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NucGreen Dead 488 ReadyProbes Reagent is a cell-impermeant stain that emits bright green fluorescence when bound to DNA. Since this reagent stains cells that lack plasma membrane integrity, it is extremely useful to measure viability and to measure cytotoxicity in kinetic live-cell assays. NucGreen Dead 488 reagent is also suitable for staining nuclei in fixed-cell preparations and tissue sections.
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NucGreen Dead 488 | |
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Excitation/Emission | 504/523 nm |
Standard filter set | FITC or GFP |
EVOS Light Cube | FITC |
Storage conditions | Room temperature |
HeLa cells stained with NucGreen Dead 488 ReadyProbes reagent, indicating loss of plasma membrane integrity.