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Gibco CHO Media
Optimize your cell culture performance for superior outcomes
Gibco CHO Media offer high performing options in multiple formats designed to optimize your workflow. We have extended our portfolio to include our newest media formulations: Gibco Efficient-Pro™ Medium and Gibco Efficient-Pro™ Feeds 1 and 2 designed for use with a range of CHO cells. This complete collection provides a range of options for optimal CHO cell culture including High-Intensity Perfusion (HIP) CHO Medium, and Gibco ExpiCHO Stable Production Medium (SPM).
What's new—Gibco Efficient-Pro Medium & Feeds help enhance CHO cell protein quality and productivity
Gibco Efficient-Pro Medium and Gibco Efficient-Pro Feeds 1 and 2 help deliver superior product performance, improved product titer, better protein quality and lot-to-lot consistency, which provides more control and predictability. Each medium is chemically defined, completely scalable and available as Gibco Advanced Granulation Technology (AGT) formats for sustained cell viability. The AGT dry media format enables a simple and scalable reconstitution process. These formats help maximize process efficiency with simplified preparation. Customers with larger-scale operations will benefit from longer bioreactor runs, reduced risk in reconstitution and flexibility to support these CHO cell lines.
Gibco Efficient-Pro™ Medium
- Tested in, and pairs best with feeds for, CHO-S, CHO-K1, and CHO-DG44
- Together cell-specific Efficient-Pro Feeds, helps increase titer and cell viability
Gibco Efficient-Pro™ Feed 1
- Tested in, and pairs best with feeds for CHO-K1
- Together with Efficient-Pro Medium, helps increase titer and cell viability
Gibco Efficient-Pro™ Feed 2
- Tested in, and pairs best with feeds CHO-S and DG44
- Together with Efficient-Pro Medium, helps increase titer and cell viability
CHO cells cultured in Efficient-Pro media demonstrated improved protein production—High titers established across CHO variants and vessel size show increased end-product yield, viable cell density, reduced batch numbers, and lower cost with CHO-K1 and CHO-S cells.
CHO-K1 Cells
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Efficient-Pro Feed 1 showed comparable productivity to the competitor. Efficient-Pro Medium and Feed 1 maintained high viability and lower cell density indicating a higher specific productivity compared to the competitor.
CHO-S cells cultured with Efficient-Pro Medium and Efficient-Pro Feed Supplement 2
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Efficient-Pro Feed 2 resulted in 44% higher protein titer over the competitor. Efficient-Pro Medium and Feed 2 maintained viability above 90% during the 14-day run with comparable cell density to the competitor.
Glycan and charge variant profiles—Improved protein quality established across all CHO variants creating increased end-product yield, reduced purification steps, and improved efficacy in CHO-K1 and CHO-S cells.
CHO-K1 charge variant profile
Charge variant profile of CHO-K1 cells. Use of Efficient-Pro Medium and Feed 1 resulted in a preferred charge variant profile with a more neutral profile than competitor medium and feed.
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CHO-S charge variant profile
Charge variant profile of CHO-S cells. Use of Efficient-Pro Medium and Feed 2 resulted in a preferred charge variant profile with a more neutral profile than competitor medium and feed.
Click image to enlarge
High specific productivity of IgG across nearly all CHO variants and vessel size demonstrates opportunity for improved yield, enhanced protein production, and extended harvest stage in CHO-K1, CHO-S, and DG-44 cells.
CHO-K1 cell growth
Efficient-Pro Medium and Efficient-Pro Feed 1 maintained high viability and lower cell density indicating a higher specific productivity compared to the competitor.
CHO-S cell productivity
Compared to the alternative medium and feeds, Efficient-Pro Medium and Feed 2 with CHO-S cells realized with a 144% relative average titer by day 14 and up to 180% relative average specific productivity (qP).
DG-44 cell productivity
Compared to the alternative medium and feeds, Efficient-Pro Medium and Feed 2 with DG44 cells showed a 312% relative average titer by day 14 with up to a 160% relative average specific productivity (qP).
Click image to enlarge
Click image to enlarge
Click image to enlarge
CHO cells cultured in Efficient-Pro media demonstrated improved protein production—High titers established across CHO variants and vessel size show increased end-product yield, viable cell density, reduced batch numbers, and lower cost with CHO-K1 and CHO-S cells.
CHO-K1 Cells
Click image to enlarge
Efficient-Pro Feed 1 showed comparable productivity to the competitor. Efficient-Pro Medium and Feed 1 maintained high viability and lower cell density indicating a higher specific productivity compared to the competitor.
CHO-S cells cultured with Efficient-Pro Medium and Efficient-Pro Feed Supplement 2
Click image to enlarge
Efficient-Pro Feed 2 resulted in 44% higher protein titer over the competitor. Efficient-Pro Medium and Feed 2 maintained viability above 90% during the 14-day run with comparable cell density to the competitor.
Glycan and charge variant profiles—Improved protein quality established across all CHO variants creating increased end-product yield, reduced purification steps, and improved efficacy in CHO-K1 and CHO-S cells.
CHO-K1 charge variant profile
Charge variant profile of CHO-K1 cells. Use of Efficient-Pro Medium and Feed 1 resulted in a preferred charge variant profile with a more neutral profile than competitor medium and feed.
Click image to enlarge
CHO-S charge variant profile
Charge variant profile of CHO-S cells. Use of Efficient-Pro Medium and Feed 2 resulted in a preferred charge variant profile with a more neutral profile than competitor medium and feed.
Click image to enlarge
High specific productivity of IgG across nearly all CHO variants and vessel size demonstrates opportunity for improved yield, enhanced protein production, and extended harvest stage in CHO-K1, CHO-S, and DG-44 cells.
CHO-K1 cell growth
Efficient-Pro Medium and Efficient-Pro Feed 1 maintained high viability and lower cell density indicating a higher specific productivity compared to the competitor.
CHO-S cell productivity
Compared to the alternative medium and feeds, Efficient-Pro Medium and Feed 2 with CHO-S cells realized with a 144% relative average titer by day 14 and up to 180% relative average specific productivity (qP).
DG-44 cell productivity
Compared to the alternative medium and feeds, Efficient-Pro Medium and Feed 2 with DG44 cells showed a 312% relative average titer by day 14 with up to a 160% relative average specific productivity (qP).
Click image to enlarge
Click image to enlarge
Click image to enlarge
Learn more about AGT dry media formats and their advantages
Learn more about available media formats designed to support optimal CHO cell culture
High-Intensity Perfusion CHO Medium
Gibco High-Intensity Perfusion (HIP) CHO Medium can be used at multiple concentrations to support your process from seed train to production.
Just as it can be used at multiple concentrations, it can be used in multiple perfusion processes: N-1 and seed train, intensified fed-batch, concentrated fed batch, and continuous perfusion. Numerous CHO cell lines (CHO-S, CHO-DG44, CHO-GS, CHO-K1) have been tested in these perfusion processes with HIP CHO Medium.
In addition, the medium supports high cell densities at low medium exchange rates, which improves process efficiency, as low as 1 vessel volume/day (VVD).
Key features of HIP CHO Medium
Features | Benefits |
Advanced granulated technology (AGT) format | Easy to use |
Works with numerous CHO clones | CHO-S, CHO-GS, CHO-K1, CHO-DG44 |
Works in a variety of processes | Intensified fed-batch, concentrated fed batch, N-1, and continuous perfusion |
Supports high cell densities at low medium exchange rates | Improved process efficiency |
Supports multiple reconstituted concentrations | Product can be flexibly reconstituted to meet process needs |
Gibco ExpiCHO SPM
Gibco ExpiCHO SPM eliminates guesswork needed to find compatible media and feeds for scale-up. Simply transition your ExpiCHO-S cells after stable transfection for the same high titer and virtually none of the optimization.
- Seamless transition
- Confidence in performance
- Risk reduction
Ideal with:
Cells | ExpiCHO-S |
Feeds | EfficientFeed C+ |
Protein Quality | GlycanTune Total Feeds |
ExpiCHO family includes
- ExpiCHO expression system for transient expression
- ExpiCHO-S cells
- ExpiCHO SPM
All products are designed to work seamlessly with one another easing the transition to stable expression helping you to commercialization faster.
Provides you with:
- ONE solution—no media optimization required
- Hassle-free scale-up—stable clone development protocol available
The ExpiCHO SPM formulation is optimized for the ExpiCHO-S cells and outperforms other CHO medium on the market.
Figure 1 illustrates a comparative analysis. Stable clones were developed using the ExpiCHO stable clone development protocol and then transitioned to test media for an Ambr15 run. The highest output was achieved by ExpiCHO SPM, with the liquid and AGT (dry) formats performing comparably. The data represents 16 replicates for each format of ExpiCHO SPM and 3 replicates each for ActiPro and Advanced CHO respectively.
Figure 1. Comparative performance of ExpiCHO SPM and other commercial media in ExpiCHO-S cells.
Efficient-Pro Medium and Feeds System
The Gibco Efficient-Pro Medium and Feed System is designed to drive productivity in your batch or fed-batch CHO process. This system includes state-of-the-art basal medium and carefully optimized single-part feeds designed specifically for our cell line.
High-Intensity Perfusion CHO Medium
Gibco High-Intensity Perfusion (HIP) CHO Medium can be used at multiple concentrations to support your process from seed train to production.
Just as it can be used at multiple concentrations, it can be used in multiple perfusion processes: N-1 and seed train, intensified fed-batch, concentrated fed batch, and continuous perfusion. Numerous CHO cell lines (CHO-S, CHO-DG44, CHO-GS, CHO-K1) have been tested in these perfusion processes with HIP CHO Medium.
In addition, the medium supports high cell densities at low medium exchange rates, which improves process efficiency, as low as 1 vessel volume/day (VVD).
Key features of HIP CHO Medium
Features | Benefits |
Advanced granulated technology (AGT) format | Easy to use |
Works with numerous CHO clones | CHO-S, CHO-GS, CHO-K1, CHO-DG44 |
Works in a variety of processes | Intensified fed-batch, concentrated fed batch, N-1, and continuous perfusion |
Supports high cell densities at low medium exchange rates | Improved process efficiency |
Supports multiple reconstituted concentrations | Product can be flexibly reconstituted to meet process needs |
Gibco ExpiCHO SPM
Gibco ExpiCHO SPM eliminates guesswork needed to find compatible media and feeds for scale-up. Simply transition your ExpiCHO-S cells after stable transfection for the same high titer and virtually none of the optimization.
- Seamless transition
- Confidence in performance
- Risk reduction
Ideal with:
Cells | ExpiCHO-S |
Feeds | EfficientFeed C+ |
Protein Quality | GlycanTune Total Feeds |
ExpiCHO family includes
- ExpiCHO expression system for transient expression
- ExpiCHO-S cells
- ExpiCHO SPM
All products are designed to work seamlessly with one another easing the transition to stable expression helping you to commercialization faster.
Provides you with:
- ONE solution—no media optimization required
- Hassle-free scale-up—stable clone development protocol available
The ExpiCHO SPM formulation is optimized for the ExpiCHO-S cells and outperforms other CHO medium on the market.
Figure 1 illustrates a comparative analysis. Stable clones were developed using the ExpiCHO stable clone development protocol and then transitioned to test media for an Ambr15 run. The highest output was achieved by ExpiCHO SPM, with the liquid and AGT (dry) formats performing comparably. The data represents 16 replicates for each format of ExpiCHO SPM and 3 replicates each for ActiPro and Advanced CHO respectively.
Figure 1. Comparative performance of ExpiCHO SPM and other commercial media in ExpiCHO-S cells.
Efficient-Pro Medium and Feeds System
The Gibco Efficient-Pro Medium and Feed System is designed to drive productivity in your batch or fed-batch CHO process. This system includes state-of-the-art basal medium and carefully optimized single-part feeds designed specifically for our cell line.
Gibco media and feed selections by cell line
This table provides information on the most likely candidate based on a specific cell line. As all clones are unique, other media may in fact work more ideally and it is suggested to try a variety of media to test.
For more information on considerations when selecting the optimal match, go to our next section.
| CHO cell type | Gibco media option | Matched feed option | Does medium contain growth factor? (Y/N) | Is adaption necessary? (Y/N) |
|---|---|---|---|---|
| CHO-S | Efficient-Pro Medium | Efficient-Pro Feed 2 | N | Y |
CHO Medium | Difco TC Yeastolate UF | Y | Y | |
Dynamis Medium | EfficientFeed C+ | N | Y | |
ExpiCHO-S | ExpiCHO SPM w/gf add back | EfficientFeed C+ | Y | Y |
| ExpiCHO SPM | EfficientFeed C+ | N | N |
DG44/DXB11 | Efficient-Pro Medium | Efficient-Pro Feed 2 | N | Y |
| CHO Medium | OneFeed Supplement | Y | Y |
| Dynamis or CD OptiCHO Medium | EfficientFeed C+ or EfficientFeed A+ | N | N |
CHO-K1 | Efficient-Pro Medium | Efficient-Pro Feed 1 | N | Y |
Dynamis Medium | EfficientFeed C+ | N | Y | |
GS CHO | CD-1000 Medium | Resurge CD5 Supplement | Y | Y |
| Dynamis or CD CHO | EfficientFeed C+ or EfficientFeed B+ | N | Y |
Other CHO | CHO Medium | Difco TC Yeastolate UF | Y | Y |
Dynamis or CD CHO Medium | EfficientFeed B+ or EfficientFeed C+ | N | Y |
*Drug Master File (DMF) information may be available and can be found at Drug Master Files (DMFs) and Regulatory Support Files (RSFs).
Find your optimal match with Gibco cell culture media
There are several pathways to find the right media or media and feed solution for CHO cell cultures. These pathways include selecting a standard or off-the-shelf media, our CHO media test panel, or a media development or optimization service. In order to find the right path, we need to remove the biggest obstacle; luck.
Often time, your goals on what you want to accomplish will narrow down the best selections for you.
Questions to consider:
- What are you hoping to accomplish?
- Do you know your timeline?
- What is most critical?
Comparison of the different options available
Standard catalog media | Media test panel | Development or optimization service | |
Definition | Standard catalog products include our off-the-shelf CHO media, feed and supplement options. | Developed media panels with diverse formulations offering improved specificity, without the need for a full media development service. | Service solutions are provided by our Gibco BioProduction Services team, offering cell line development, media development, media optimization, process optimization, consultations, prototyping, format conversions and more. |
Time | Fast | Relative speed | Dependent on scope; >3 months |
Budget | Economical | Mostly cost-effective | More investment needed |
Titer | Average to meets requirements | Meets requirements | Meets or surpasses requirements |
Benefits | Quick turn-around Standard media are available off-the-shelf. Easy scale-up Liquid and dry formats available that provide performance consistency and may reduce workflow steps to simplify your process. Risk mitigation and assurance of supply May be produced at either of our harmonized manufacturing locations. Available data Frequently, used in application notes and further testing. | Capacity Secondary sourcing options; easy scale-up More specificity Options that are more specific to your clone and molecule. Relative speed Speed is important but not as much as matching product quality. Regulatory support For the panels, provides regularity support needed to succeed in getting approvals. | Resources and/or expertise Need to outsource or consultation on your process Secondary sourcing Increase capacity of your own media and mitigate risk Scale-up solutions Transitioning from a liquid to a dry format during scale-up or performance support during scale-up or tech transfer Improved performance Optimal solution that delivers on your performance criteria Platform media Options that work better than current platform for multiple molecules Regulatory support We offer royalty-free CHO cell lines and documentation for regulatory filings. |
Frequently, cell line specific component dependencies, like insulin and other growth factors, are missed when evaluating media. These are components that are needed for your cell line to thrive (growth, productivity) and if they are not present in the tested media, you may miss out on the optimal solution.
Matching media to your cell line should include an evaluation of whether these dependencies are in the media of choice or not (Tables 1 and 2) to determine whether you need to add them in or not.
Even with the cell line dependency component, in most cases, adaptation is required to see optimal results.
Additional components may also need to be considered for an optimal outcome (Table 3).
Questions:
- Do you know if your cell line has any dependencies?
- Are you planning on adapting the cells to the media?
- Are other considerations necessary for the best result?
Standard catalog media | Media test panel | Development or optimization service | |
Dependencies | Table 1 lists media containing growth factors Table 2 lists media without growth factors | Our media test panel does not contain typical cell line dependencies by default and can be added in as requested. | Our service offerings will evaluate these factors and other factors as specified. |
Considerations | Additional components may be necessary for optimal performance (Table 3). | ||
Table 1. Products that contain a growth factor, by cell line.
Cell type | Media option | Matched feed option |
CHO-S | CHO Medium | Difco TC Yeastolate UF |
ExpiCHO-S | ExpiCHO SPM* w/gf add back | EfficientFeed C+ |
DG44/DXB11 | NutriCHO CD Medium* EX, LX, MAX | OneFeed Supplement |
GS CHO | CD-1000 Medium | Resurge CD5 Supplement |
Other CHO | CHO Medium | Difco TC Yeastolate UF |
ALL | Development or optimization Services and Media Test Panel | Based on specifications |
* No cell adaptation required
Table 2. Products that do not contain a growth factor, by cell line.
Cell type | Media option | Matched feed option |
CHO-S | Dynamis Medium | EfficientFeed C+ Supplement |
ExpiCHO-S | ExpiCHO SPM* | EfficientFeed C+ Supplement |
DG44/DXB11 | CD OptiCHO Medium* | EfficientFeed A+ Supplement |
CHO-K1 | Dynamis Medium | EfficientFeed C+ Supplement |
GS CHO | Dynamis Medium CD CHO Medium | EfficientFeed C+ Supplement EfficientFeed B+ Supplement |
Other CHO | Dynamis Medium | EfficientFeed C+ Supplement |
All | Development and optimization services and Media Test Panel | Based on specifications |
* No cell adaptation required
Table 3. Troubleshooting components to bundle with your medium selection.
Situation | Recommendation |
Cell Line cloned in medium containing insulin | Insulin and/or Long R3 IGF |
Cell line is clumping | Titrate Anti-Clumping Agent starting at 1% |
Cell line is sensitive to ammonia | |
Cell line is not sensitive to ammonia | |
Performance drops (productivity, apoptosis), especially during scaling up | |
Need to adjust the glycosylation profile | GlycanTune A+, B+ or C+ Total Feeds or GlycanMod Pak |
Not all CHO clones are alike; each clone has a different metabolic baseline. When determining a new medium for your clone, you may want to consider a variety of available medium on the market to test. All options are scalable from small- to large-scale in a variety of formats.
Standard catalog media | Media test panel | Development or optimization service | |
Options | Order samples based on the below groupings for a diverse panel: No growth factors in medium
Containing growth factors in medium
| Panel consists of five diverse chemically defined media which can be mixed to produce 21 test conditions
| In partnership with you, this service will provide solutions that are specific to your requirements. |
CHO media—ordering information
The flexibility of Gibco CHO media and feeds give you options to pair with most industry utilized cell lines, and are available in both catalog and customized options in multiple formats to suit your application needs:
- Faster growth
- Highest performing titers
- Sustained viable cell density
- Chemically defined, serum-free, protein-free, and animal origin-free (AOF)
- Flexible formats: liquid and AGT
- Options to customize for every medium
Related products and services
With EfficientFeed A+, B+, and C+ feeding supplements, deliver super-concentrated nutrients in a complete, easy-to-prepare, dry-format feed.
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