RNA Extraction for qPCR and RT-PCR

Reverse transcription-polymerase chain reaction (RT-PCR) is a process that transforms RNA to cDNA via a reverse transcriptase, then replicates it with a DNA polymerase. To get good results from reverse transcription, the starting RNA material must be of sufficient quantity, intact, and free of genomic DNA.

Real-time PCR—also known as quantitative PCR or qPCR—quantifies the copy number of PCR templates, such as DNA or complementary DNA (cDNA). Invitrogen kits and reagents for RNA extraction for qPCR are designed to yield the quality and quantity of RNA you need for RT-PCR reactions

Learn the steps involved in RNA isolation

RNA samples should be purified prior to qPCR. Genomic DNA contamination can lead to false positive RT-PCR results. Thermo Fisher Scientific offers a number of DNases that can be used to eliminate genomic DNA that may be present in your RNA preparation.

The DNA-free DNase Treatment and Removal Kit contains everything you need to remove contaminating DNA from RNA samples and remove the DNase without the use of Proteinase K treatment and organic extraction.

The TURBO DNase enzyme kit includes a hyperactive enzyme engineered from wild-type bovine DNase. The proficiency of TURBO DNase enzyme in binding very low concentrations of DNA means that the enzyme is particularly effective in removing trace quantities of DNA contamination.