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Amplex Red reagent is a colorless substrate that provides exquisite sensitivity in enzyme-coupled reactions to detect a diverse assortment of analytes, including glucose, galactose, cholesterol, glutamic acid, xanthine (or hypoxanthine), uric acid, choline and acetylcholine, as well as hydrogen peroxide.
Assays using Amplex Red as the substrate result in an increase in fluorescence which is easy to detect in a microplate format. Because the emission is red, there is little interference from autofluorescence in typical cellular samples and the signal shows little sensitivity to pH or reducing agents.
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We've developed Amplex Red reagent–based assays for the detection of key metabolites including glucose/glucose oxidase, galactose/galactose oxidase, and uric acid/uricase assays.
Separate Amplex Red assay kits are available for continuously monitoring AChE activity or for detecting ACh; for continuous measurement of amine oxidase activity in tissue homogenates or purified preparations; and for continuously detecting glutamic acid or for monitoring glutamate oxidase activity.
Assays reported using the sensitive Amplex Red reagent have been developed for the continuous assay of neuraminidase-catalyzed hydrolysis of fetuin, for detecting xanthine or hypoxanthine (or for monitoring xanthine oxidase activity), for measuring catalase activity, and for measuring sphingomyelinase activity in solution.
Amplex Red Galactose/Galactose Oxidase Assay Kit | Amplex Red Glucose/Glucose Oxidase Assay Kit | Amplex Red Uric Acid/Uricase Assay Kit | |
---|---|---|---|
Target | Galactose/Galactose Oxidase | Glucose/Glucose Oxidase | Uric acid/Uricase |
Reporter | Amplex Red reagent | Amplex Red reagent | Amplex Red reagent |
Ex/Em (nm) | 571/585 | 571/585 | 571/585 |
Live cell | No | No | No |
Lysate | Yes | Yes | Yes |
Purified enzyme | Yes | Yes | Yes |
Usage | Lower detection limit 2 x 10–3 U/mL | Lower detection limit 5 x 10–5 U/mL | Lower detection limit 2 x 10–4 U/mL |
Components | Includes assay buffers and controls | Includes assay buffers and controls | Includes assay buffers and controls |
Format | 400 assays using 100 µL reaction volume | 500 assays using 100 µL reaction volume | 400 assays using 100 µL reaction volume |
Protocol outline |
| ||
Cat. No. | A22179 | A22189 | A22181 |
Amplex Acetylcholine/ Acetylcholinesterase Assay Kit | Amplex Red Monoamine Oxidase Assay Kit | Amplex Red Glutamic Acid/Glutamate Oxidase Assay Kit | |
---|---|---|---|
Target | Acetylcholinesterase | Monoamine oxidase | Glutamic Acid/Glutamate Oxidase |
Reporter | Amplex Red reagent | Amplex Red reagent | Amplex Red reagent |
Ex/Em (nm) | 571/585 | 571/585 | 571/585 |
Live cell | No | No | No |
Lysate | Yes | Yes | Yes |
Purified enzyme | Yes | Yes | Yes |
Usage | Ultrasensitive method for continuously monitoring acetylcholinesterase (AChE) activity | Lower detection limit 12 x 10–6 U/mL | Lower detection limit 4 x 10–5 U/mL |
Components | Includes assay buffers and controls | Includes assay buffers and controls | Includes assay buffers and controls |
Format | 500 assays using 200 µL reaction volume | 500 assays using 100 µL reaction volume | 200 assays using 100 µL reaction volume |
Protocol outline |
| ||
Cat. No. | A12217 | A12214 | A12221 |
Amplex Red Neuraminidase (Sialidase) Assay Kit | Amplex Red Xanthine/Xanthine Oxidase Assay Kit | Amplex Red Catalase Assay Kit | Amplex Red Sphingomyelinase Assay Kit | |
---|---|---|---|---|
Target | Neuraminidase (sialidase) | Xanthine/Xanthine oxidase | Catalase | Sphingomyelinase |
Reporter | Amplex Red reagent | Amplex Red reagent | Amplex Red reagent | Amplex Red reagent |
Ex/Em (nm) | 571/585 | 571/585 | 571/585 | 571/585 |
Live cell | No | No | No | No |
Lysate | Yes | Yes | Yes | Yes |
Purified enzyme | Yes | Yes | Yes | Yes |
Usage | Lower detection limit 2 x 10–4 U/mL | Lower detection limit 1 x 10–4 U/mL | Lower detection limit 5 x 10–4 U/mL | Lower detection limit 8 x 10–7 U/mL |
Components | Includes assay buffers and controls | Includes assay buffers and controls | Includes assay buffers and controls | Includes assay buffers and controls |
Format | 400 assays using 100 µL reaction volume | 400 assays using 100 µL reaction volume | 400 assays using 100 µL reaction volume | 500 assays using 200 µL reaction volume |
Protocol outline |
| |||
Cat. No. | A22178 | A22182 | A22180 | A12220 |
Amplex Red Galactose/Galactose Oxidase Assay Kit | Amplex Red Glucose/Glucose Oxidase Assay Kit | Amplex Red Uric Acid/Uricase Assay Kit | |
---|---|---|---|
Target | Galactose/Galactose Oxidase | Glucose/Glucose Oxidase | Uric acid/Uricase |
Reporter | Amplex Red reagent | Amplex Red reagent | Amplex Red reagent |
Ex/Em (nm) | 571/585 | 571/585 | 571/585 |
Live cell | No | No | No |
Lysate | Yes | Yes | Yes |
Purified enzyme | Yes | Yes | Yes |
Usage | Lower detection limit 2 x 10–3 U/mL | Lower detection limit 5 x 10–5 U/mL | Lower detection limit 2 x 10–4 U/mL |
Components | Includes assay buffers and controls | Includes assay buffers and controls | Includes assay buffers and controls |
Format | 400 assays using 100 µL reaction volume | 500 assays using 100 µL reaction volume | 400 assays using 100 µL reaction volume |
Protocol outline |
| ||
Cat. No. | A22179 | A22189 | A22181 |
Amplex Acetylcholine/ Acetylcholinesterase Assay Kit | Amplex Red Monoamine Oxidase Assay Kit | Amplex Red Glutamic Acid/Glutamate Oxidase Assay Kit | |
---|---|---|---|
Target | Acetylcholinesterase | Monoamine oxidase | Glutamic Acid/Glutamate Oxidase |
Reporter | Amplex Red reagent | Amplex Red reagent | Amplex Red reagent |
Ex/Em (nm) | 571/585 | 571/585 | 571/585 |
Live cell | No | No | No |
Lysate | Yes | Yes | Yes |
Purified enzyme | Yes | Yes | Yes |
Usage | Ultrasensitive method for continuously monitoring acetylcholinesterase (AChE) activity | Lower detection limit 12 x 10–6 U/mL | Lower detection limit 4 x 10–5 U/mL |
Components | Includes assay buffers and controls | Includes assay buffers and controls | Includes assay buffers and controls |
Format | 500 assays using 200 µL reaction volume | 500 assays using 100 µL reaction volume | 200 assays using 100 µL reaction volume |
Protocol outline |
| ||
Cat. No. | A12217 | A12214 | A12221 |
Amplex Red Neuraminidase (Sialidase) Assay Kit | Amplex Red Xanthine/Xanthine Oxidase Assay Kit | Amplex Red Catalase Assay Kit | Amplex Red Sphingomyelinase Assay Kit | |
---|---|---|---|---|
Target | Neuraminidase (sialidase) | Xanthine/Xanthine oxidase | Catalase | Sphingomyelinase |
Reporter | Amplex Red reagent | Amplex Red reagent | Amplex Red reagent | Amplex Red reagent |
Ex/Em (nm) | 571/585 | 571/585 | 571/585 | 571/585 |
Live cell | No | No | No | No |
Lysate | Yes | Yes | Yes | Yes |
Purified enzyme | Yes | Yes | Yes | Yes |
Usage | Lower detection limit 2 x 10–4 U/mL | Lower detection limit 1 x 10–4 U/mL | Lower detection limit 5 x 10–4 U/mL | Lower detection limit 8 x 10–7 U/mL |
Components | Includes assay buffers and controls | Includes assay buffers and controls | Includes assay buffers and controls | Includes assay buffers and controls |
Format | 400 assays using 100 µL reaction volume | 400 assays using 100 µL reaction volume | 400 assays using 100 µL reaction volume | 500 assays using 200 µL reaction volume |
Protocol outline |
| |||
Cat. No. | A22178 | A22182 | A22180 | A12220 |
High-sensitivity fluorescence detection for 6-1,536 samples can be quickly performed on the Varioskan ALF or Varioskan LUX Multimode Microplate Reader using Invitrogen reagents to enable optimal detection. Take advantage of automatic dynamic range selection to get optimal gain settings for each individual well and automation capabilities for even higher throughput.
For Research Use Only. Not for use in diagnostic procedures.