GeneAmp 9700, Veriti Thermal Cycler, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer, SeqStudio Genetic Analyzer, SeqStudio Flex Genetic Analyzer |
Includes | 10 x 20 mL tubes of BigDye Terminator v3.1 Ready Reaction Mix, 4 tubes M13 (-21) Primer, 4 tubes pGEM Control DNA, 1 x 233 mL tube of 5X Sequencing Buffer (may be stored at 4°C) | 2 × 20 mL tubes of BigDye Terminator v1.1 Ready Reaction Mix, 2 tubes of M13 (-21) Primer, 2 tubes of pGEM Control DNA, one 28-mL tube of 5X Sequencing Buffer (may be stored at 4°C) |
No. of reactions | 25,000 | 5,000 |
Stability/storage | Store at -15 to -25°C | Store at -15 to -25°C |
Purchase | Purchase |
Comparison of BigDye Terminator v3.1 Cycle Sequencing Kit to Alternative Product for Challenging Templates
Explore a comparative study evaluating the performance of the BigDye Terminator v3.1 Kit against an alternative product for sequencing challenging templates.
Application Note: A Workflow for Obtaining High Quality Sequencing Data from Bacterial Artificial Chromosome (BAC) DNA
Bacterial artificial chromosome (BAC) DNA is difficult to use as a sequencing template, because of its large size (usually >100,000 bp). A modified sequencing protocol has been demonstrated for BAC DNA using the BigDye Terminator Cycle Sequencing Kit. Read how the implementation of the BigDye XTerminator Purification Kit for reaction cleanup resulted in increased signal intensity and overall reading quality of the sequence data, comparable to that from plasmid templates.
The BigDye Terminator v1.1 and v3.1 chemistries can be diluted, and a 5X Sequencing Buffer is provided with the kit to enable you to do so.
The BigDye Direct Cycle Sequencing Kit and the dGTP BigDye Terminator Ready Reaction Kits v1.0 and v3.0 cannot be diluted.
Brochure: Sanger sequencing—Generate high-quality data with our proven workflow
Possible causes could be oxidation or a change in pH when injecting out of water or old Hi-Di formamide that has broken down. Some cleanup methods can also oxidize the dyes. Alternatively, an old or contaminated array can cause dye breakdown, while an arcing event on the system can destabilize the blue baseline and, in severe cases, mask it completely.
It’s all about choosing the right chemistry for your application. If your research requires the most robust, flexible chemistry that will generate the longest reads, then the v3.1 kit is most suitable. If your laboratory is primarily sequencing short PCR fragments using rapid electrophoresis-run modules, then the v1.1 kit would serve you better. Many variables contribute to DNA sequencing data quality, including template type, instrument module, total signal, peak-height uniformity, and mobility shift. All should be taken into consideration when selecting the most appropriate chemistry for your work.
Learn about the Sanger sequencing workflow ›
Instruments for Sanger sequencing and fragment analysis ›
Sanger Analysis Modules Sanger sequencing and fragment analysis software ›
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