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Polymerase Chain Reaction (PCR)
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Real-Time PCR
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Real-Time PCR Learning Center
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Real-Time PCR Basics
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Real-Time PCR Troubleshooting Tool
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Gene Expression | Quantitation Troubleshooting
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Abnormal Amplification Curves
Abnormal Amplification Curves
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‹
Gene Expression | Quantitation Troubleshooting
Abnormal Amplification Curves
Your amplification curves have a sigmoidal shape
Amplification occurs later than expected
Amplification of the No Template Control (NTC)
›
No Amplification
›
Poor PCR Efficiency
›
Normal Amplification Curve
›
‹
Gene Expression | Quantitation Troubleshooting
Abnormal Amplification Curves
Your amplification curves have a sigmoidal shape
Amplification occurs later than expected
Amplification of the No Template Control (NTC)
›
No Amplification
›
Poor PCR Efficiency
›
Normal Amplification Curve
›
Your amplification curves have a sigmoidal shape
Amplification occurs later than expected
For Research Use Only. Not for use in diagnostic procedures.