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High-fidelity PCR enzymes are used for applications requiring high accuracy during DNA amplification such as cloning, sequencing or mutagenesis. Thermo Scientific Phusion high-fidelity DNA polymerases were created by fusing a dsDNA-binding domain to a Pyrococcus polymerase–like proofreading enzyme. Due to this unique protein fusion engineering, Phusion DNA Polymerases have very low error rates, fast extension rates, and high inhibitor tolerance.
Phusion high-fidelity DNA polymerases are available in a variety of formats. Please reference the table below to find an appropriate Phusion format for your application.
| NEWPhusion Plus DNA Polymerase | Phusion High-Fidelity DNA Polymerase | Phusion Hot Start II DNA Polymerase | Phusion Flash High-Fidelity PCR Master Mix | Phusion U Hot Start DNA Polymerase | Phusion U Multiplex PCR Master Mix | |
|---|---|---|---|---|---|---|
| Fidelity (vs. Taq enzyme) | >100x | 52x | 52x | 25x | 25x | 25x |
| Tm calculation required | No | Yes | Yes | Yes | Yes | Yes |
| Enhanced specificity (hot-start version) | Yes | No | Yes | Yes | Yes | Yes |
| Amplification length | Up to 20 kb | Up to 20 kb | Up to 20 kb | Up to 20 kb | Up to 20 kb | Up to 2.5 kb |
| Speed | 15–30 sec/kb | 15–30 sec/kb | 15–30 sec/kb | <15 sec/kb | 15–30 sec/kb | 15–30 sec/kb |
| GC-rich format | Yes (new GC enhanced) | Yes | Yes | No | Yes | No |
| dUTP incorporation | No | No | No | No | Yes | Yes |
| Designed for multiplex PCR | No | No | No | No | No | Yes, up to 20 targets |
| Stand-alone enzyme format* | ||||||
| 2x master mix format* |
* All stand-alone enzymes are supplied with their reaction buffers. The 2x master mix formats include all necessary PCR components except the template and primers.
** Contains green loading dyes and a density reagent for direct gel loading of PCR products
Do you need a DNA polymerase with higher fidelity and/or performance than Phusion enzymes? Discover Invitrogen Platinum SuperFi II DNA Polymerasewith >300x Taq fidelity and a simplified PCR protocol.
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