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Cell Transfection: High Efficiency Solutions |
Transfection (i.e., delivery) of genes, RNA, or proteins into cells is a foundational technique in the life sciences. Successful transfection is critical for a range of applications, including studies of gene expression, gene knockdown (i.e., RNAi), genome editing, and protein expression for biopharmaceuticals. Furthermore, advances in mammalian cell transfection technologies, such as in vivo delivery, have proven invaluable to clinical research, with these techniques playing pivotal roles in the development of vaccines as well as cell and gene therapies.
Find products, citations, and protocols optimized for your transfection experiments. Input information on your experiment type, cell line, and payload to unlock solutions.
Thermo Fisher Scientific is a trusted supplier of products for highly effective cell transfection. The family of lipid-based Invitrogen Lipofectamine transfection reagents is the most cited line of transfection reagents in the world, with a wide range of products designed for highly effective and efficient nucleic acid delivery into a multitude of cell types. Similarly, the Invitrogen Neon Transfection System has been shown to successfully electroporate over 140 different cell types, offering an efficient, flexible, and simple solution for difficult-to-transfect cells. Select from this range of products built to support in vitro transfection of cells in your lab. If you are seeking solutions for in vivo delivery, discover Invitrogen reagents optimized for use in live organisms (see Invivofectamine 3.0 Reagent).
Invitrogen Lipofectamine products are widely used transfection reagents that provide consistent and highly efficient gene delivery in many cell types. Choose from a large selection of Lipofectamine reagents designed to accommodate a range of applications, from in vitro DNA and RNA delivery to in vivo transfection and gene editing.
Need to learn more about the basics of cationic lipid transfection?
Explore cationic lipid transfection
The Neon NxT Electroporation System is an innovative electroporation device able to achieve high efficiency transfection and increased cell viability, even in hard-to-transfect cell types.
Need to learn more about the basics of electroporation?
Learn about the electroporation method
Transfection can be done using chemical, physical, or biological methodologies. Learn about transfection methods, including factors for selecting the transfection reagent or system that is right for your cell type and payload.
Cell transfection is a fundamental step in a multitude of experimental applications. Whether you are performing academic research to answer core cellular question, expanding into clinical research, or manufacturing recombinant proteins for biopharmaceutical or biotechnological industries, highly efficient cell transfection is critical. Discover an expansive range of transfection applications to fit your experimental needs.
From in vivo delivery of mRNA for vaccine development to transfection of CRISPR-Cas9 for gene editing, find answers and information on performing specific transfection applications in your lab.
Whether you are a student, advanced academic researcher, or looking to scale your processes for clinical or industrial purposes, Thermo Fisher Scientific has the resources you need to succeed. This carefully curated resource collection includes learning centers, an extensive FAQ database, and a large library of product guides and application notes for your cell type and payload.
Explore these featured tools and resources to help select the right transfection solution for your lab and experimental needs.
Packaging must protect the product inside—but that doesn’t have to mean sacrificing sustainability. Lipofectamine products are shipped at ambient temperature, diverting 24,000 ft3 of Expanded Polystyrene (EPS) coolers from landfills annually. We reduce the carbon footprint from packaging and shipping these products by 80 tons (CO2 equivalents).
We've made it easier for you to quickly find and buy products for every step in your transfection workflow.
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For Research Use Only. Not for use in diagnostic procedures.