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StepOne™ and StepOnePlus™ Real-Time PCR Systems Support
StepOne/StepOne Plus has been discontinued since 30 June 2024. Visit QuantStudio Real-Time PCR Systems for a replacement product. Contact Us for more information.
Explore the “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through the “Guides and Tools” section to access comprehensive product-related support resources.
Getting Started
- When do I need to calibrate the StepOne™ or StepOnePlus™ Real-Time PCR System?
- When do I need to run the RNaseP calibration?
- How do I load tube strips on the StepOne™ or StepOnePlus™ Real-Time PCR System?
- What plates or tubes can I use with the StepOne™ and StepOnePlus™ Real-Time PCR Systems?
- How do I use the VeriFlex™ block for PCR gradients on the StepOnePlus™ Real-Time PCR System?
Troubleshooting
- What does “Error - Failure to acquire needed controlling rights” mean?
- What does “Error - Analysis failed due to GExSession doesn’t exist in context” mean?
- What does “Error - Java.lang.IllegalArgumentException: One of the raw spectra is null” mean?
- What does “Error - Fatal Error Number 0x8158” mean?
- My tubes are melting. What is wrong?
Guides and Tools
- Brochure: StepOne™ and StepOnePlus™ Real-Time PCR Systems
- Brochure: OQ/IPV Service for Real-Time PCR Systems
- Fact Sheet: Green Features: StepOnePlus™ System Comparison to Bio-Rad CFX96™ System
- Guide: Getting Started: Genotyping Experiments on the StepOne™ and StepOnePlus™ Real-Time PCR Systems
- Guide: Getting Started: Standard Curve Experiments on the StepOne™ and StepOnePlus™ Real-Time PCR Systems
- Guide: Getting Started: Relative Standard Curve and Comparative CT Experiments on the StepOne™ and StepOnePlus™ Real-Time PCR Systems
- Guide: Getting Started: Presence/Absence Experiments on the StepOne™ and StepOnePlus™ Real-Time PCR Systems
- Guide: Installation, Networking, and Maintenance User Guide on the StepOne™ and StepOnePlus™ Real-Time PCR Systems
- Guide: Reagent Handbook for the StepOne™ and StepOnePlus™ Real-Time PCR Systems
- Guide: How to Use MicroAmp™ Reaction Plates, Tube Strips, and Tubes
- Selection Table: Real-Time PCR Master Mixes and Instrument Compatibility
- Technical Note: StepOne™ and StepOnePlus™ Specification Sheet
- Search our database for Product Documents: Manuals, Product Literature, Citations and References
- Search our database for Quality and Safety Documents: MSDS, COA
- Overview: Plastic Consumables (legacy Thermo Scientific™)
- General: qPCR Resource Library (legacy Thermo Scientific™)
- Selection: qPCR and PCR Plastics Selection Tool
- Selection Table: Instrument Service Plans
- Selection Table: Real-Time PCR Instrument Spare Parts
- Selection Table: Real-Time PCR System Calibration Kit Selection Guide
- Selection Table: Choosing your PCR Accessories (Tubes, Strips, Plates, etc.)
- Video: StepOne™ and StepOne Plus™ PCR Systems Overview
- Video: Fixing Software Setup Mistakes in the StepOne™ or StepOnePlus™ Software
- Video: OEM Manufacturing Capabilities for Plastic Consumables (legacy Thermo Scientific™)
- Video: Flexible Consumables for PCR & qPCR (legacy Thermo Scientific™)
- Video: Introduction to Thermo Fisher Cloud
- Training: StepOne™ and StepOnePlus™ Instruments Setup and Basic Overview
- Training: StepOne™ and StepOnePlus™ Systems: Software Demonstrations
- Browse our site for: Training, Events, and Webinars
- Software: StepOne™ and StepOnePlus™ Software
- Tool: PCR Tubes, Plates, and Accessories Selection Guide
- Tool: Real-Time PCR Troubleshooting Tool
- Tool: Instrument Service Coverage Lookup - Find warranty and service contract information for your instrument
- Tutorial: Learn How to Set Up an AQ Experiment Using an Interactive StepOne Software Tutorial
- Tutorial: Learn How to Set Up a ddCT Experiment Using an Interactive StepOne Software Tutorial
- Reagent Rewards: An app to turn reagent purchases into rewards
- Real-Time PCR: An app for real-time PCR resources, including videos, an interactive troubleshooting tool, and a reaction calculator
Services
One central location to handle all your Instrument and Services requests.
Custom Services and Reagents for Real-Time PCR (qPCR)
Our Genetic Analysis Custom Products and Services team offers a wide range of choices that include customized reagents such as master mixes or custom-designed TaqMan® assays. We will work with you to develop a custom solution that fits your needs.
Contract Laboratory Services for Real-Time PCR and other Genetic Analysis
Our Custom Products and Services team can handle large projects when you do not have access to all the right equipment or reagents. Our contract research work includes designing and performing experiments including sample preparation, data analysis, and reporting for many different applications.
For Research Use Only. Not for use in diagnostic procedures.