RNA Phosphoramidites

Thermo Scientific phosphoramidites with ribonucleobases for RNA oligo synthesis are built to meet the varying requirements of oligonucleotide manufacturers from the proof of concept of research application (research use only, RUO) and development (R&D) to the process development of therapeutic and diagnostic applications. Backed by documentations and certificate of analysis, choose from multiple quality grades of standard, TheraPure, and TheraPure with fast deprotect phosphoramidites that best fit your needs.

Premium-grade and high-purity phosphoramidites are manufactured under extreme quality control and QC testing before product release. Ideal for any therapeutics application with excellent batch-to-batch reproducibility.

Extensive QC testing to help ensure impurities and residual solvents are within the stringent requirement for oligo therapeutics.

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Available TheraPure RNA phosphoramidites are compatible with the following oligonucleotide synthesizers: 

• Applied Biosystems / AKTA Synthesizer
• Expedite Synthesizer
• MerMade Synthesizer
• Bulk
  

Compatible products are in amber bottles with serum vial neck style as ready-to-ship catalog SKU.

Available catalog SKUs are supplied in 30 mL amber bottle with a 20-400 finish neck style, which is compatible with the Expedite synthesizer for oligo synthesis.

Catalog SKUs available in 30 mL amber bottle with a 28-400 finish neck style are compatible with the MerMade synthesizer for oligo synthesis.

Compatible catalog SKUs are offered in great value pack. Supplied in 250 mL opaque bottle with a wide mouth neck style, which is compatible with most bulk oligonucleotide synthesizers for oligo synthesis.

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Fast deprotect phosphoramidites permit the removal of base protecting groups under mild or accelerated conditions without the need for special reagents and are available in premium TheraPure grade. Excellent for applications requiring mild deprotection to preserve the integrity of oligonucleotides.

Rigorous QC testing is done to help ensure high purity with the removal of base-protecting groups allowed without needing special reagents. 

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* When using both of PAC-rA (Cat No. 27-1803) and iPrPAC-rG (Cat No. 27-1806), select Ac-rC (Cat No. 27-1805) and rU (Cat No. 27-1804) to minimize the time for post-synthesis deprotection.

** The acetyl-protected rG (Ac-rG, Cat No. SY3000) amidite is a suitable replacement for iBu-rG (Cat No. 2706) and is ideal for synthesis of longmers. Oligonucleotide deprotection times can be reduced as much as 25%. 

Available TheraPure RNA phosphoramidites with fast deprotect features are compatible with the following oligonucleotide synthesizers: 

• Applied Biosystems / AKTA Synthesizer
• Expedite Synthesizer

Compatible products are in amber bottles with serum vial neck style as ready-to-ship catalog SKU.

Ready-to-ship in 30 mL amber bottle with a 20-400 finish neck style for oligo synthesis.

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Traditional base protection methods to help ensure quality and reliability of routine oligonucleotide synthesis.

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Available and ready-to-ship in amber bottles with serum vial neck style for RNA oligo synthesis.

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HPLC-MS, GC, ³¹P NMR, and Karl Fischer oven titration are used to assess the purity of each phosphoramidite lot. The rigorously controlled manufacturing processes help minimize levels of reactive P(III) impurities and accumulation of by-products during oligo synthesis. Processes are monitored from material procurement through final QC and product release for batch-to-batch reproducibility under the ISO 9001:2015–certified quality management system.

Phosphoramidites are the key raw material and building blocks of oligonucleotide therapeutics. Incorporating the quality testing step into the manufacturing process development from the very beginning is a best practice needed for regulatory documentation. 

For the successful synthesis of a therapeutic from discovery to market, a thorough understanding on the analysis of phosphoramidites and impurity is crucial. Here is information on the analytical method used and the details on the instrument conditions used to test the quality of the raw material phosphoramidites.

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