SARS-CoV-2

Standardize your SARS-CoV-2 RNA extraction method

Achieve faster, more consistent purification of SARS-CoV-2 viral RNA from your samples with Applied Biosystems viral RNA extraction kits. Our kits allow you to efficiently extract viral RNA from sources with a low abundance of nucleic acid. Purified viral RNA is compatible with most downstream assays, including RT-PCR, RT-qPCR, and NGS.

SP SARS workflow

Partner with Thermo Fisher Scientific to go from sample to results. Our technical teams provide comprehensive onsite or remote support so that you can keep the lab up and running.
 

Viral RNA extraction kits optimized for SARS-CoV-2

MagMAX magnetic bead-based kits offer flexible, highly efficient methods for purifying nucleic acids. The following products are specifically recommended for SARS-CoV-2 viral RNA extraction.

 MagMAX Microbiome Ultra Nucleic Acid Isolation Kits

  MagMAX Viral/Pathogen Nucleic Acid Isolation Kit
MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (MVP I)MagMAX Viral/Pathogen Nucleic Acid Isolation Kit MagMAX Bulk Reagents for Viral RNA extraction
  MagMAX Bulk Reagents for Viral RNA extraction product image
MagMAX Prime Viral/Pathogen NA Isolation kit  Viral/Pathogen NA Isolation Kit
Sample typesStool, swabs, transport media, culture media, urine, saliva, and soilUniversal viral transport media (VTM), bronchoalveolar lavage (BAL) and other respiratory research samples, urine, whole blood, and salivaSwabs in transport media (respiratory), saliva, urine
Best forSolid samples and wastewaterLarge sample volumesHighly customized protocolsRunning various sample types on a single plate
Cat. No.A42357 (100 preps), with bead plate
A42358 (100 preps), with bead plate
A48310 (up to 2,000 preps/kit)
A42352 (up to 200 preps/kit)
A42359 (binding solution)
A42360 (wash solution)
A42362 (binding beads)
A42363 (proteinase K)
A42364 (elution buffer)
A58145 (up to 600 preps/kit)
A58147 (binding beats)
A58148 (elution buffer)
A58149 (wash I solution)
A58150 (binding solution)
A58151 (proteinase K)
A58152 (proteinase K dye)

  Application Note: Detection of SARS-CoV-2 in fecal samples and wastewater
 

Microplates and tip combs for KingFisher magnetic processing instruments

Thermo Scientific KingFisher plastics are ideal for magnetic particle processing due to their low binding affinity for biomolecules. Both the yield and the quality of the isolated RNA are significantly improved with special Thermo Scientific KingFisher plates and tips.

How to use MagMAX kits and Kingfisher instruments for SARS-CoV-2 viral RNA extraction

See how-to videos to learn how to use the MagMAX Viral/Pathogen Nucleic Acid Isolation kits with KingFisher Instruments and saliva collection devices.

How to use the MagMAX Viral/Pathogen Kit with the KingFisher Flex Purification System

Watch how to extract RNA for SARS-CoV-2 research and testing using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit with the KingFisher Flex Purification System for high-throughput nucleic acid isolation.

How to use the MagMAX Viral/Pathogen Kit with the KingFisher Duo Prime Purification System

Watch how to extract RNA for SARS-CoV-2 research and testing using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit with the KingFisher Duo Prime Purification System for medium-throughput nucleic acid isolation.

How to use the MagMAX Viral/Pathogen Kit using the manual extraction protocol for SARS-CoV-2 sample preparation

Watch how to extract RNA for SARS-CoV-2 research and testing using the MagMAX Viral/Pathogen kit using the manual extraction protocol for SARS-CoV-2 sample preparation.

How to use a Spectrum DNA SDNA-1000 saliva collection kit

Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit.

How to use a DNA Genotek OMNIgene ORAL (OM-505) collection kit

Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit.

How to use a 50 mL conical tube for saliva collection

Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit.

How to use a GeneFIX Saliva DNA/RNA collection device

Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit.

Kingfisher protocol files for SARS-CoV-2 testing

KingFisher Flex protocols for viral RNA extraction

The following protocols can be used for either the MagMAX Viral/Pathogen Kit (MVP I) and MagMAX Viral/Pathogen II Kit (MVP II) for SARS-CoV-2 testing, which supports sample input volume reduction of 400 μL to 200 μL, and reduction from 3 wash steps to 2 wash steps. Protocols can be downloaded for the KingFisher Flex 96 Deep-well Head (5400630).

Protocol typeStandard wash (3 wash steps)Reduced wash (2 wash steps)
200 μL scriptMVP_Flex_200ul.bdzMVP_2Wash_200_Flex.bdz
400 μL scriptMVP_Flex_400ul.bdzMVP_2Wash_400_Flex.bdz

 

KingFisher Flex protocol for extraction of viral RNA from saliva

The following protocol can be used for either the MagMAX Viral/Pathogen Kit (MVP I) and MagMAX Viral/Pathogen II Kit (MVPII) for SARS-CoV-2 testing, which supports the isolation of viral nucleic acid from 200 µL of saliva (raw or preserved).

Nucleic acidSample typeKit nameSKUFormatSoftware protocol
RNASalivaMagMAX Viral/Pathogen Kit (MVP I)
A48310
A42352
up to 2,000 preps
up to 200 preps
MagMAX Viral/Pathogen RNA Saliva
 Flex

 

Viral RNA extraction kits optimized for SARS-CoV-2

MagMAX magnetic bead-based kits offer flexible, highly efficient methods for purifying nucleic acids. The following products are specifically recommended for SARS-CoV-2 viral RNA extraction.

 MagMAX Microbiome Ultra Nucleic Acid Isolation Kits

  MagMAX Viral/Pathogen Nucleic Acid Isolation Kit
MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (MVP I)MagMAX Viral/Pathogen Nucleic Acid Isolation Kit MagMAX Bulk Reagents for Viral RNA extraction
  MagMAX Bulk Reagents for Viral RNA extraction product image
MagMAX Prime Viral/Pathogen NA Isolation kit  Viral/Pathogen NA Isolation Kit
Sample typesStool, swabs, transport media, culture media, urine, saliva, and soilUniversal viral transport media (VTM), bronchoalveolar lavage (BAL) and other respiratory research samples, urine, whole blood, and salivaSwabs in transport media (respiratory), saliva, urine
Best forSolid samples and wastewaterLarge sample volumesHighly customized protocolsRunning various sample types on a single plate
Cat. No.A42357 (100 preps), with bead plate
A42358 (100 preps), with bead plate
A48310 (up to 2,000 preps/kit)
A42352 (up to 200 preps/kit)
A42359 (binding solution)
A42360 (wash solution)
A42362 (binding beads)
A42363 (proteinase K)
A42364 (elution buffer)
A58145 (up to 600 preps/kit)
A58147 (binding beats)
A58148 (elution buffer)
A58149 (wash I solution)
A58150 (binding solution)
A58151 (proteinase K)
A58152 (proteinase K dye)

  Application Note: Detection of SARS-CoV-2 in fecal samples and wastewater
 

Microplates and tip combs for KingFisher magnetic processing instruments

Thermo Scientific KingFisher plastics are ideal for magnetic particle processing due to their low binding affinity for biomolecules. Both the yield and the quality of the isolated RNA are significantly improved with special Thermo Scientific KingFisher plates and tips.

How to use MagMAX kits and Kingfisher instruments for SARS-CoV-2 viral RNA extraction

See how-to videos to learn how to use the MagMAX Viral/Pathogen Nucleic Acid Isolation kits with KingFisher Instruments and saliva collection devices.

How to use the MagMAX Viral/Pathogen Kit with the KingFisher Flex Purification System

Watch how to extract RNA for SARS-CoV-2 research and testing using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit with the KingFisher Flex Purification System for high-throughput nucleic acid isolation.

How to use the MagMAX Viral/Pathogen Kit with the KingFisher Duo Prime Purification System

Watch how to extract RNA for SARS-CoV-2 research and testing using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit with the KingFisher Duo Prime Purification System for medium-throughput nucleic acid isolation.

How to use the MagMAX Viral/Pathogen Kit using the manual extraction protocol for SARS-CoV-2 sample preparation

Watch how to extract RNA for SARS-CoV-2 research and testing using the MagMAX Viral/Pathogen kit using the manual extraction protocol for SARS-CoV-2 sample preparation.

How to use a Spectrum DNA SDNA-1000 saliva collection kit

Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit.

How to use a DNA Genotek OMNIgene ORAL (OM-505) collection kit

Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit.

How to use a 50 mL conical tube for saliva collection

Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit.

How to use a GeneFIX Saliva DNA/RNA collection device

Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit.

Kingfisher protocol files for SARS-CoV-2 testing

KingFisher Flex protocols for viral RNA extraction

The following protocols can be used for either the MagMAX Viral/Pathogen Kit (MVP I) and MagMAX Viral/Pathogen II Kit (MVP II) for SARS-CoV-2 testing, which supports sample input volume reduction of 400 μL to 200 μL, and reduction from 3 wash steps to 2 wash steps. Protocols can be downloaded for the KingFisher Flex 96 Deep-well Head (5400630).

Protocol typeStandard wash (3 wash steps)Reduced wash (2 wash steps)
200 μL scriptMVP_Flex_200ul.bdzMVP_2Wash_200_Flex.bdz
400 μL scriptMVP_Flex_400ul.bdzMVP_2Wash_400_Flex.bdz

 

KingFisher Flex protocol for extraction of viral RNA from saliva

The following protocol can be used for either the MagMAX Viral/Pathogen Kit (MVP I) and MagMAX Viral/Pathogen II Kit (MVPII) for SARS-CoV-2 testing, which supports the isolation of viral nucleic acid from 200 µL of saliva (raw or preserved).

Nucleic acidSample typeKit nameSKUFormatSoftware protocol
RNASalivaMagMAX Viral/Pathogen Kit (MVP I)
A48310
A42352
up to 2,000 preps
up to 200 preps
MagMAX Viral/Pathogen RNA Saliva
 Flex

 


SARS-CoV-2 Research Solutions

From viral research and epidemiological studies to potential vaccine development.

Learn more ›

For Research Use Only. Not for use in diagnostic procedures.

For Research Use Only. Not for use in diagnostic procedures.