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Microscopy Protocols
Find step-by-step instructions for your imaging experiments. If you are just starting, we recommend the Molecular Probes School of Fluorescence as a starting point. Protocols linked below are to be used as a guide only, and may require optimization for your experiment.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Cell proliferation protocols for imaging
- BrdU Labeling and Detection Protocol
- Click-iT EdU HCS Assays Protocol
- Click-iT EdU Imaging Kits Protocol
- Click-iT Plus EdU Imaging Kits Protocol
- Click-iT EdU and Click-iT Plus EdU for In Vivo Protocol
Cell viability protocols for imaging
- Image-iT DEAD Green Kit Protocol
- LIVE BacLight Bacterial Gram Stain Kit Protocol
- LIVE/DEAD BacLight Bacterial Viability Kit Protocol
- LIVE/DEAD Cell Imaging Kit (488/570) Protocol
- LIVE/DEAD Sperm Viability Kit Imaging Protocol
- LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells Protocol
- LIVE/DEAD Yeast Viability Kit Protocol
- NucGreen Dead 488 ReadyProbes Reagent Protocol for Viability
- NucRed Dead 647 ReadyProbes Reagent Protocol for Viability
- ReadyProbes Cell Viability Imaging Kit, Blue/Green Protocol
- ReadyProbes Cell Viability Imaging Kit, Blue/Red Protocol
Cell structure protocols for imaging
- ActinGreen 488 ReadyProbes Reagent Protocol
- ActinRed 555 ReadyProbes Reagent Protocol
- DAPI Protocol for Fluorescence Imaging
- F-Actin Labeling with Fluorescent Phalloidin Protocol
- Hoechst 33342 Protocol for Imaging
- Functional Mitochondrial Staining with TMRM Protocol
- NucBlue Fixed Cell ReadyProbes Reagent Protocol
- NucBlue Live ReadyProbes Reagent Protocol
- NucGreen Dead 488 ReadyProbes Reagent Protocol for Fixed Cells
- NucRed Dead 647 ReadyProbes Reagent Protocol for Fixed Cells
- NucRed Live 647 ReadyProbes Reagent Protocol
- SYTO 59 Nuclear Staining Protocol
- SYTO 82 Nuclear Staining Protocol
- SYTOX Green Nucleic Acid Stain Protocol
- TO-PRO-3 Stain Protocol
General antibody staining protocols
- Automated Multiplex IHC Staining with Aluora Spatial Amplification Kits and Leica BOND
- Immunofluorescent Staining of Intracellular Antigens on Cultured Cells (Invitrogen eBioscience reagents)
- IHC Frozen Tissue—Direct Method (Invitrogen eBioscience reagents)
- IHC Frozen Tissue—Indirect Method (purified) (Invitrogen eBioscience reagents)
- IHC Frozen Tissue—Indirect Method (biotin) (Invitrogen eBioscience reagents)
- IHC FFPE Tissue Trypsin Digestion—Direct Method (Invitrogen eBioscience reagents)
- IHC FFPE Tissue Trypsin Digestion Antigen Retrieval—Indirect Method (Invitrogen eBioscience reagents)
- IHC FFPE Tissue Low pH Antigen Retrieval—Direct Method (Invitrogen eBioscience reagents)
- IHC FFPE Tissue Low pH Antigen Retrieval—Indirect Method (Invitrogen eBioscience reagents)
- IHC FFPE Tissue High pH Antigen Retrieval—Direct Method (Invitrogen eBioscience reagents)
- IHC FFPE Tissue High pH Antigen Retrieval—Indirect Method (Invitrogen eBioscience reagents)
- IHC Staining of Formalin-Fixed Paraffin-Embedded Tissues (Invitrogen eBioscience reagents)
- Colorimetric FFPE—High pH Antigen Retrieval (Invitrogen eBioscience reagents)
- Colorimetric FFPE—Low pH Antigen Retrieval (Invitrogen eBioscience reagents)
- Colorimetric FFPE—Trypsin Digestion (Invitrogen eBioscience reagents)
- ICC Formaldehyde Fixed Cells—Direct Method (Invitrogen eBioscience reagents)
- ICC Formaldehyde Fixed Cells—Indirect Method (Invitrogen eBioscience reagents)
- ICC Formaldehyde Fixed, Permeabilized Cells—Direct Method (Invitrogen eBioscience reagents)
- ICC Formaldehyde Fixed, Permeabilized Cells—Indirect Method (Invitrogen eBioscience reagents)
- ICC Methanol Fixed Cells—Direct Method (Invitrogen eBioscience reagents)
- ICC Methanol Fixed Cells—Indirect Method (Invitrogen eBioscience reagents)
- ICC Unfixed Cells—Direct Method (Invitrogen eBioscience reagents)
- Mounting Coverslips Protocol
- Simple Immunolabeling with Primary and Secondary Antibodies Protocol
- Tyramide staining for tissue samples
- 3D Organoid Staining
- ViewRNA tissue fluorescence assay protocol
- ViewRNA tissue colorimetric assay protocol
For Research Use Only. Not for use in diagnostic procedures.