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Easily track and go back
Catalog number: AMF5000SV
The EVOS M5000 microscope is exceptional for cell imaging labs across a broad range of applications and imaging requirements. With its powerful software tools, the EVOS M5000 helps revolutionize your imaging workflow.
The Stage View feature allows you to track your location as you manually move the stage and search for your cells. Areas of interest can be marked, saved, and returned to, at any time. Designed for biologists by biologists, the EVOS M5000 with Stage View is remarkably easy to use.
New stage-tracking intelligence
Watch this video to see how you can effortlessly locate, track, and record points of interest.
Features
In addition to the common features of all EVOS Microscopes, the EVOS M5000 offers:
Sample location tracking intelligence
Drop pins to simplify returning to precise stage locations with proprietary EVOS Stage View software
Easy self-installation
No maintenance, assembly, alignment, or calibration
Advanced color options
User-defined 4-color fluorescence, transmitted light, and color images
Thermo Fisher Connect™ connectivity
Images can be uploaded directly from the EVOS M5000 instrument to a Thermo Fisher Connect account. Any new software upgrade can also be downloaded directly in connected instruments. Additional post-acquisition image analysis can be performed via the EVOS Image Analysis application on Thermo Fisher Connect.
Optimal Performance
Autofocus, Z-stack capability, time-lapse imaging, and single-click multichannel capture
Biosafety cabinet compatible
Can be used within a biosafety cabinet or hood
EVOS Onstage Incubator
Compatibility for live-cell analysis with precise control of temperature, humidity, and gases
Automation
Automated cell counting and confluence measurements in the onboard software
Enhanced ease of use
Most operational functions can be controlled through software, helping eliminate the need to touch the instrument except to mount the slide and adjust the stage
Celleste Image Analysis Software option
Compatibility for advanced image processing and analysis, including 2D and 3D deconvolution and 3D visualization and analysis
Location intelligence
The proprietary EVOS Stage View feature allows you to track your location as you manually move the stage and search for your cells. Areas of interest can be pinned, saved and returned to, at any time. Sets of pins can be saved as a map so you can power off the instrument and return to the saved locations later. The software lets you load a saved map and easily re-locate areas of interest in your sample.
.jpg "results from location intelligence study")
Easily return to the same cells with a manual microscope using location intelligence of the Stage View feature of the EVOS M5000 Imaging System. U2OS cells incubated with Invitrogen CellLight Tubulin-GFP, BacMam 2.0 (Cat. No. C10613) and CellLight Nucleus-RFP, BacMam 2.0 (Cat. No. C10603) were treated with 1 mM paclitaxel. Multiple pins were placed using Stage View at time 0; cells located at pin 9 were easily located and imaged at 2.5, 5, and 10 hours.
Demo videos & customer stories
Featured Images
Specifications
| Category | Attribute | AMF5000SV* |
| Optics | Description | Infinity-corrected optical system; RMS threaded objectives with 45 mm parfocal distance |
| Imaging mode | Fluorescence, brightfield, color brightfield, phase contrast | |
| Imaging methods | Single color, multicolor, time lapse, Z-stacking, movie capture | |
| Illumination | Adjustable-intensity LED light cubes with integrated hard-coated filter set and >50,000-hour life | |
| Light cube capacity | 4-position chamber, for 4 fluorescence cubes + brightfield | |
| Light cubes (not included) | Broad selection of standard and specialty light cubes. Commonly used light cubes include (ex/em): • DAPI (357/447 nm) • GFP (470/525 nm) • RFP (531/593 nm) • Texas Red (585/624 nm) • Cy5 (628/692 nm) |
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| Objective capacity | 5-position manual turret, not automated | |
| Objectives (not included) | Wide selection of high-quality long working distance (LWD) and coverslip-corrected objectives | |
| Condenser | 60-mm LWD condenser; 4-position turret with a clear aperture and 3 phase annuli | |
| Focus mechanism | Automated focus with sub-micron (0.150 µm) resolution (single-step accuracy) | |
| Monochrome camera | High-sensitivity 3.2 MP (2048 x 1536) CMOS sensor with 3.45 µm pixel resolution | |
| Captured images | 16-bit RAW monochrome: TIFF, PNG (12-bit dynamic range) 8-bit color: TIFF, PNG, JPG Movies and time-lapse: AVI, WMV |
|
| Physical characteristics | Power supply | 24V AC adapter with country-specific power cords** |
| Dimensions (W x H x D) | 18 x 23 x 18 inches 45.7 x 58.4 x 45.7 cm |
|
| Weight | 36 lb (16.4 kg) | |
| Stage | X/Y stage | Travel range 120 mm x 80 mm with sub-micron resolution Drop-in inserts to receive vessel holders |
| Stage mechanism | Mechanical | |
| Stage tracking | Proprietary functionality to track stage locations in the EVOS Stage View software | |
| System | Computer | Embedded PC with 4 GB RAM |
| Storage | 10 GB SSD 16 GB USB 3.0 memory stick |
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| Output ports | Power; 4 x USB 2.0; 1 x USB 3.0; 1 Display Port; 1 RJ45 | |
| Networking capability | Connection through Windows/SMB network via Ethernet cable connection USB 3.0 WiFi dongle |
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*AMF5000SV is now available for pilot sales in selected regions.
**In regions not using the Type B (North America) power plug, one (1) country-specific power cord must be ordered separately:
AMEP4646 EVOS Power Cord, Type C (Europe) AMEP4708 EVOS Power Cord, Type D (India/South Africa) AMEP4645 EVOS Power Cord, Type G (United Kingdom) AMEP4649 EVOS Power Cord, Type H (Israel) AMEP4647 EVOS Power Cord, Type I (Australia) AMEP4782 Power Cord, Type I (China, CCC certified) AMEP4786 Power Cord Type J (Switzerland) AMEP4648 EVOS Power Cord, Type L (Italy) |
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For Research Use Only. Not for use in diagnostic procedures.