You may not have designated ROX™ dye as the reference dye

If you do not designate ROX™ dye as a reference:

• For the 7900 SDS software, the software will not autocall the alleles.
• For the 7500 FAST System SDS software, trailing clusters will appear in the allelic discrimination plots, as shown in the figure below.

Using ROX™ dye as the passive reference dye

7900 SDS Software

Note: The instructions below are for v.2.3 of the software. For other versions of the software, see the user guide.

1. Click the well of interest to select it.
2. Click the Setup tab.
3. At the bottom of the tab, select ROX in the Passive reference list.
4. Click green arrow  to reanalyze the plate.

7500 FAST System SDS Software

Note: The instructions below are for v.1.3 of the software. For other versions of the software, see the user guide.

1. Click the Setup tab.
2. In the plate document, double-click the well. The Well Inspector dialog box appears.
   
   

   

3. For Passive Reference, select ROX and then click Close.
4. Click  green arrow to analyze the plate.
   
   ROX™ dye is a passive reference dye that improves the precision of the results by compensating for small fluorescent fluctuations, such as bubbles and small well to well variations that occur in the plate.