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The Dynabeads® kilobaseBINDER™ Kit is designed to immobilize long (>2 kb) double-stranded DNA molecules. Traditional immobilization of PCR products to Streptavidin-coupled Dynabeads® has shown that the amount of DNA immobilized is dependent on fragment size. The binding efficiency is significantly reduced, due to steric hindrance, when fragment size exceeds 2 kb (Figure 1). The kilobaseBINDER™ kit includes a patented immobilization activator in the Binding Solution, which significantly increases binding efficiency of long, biotinylated DNA molecules.
Figure 1. B&W buffer (red) is the standard Binding and Washing Buffer recommended for use with Streptavidin-coupled Dynabeads®. The specially designed Binding Solution (green) significantly increases the binding capacity.
Figure 2. DNA-coated Dynabeads® (red) assemble a mitotic spindle (green) in Xenopus egg extracts, indistinguishable from those in normal dividing cells (1). Binding capacity for 8kb biotinylated DNA fragments was routinely 20 µg (3.8 pmol) per mg Dynabeads®. Courtesy of R. Heald, E Karsenti and A. Hyman, EMBL, Heidelberg, Germany.
For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.