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Pluripotent Stem Cell Protocols
At the onset of your stem cell research, we understand it can be daunting to know where to begin and what products are needed. The Pluripotent Stem Cell Protocol Handbook will provide you with the fundamental protocols, solutions, and resources needed to get you started in any workflow.
Cell reprogramming protocols
- CytoTune EmGFP Sendai Fluorescence Reporter
- CytoTune-iPS 2.0 Sendai Reprogramming Kit
- Epi5 Episomal iPSC Reprogramming Kit
- Episomal iPSC Reprogramming Vectors
- Generation of Human Induced Pluripotent Stem Cells (hiPSC) from Fibroblasts using Episomal Vectors
- Reprogramming peripheral blood mononuclear cells (PBMCs) with the CytoTune-iPS 2.0 Reprogramming Kit under feeder-free conditions
- GeneArt CRISPR Nuclease Vector Kit
Cell culture protocols
- Adaptation of hPSCs from different culture systems to Essential 8 Medium or Essential 8 Flex Medium
- Creation of embryoid bodies from iPSCs using Complete KnockOut Serum Replacement Feeder-Free Medium
- Culturing induced pluripotent stem cells (iPSCs) on MEF-conditioned medium
- Culturing PSCs in Essential 8 Medium
- Essential 6 Medium
- Essential 8 Adaptation Kit
- Essential 8 Flex Medium
- Essential 8 Medium
- Feeder-dependent culture of human embryonic stem cells (hESCs)
- Feeder-dependent culture of human induced pluripotent stem cells (hiPSCs)
- Geltrex LDEV-Free, hESC-qualified, Reduced Growth Factor Basement Membrane Matrix
- Gibco Episomal hiPSC Line
- RevitaCell Supplement
- StemScale PSC Suspension Medium
- StemFlex Medium
- StemPro hESC SFM
- Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in Essential 8 Medium
- Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in StemFlex Medium
- Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in mTeSR1 Medium on Geltrex matrix
- Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in StemPro Medium
- Vitronectin, truncated recombinant human (VTN-N)
Mouse protocols
- Culturing human embryonic stem cells on MEF-conditioned medium
- Feeder-dependent culture and passaging mouse embryonic stem cells (mESCs) in KnockOut Serum Replacement with leukemia inhibitory factor (LIF)
- Formation of embryoid bodies (EBs) from mouse embryonic stem cells (mESCs)
- Mouse (ICR) Inactivated Embryonic Fibroblasts
Cell engineering protocols
- Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in Essential 8 Medium
- Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in StemFlex Medium
- Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in mTeSR1 Medium on Geltrex matrix
- Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in StemPro Medium
Cell differentiation protocols
Ectoderm
- Derivation of dopaminergic neurons from human embryonic stem cells
- Differentiating glial precursor cells into astrocytes and oligodendrocytes
- Human Dopaminergic Neuron Immunocytochemistry Kit
- Human Neural Stem Cell Immunocytochemistry Kit
- Induction of neural stem cells from human pluripotent stem cells using Gibco PSC Neural Induction Medium
- PSC Dopaminergic Neuron Differentiation Kit (prototype)
- PSC Neural Induction Medium
- PSC Neural Induction Medium quick reference guide
Mesoderm
- Differentiating neural stem cells into neurons and glial cells
- Enrichment and replating of PSC-derived cardiomyocytes
- Human Cardiomyocyte Immunocytochemistry Kit
- PSC Cardiomyocyte Differentiation Kit
- PSC Cardiomyocyte Differentiation Kit quick reference guide
- Single-cell dissociation of PSCs for cardiomyocyte differentiation
Endoderm
Additional differentiation protocols
Cell reprogramming protocols
- CytoTune EmGFP Sendai Fluorescence Reporter
- CytoTune-iPS 2.0 Sendai Reprogramming Kit
- Epi5 Episomal iPSC Reprogramming Kit
- Episomal iPSC Reprogramming Vectors
- Generation of Human Induced Pluripotent Stem Cells (hiPSC) from Fibroblasts using Episomal Vectors
- Reprogramming peripheral blood mononuclear cells (PBMCs) with the CytoTune-iPS 2.0 Reprogramming Kit under feeder-free conditions
- GeneArt CRISPR Nuclease Vector Kit
Cell culture protocols
- Adaptation of hPSCs from different culture systems to Essential 8 Medium or Essential 8 Flex Medium
- Creation of embryoid bodies from iPSCs using Complete KnockOut Serum Replacement Feeder-Free Medium
- Culturing induced pluripotent stem cells (iPSCs) on MEF-conditioned medium
- Culturing PSCs in Essential 8 Medium
- Essential 6 Medium
- Essential 8 Adaptation Kit
- Essential 8 Flex Medium
- Essential 8 Medium
- Feeder-dependent culture of human embryonic stem cells (hESCs)
- Feeder-dependent culture of human induced pluripotent stem cells (hiPSCs)
- Geltrex LDEV-Free, hESC-qualified, Reduced Growth Factor Basement Membrane Matrix
- Gibco Episomal hiPSC Line
- RevitaCell Supplement
- StemScale PSC Suspension Medium
- StemFlex Medium
- StemPro hESC SFM
- Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in Essential 8 Medium
- Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in StemFlex Medium
- Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in mTeSR1 Medium on Geltrex matrix
- Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in StemPro Medium
- Vitronectin, truncated recombinant human (VTN-N)
Mouse protocols
- Culturing human embryonic stem cells on MEF-conditioned medium
- Feeder-dependent culture and passaging mouse embryonic stem cells (mESCs) in KnockOut Serum Replacement with leukemia inhibitory factor (LIF)
- Formation of embryoid bodies (EBs) from mouse embryonic stem cells (mESCs)
- Mouse (ICR) Inactivated Embryonic Fibroblasts
Cell engineering protocols
- Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in Essential 8 Medium
- Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in StemFlex Medium
- Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in mTeSR1 Medium on Geltrex matrix
- Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in StemPro Medium
Cell differentiation protocols
Ectoderm
- Derivation of dopaminergic neurons from human embryonic stem cells
- Differentiating glial precursor cells into astrocytes and oligodendrocytes
- Human Dopaminergic Neuron Immunocytochemistry Kit
- Human Neural Stem Cell Immunocytochemistry Kit
- Induction of neural stem cells from human pluripotent stem cells using Gibco PSC Neural Induction Medium
- PSC Dopaminergic Neuron Differentiation Kit (prototype)
- PSC Neural Induction Medium
- PSC Neural Induction Medium quick reference guide
Mesoderm
- Differentiating neural stem cells into neurons and glial cells
- Enrichment and replating of PSC-derived cardiomyocytes
- Human Cardiomyocyte Immunocytochemistry Kit
- PSC Cardiomyocyte Differentiation Kit
- PSC Cardiomyocyte Differentiation Kit quick reference guide
- Single-cell dissociation of PSCs for cardiomyocyte differentiation
Endoderm
Additional differentiation protocols
Featured stem cell 'how to' videos
Additional resources
Support
For Research Use Only. Not for use in diagnostic procedures.