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The production of therapeutic antibodies and proteins is one of the fastest growing sectors of the pharmaceutical industry, where accuracy and consistency in bioprocessing can pose challenges. From the first discovery of insulin to the most recent ground-breaking therapeutics, we lead the way in partnering with biopharmaceutical companies to improve the efficiency and safety in manufacturing and processing of therapeutic antibodies.
Ammonium sulfate precipitation is a method of protein purification by altering the solubility of protein. Ammonium sulfate is commonly used due to its high solubility that allows salt solutions with high ionic strength.
Although stable Chinese Hamster Ovary (CHO) transfectants are routinely used for large-scale production of recombinant protein, TGE in CHO cells has historically provided considerably lower amounts of recombinant material for evaluation. This has prompted the use of TGE in higher yielding human embryonic kidney (HEK293) cells to evaluate target proteins before stable CHO transfectants are made.
Cory Stevenson, VP & General Manager for Thermo Fisher Scientific, speaks to our commitment in setting standards.
Access a targeted collection of scientific application notes, case studies, videos, webinars, and white papers for bioproduction.
Access a targeted collection of scientific application notes, case studies, posters, white papers and more for bioprocessing: