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The Applied Biosystems TaqMan hPSC Scorecard Panel assesses pluripotency and trilineage differentiation potential using real-time qPCR assays and intuitive data analysis software. The hPSC Scorecard assay was developed in collaboration with Alex Meissner, and follows from his landmark publication (Cell 2011 144:439–452). The accompanying analysis software gives researchers anywhere in the world the ability to compare the gene expression profiles of their cell lines to a common reference set.
Hear from Jennifer Moore at RUCDR/Infinite Biologics on the benefits Scorecard has to offer.
See which instruments are compatible with the TaqMan hPSC Scorecard Panel and how to set them up.
The TaqMan hPSC Scorecard Panel includes access to free, intuitive web-based analysis software that allows researchers to quantitatively compare the gene expression profile of their sample to that of a reference set.
Lacking the resources or instruments to use the Scorecard Panel? Let us do it for you!
The TaqMan hPSC Scorecard Panels quantitatively confirms pluripotency (trilineage differentiation potential)
for both embryonic and induced pluripotent stem cell lines. The hPSC Scorecard Panel (available in 96 and 384-well format) contains 94 predefined TaqMan Gene Expression assays (including endogenous controls) dried-down in assay plate wells. The panel of genes was developed in collaboration with Alex Meissner, and based on his landmark publication (Cell 144:439-452 (2011)).
Undifferentiated ESC/iPSC
Differentiated ESC/iPSC: Embryoid bodies (week 1) - or - Directly differentiated cells.
Sample requirements: >1 x 106 cells/sample
TRIzol organic phase extraction
Sample requirements: 1 mL TRIzol reagent per 6 cm culture dish
[RNA] (mg/mL) = A260 * 40
Recommended A260/A280 ratio = 1.6 – 1.8
8 reverse transcription (RT) reactions per RNA sample
Experiment type: Standard Curve
Analyze data from the TaqMan hPSC Scorecard Panel using the intuitive analysis software
Sign in to the hPSC Scorecard Analysis Software
Undifferentiated ESC/iPSC
Differentiated ESC/iPSC: Embryoid bodies (week 1) - or - Directly differentiated cells.
Sample requirements: >1 x 106 cells/sample
TRIzol organic phase extraction
Sample requirements: 1 mL TRIzol reagent per 6 cm culture dish
[RNA] (mg/mL) = A260 * 40
Recommended A260/A280 ratio = 1.6 – 1.8
8 reverse transcription (RT) reactions per RNA sample
Experiment type: Standard Curve
Analyze data from the TaqMan hPSC Scorecard Panel using the intuitive analysis software
Sign in to the hPSC Scorecard Analysis Software
For Research Use Only. Not for use in diagnostic procedures.