Antibodies for Pluripotent Stem Cell Detection

Validating cell line quality is critical to stem cell research. Therefore, we offer you maximum flexibility in antibody and dye choices to enable you to specifically detect your pluripotent stem cells (PSCs) and their derivatives.

Superior imaging for PSCs in one box

Everything you need for superior image-based analysis of hPSCs and a variety of lineages is now available in one box. Introducing Molecular Probes Immunocytochemistry and live imaging kits for stem cells. Kits offer a combination of antibodies, stains, buffers, and/or media to create beautiful, high-quality images of stem cells.

Pluripotent state

Differentiated state

Four panel stem cell imaging results showing SSEA4 expression, OCT4 expression, DAPI staining, and overlay of all images.
Figure 1. Analysis of OCT4 and SSEA4 expression in iPSCs using the PSC 4-Marker ICC Kit. Induced pluripotent stem cells (iPSCs) derived from CD34+ cord blood (Gibco Human Episomal iPSC Line) were grown under feeder-free conditions using Essential 8 Medium in wells coated with vitronectin. The cells were stained for pluripotency markers using the PSC 4-Marker ICC Kit.

 
Image shows a merge of the red and green channels. A PSC colony stained red using the TRA-1-60 antibody is surround by feeder cells, stained green with the CD44 antibody.
Figure 2. Surface marker analysis of feeder-dependent PSC cultures using dye-conjugated antibodies for TRA-1-60  (red) as a positive marker and CD44 (green) as a negative marker for pluripotency. 
Stained PSC-derived cardiomyocytes showing NKX2.5 marker (red), TNNT2/c TNT marker (green), and DAPI nuclear staining (blue).
Figure 3. iPSCs were differentiated for 14 days using the PSC Cardiomyocyte Differentiation Kit. The cells were stained using the Human Cardiomyocyte Immunocytochemistry Kit for the following markers: NKX2.5 (red) for early cardiac mesoderm and TNNT2/cTNT (green) for cardiomyocytes along with DAPI nuclear DNA counterstaining (blue).

Stand-alone Pluripotent Stem Cell Antibodies

Target AntigenReactivityHostClonalityValidationCat. No.
E-CadherinHumanMouseMonoclonalWB, IHC, IP, IF, FC, ELISA 13-1700
TRA 1-60HumanMouseMonoclonalWB, IHC, IF, FC, ELISA41-1000
TRA 1-81HumanMouseMonoclonalWB, IHC, IF, FC, ELISA41-1100
SSEA-3/4Human, MouseMouseMonoclonalIHC, IF, FC41-4000

WB = Western Blot; IHC = Immunohistochemistry; IP = Immunoprecipitation; IF = Immunofluorescence; FC = Flow Cytometry

These primary antibodies can be conjugated directly with Alexa Fluor dyes or combined with our vast suite of secondary conjugated antibodies. Stained cells can be visualized by the FLoid Cell Imaging Station (Figures 4-6).

Large colony of H9 human ESCs stained green with SSEA4 primary antibody and an AlexaFluor 488-conjugated anti-mouse lgG secondary antibody.

Figure 4. H9 hESCs were grown on feeders and stained (without fixing) with SSEA-4 primary antibody and an Alexa Fluor 488–conjugated anti–mouse IgG secondary antibody. The cells were visualized at 100x magnification using a FITC filter.

Colony of H9 human ESCs stained green with TRA-1-81 primary antibody surrounded by non-stained black MEFs.

Figure 5. H9 hESCs were grown on feeders and stained (without fixing) with TRA-1-81 primary antibody and an Alexa Fluor 488–conjugated anti–mouse IgG secondary antibody. The cells were visualized at 50x magnification. Shown is a merged image using phase contrast and a FITC filter.

Large colony of H9 human ESCs stained green with TRA-1-60 and counterstained blue with DAPI nuclear stain. Surrounded by non-stained black MEFs.

Figure 6. H9 hESCs were grown on feeders, fixed with PFA, stained with TRA-1-60 primary antibody and an Alexa Fluor 488–conjugated anti–mouse IgG secondary antibody, and counterstained with DAPI nuclear stain. The cells were visualized at 50x magnification. Shown is a merged image using phase contrast, and FITC and Hoescht filters.

For Research Use Only. Not for use in diagnostic procedures.