Closed System Compatible Media and Reagents

Gibco CTS AIM V Medium is a fully defined serum-free formulation for proliferation and/or manipulation of T cells, dendritic cells, and other primary or immortalized cells. It contains L-glutamine and is formulated with or without phenol red and antibiotics, making it ideal for research or manufacturing. Gibco CTS AIM-V Medium, without phenol red, without antibiotics, is now accessible with flexibility to sterile weld or aseptically connect to closed systems in a fill volume relevant for autologous and small-scale allogeneic CGT manufacturing.

• Adherence to supplier-related responsibilities of USP <1043>*
• Approved for use in multiple clinical trials globally
• Intuitive design and versatility connection to close your workflow

*Other aspects of USP <1043> will be the responsibility of the end-user to assess. Thermo Fisher Scientific cannot fulfill USP <1043> in regards to application and therapy-specific aspects (e.g., residual assessment and removal of the AM).

Gibco CTS NK-Xpander Medium is an animal origin-free, GMP-manufactured medium designed to proliferate a high yield of functional human NK cells to address the needs of translational and clinical cell therapy researchers​ with or without the use of feeder cells. The 5 L BPC can be sterile welded or aseptically connected to closed systems in a fill volume relevant for autologous NK cell therapy manufacturing.

• Capable of expanding a high yield of functional human NK cells within 14 days without the use of feeder cells
• Animal origin-free, manufactured under GMP, and designed to help meet regulatory compliance requirements for ancillary materials used in cell and gene therapy manufacturing 
• Available in bag format for closed system compatibility with reduced risk of contamination

Gibco Cell Therapy Systems CTS DPBS is the classical Dulbecco’s Phosphate Buffered Saline not containing calcium, magnesium, or phenol red. Gibco CTS DPBS, without calcium chloride, without magnesium chloride is now accessible with flexibility to sterile weld or aseptically connect to closed systems in a fill volume relevant for autologous and small-scale allogeneic CGT manufacturing.

• Provides an environment that maintains the structural and physiological integrity of cells in vitro
• Chemically defined and animal origin-free materials suitable for use in clinical research studies
• Intuitive design and versatility connection to close your system workflow
  
  

Gibco Cell Therapy Systems CTS DPBS, the classical Dulbecco’s Phosphate Buffered Saline containing calcium and magnesium chloride is now available in both a bottle or a flexible BioProcess Container (BPC) suitable for use with closed-system instruments.

• A balanced salt buffer that maintains pH and osmotic balance for washing cells or reagent preparation
• GMP manufactured for use in clinical research studies
• Versatile BPC design that allows for sterile welding, MPC quick connect, or Luer lock connections to integrate into your closed-system workflow

Gibco CTS OpTmizer Pro SFM, bag format is a novel serum-free medium (SFM) without phenol red developed for the growth and expansion of human T lymphocytes (e.g., CD4+, CD8+, polyclonal cells, or antigen-specific T cells) used in allogeneic cell therapies. The medium maintains central memory T cells further into expansion, which is well suited for longer workflows (>15 days). The result is a larger population of central memory T cells in a shorter period of time.

• Maintains central memory T cells further into expansion
• Supports T-cell activation using Dynabeads magnetic beads, soluble antibodies, and stimulatory antibody-presenting cell protocols 
• Ideally suited to the production of allogeneic therapies

CTS Immune Cell Serum Replacement (SR) is designed to support expansion of in vitro cultured human immune cells such as T cells. A defined xeno-free formulation proven for clinical use and designed to support expansion of in vitro cultured human T cells when added as a supplement to a basal cell culture medium such as Gibco CTS OpTmizer T Cell Expansion SFM or AIM V Medium.

• Minimizes the supply and safety risks associated with human serum and can be used with the base media of your choice
• Supports expansion, efficacy and persistence of lentiviral gene-modified CAR T cells
• Supports T cell phenotype (CD4, CD8, and CD62L) similar to human serum
• Available in bioprocess container format, the chamber is constructed from our highest quality Aegis 5-14 film. Sterile, weldable C-Flex tubing with a female Luer lock or MPC quick connect provides versatile connection options for integrating into your closed process.

ICSR also available in 1L format, Large Volume Configuration B

Gibco CTS GlutaMAX supplement is an animal origin–free (AOF) dipeptide alternative to L-glutamine, with increased stability that improves cell health. CTS GlutaMAX supplement can be used as a direct substitute for L-glutamine at equimolar concentrations in mammalian and stem cell culture with minimal or no adaptation.

• Minimizes toxic ammonia build-up—unlike L-glutamine, GlutaMAX Supplement does not spontaneously break down to form ammonia. This prevents waste build-up and maintains a fresh supply of L-glutamine during long-term culture
• Improves growth efficiency and performance of mammalian cell cultures—lower levels of ammonia results in improved cell viability; in addition, culture conditions are more controlled due to the consistent concentration of GlutaMAX supplement that is not possible with L-glutamine
• Remains stable across a wide range of temperatures—CTS GlutaMAX supplement retains full functionality at room temperature or 2–8°C, for convenient storage and handling

Gibco CTS TrypLE Select Enzyme, is a recombinant enzyme capable of dissociating a wide range of adherent mammalian cells. CTS TrypLE Select Enzyme is derived from microbial fermentation and provides an animal origin–free (AOF) alternative for porcine trypsin.

• Animal origin–free (AOF)—free of animal-derived components and formulated on dedicated animal origin-free equipment; CTS TrypLE Select Enzyme may minimize the risk of viral contamination in your manufacturing process
• Room temperature-stable—product remains stable at room temperature or 2–8°C, making storage and handling easy and convenient
• Easy to use—CTS TrypLE Select Enzyme substitutes directly into existing protocols with no inactivation required, avoiding the need for trypsin inhibitors, such as FBS
   

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CTS Immune Cell Serum Replacement (SR) is designed to support expansion of in vitro cultured human immune cells such as T cells. A defined xeno-free formulation proven for clinical use and designed to support expansion of in vitro cultured human T cells when added as a supplement to a basal cell culture medium such as Gibco CTS OpTmizer T Cell Expansion SFM or AIM V Medium.

• Minimizes the supply and safety risks associated with human serum and can be used with the base media of your choice
• Supports expansion, efficacy and persistence of lentiviral gene-modified CAR T cells
• Supports T cell phenotype (CD4, CD8, and CD62L) similar to human serum
• Available in bioprocess container format, the chamber is constructed from our highest quality Aegis 5-14 film. Sterile, weldable C-Flex tubing with a female Luer lock or MPC quick connect provides versatile connection options for integrating into your closed process.

ICSR also available in 250 mL format, Small Volume Configuration