Nuclear Receptor Binding Assays

Our LanthaScreen Nuclear Receptor Competitive Binding Assay contains all the needed reagents you need to conduct compound affinity testing: 

• Purified nuclear receptor-LBD protein
• A proprietary fluorescent Fluormone™ ligand
  
• An optimized proprietary buffer system
• Terbium-labeled anti-GST antibody

The nuclear receptor (NR) protein is indirectly labeled with terbium via the anti-GST antibody bound to the GST tag on the receptor. When this labeled protein binds to the Fluormone™ ligand, a high TR-FRET ratio will result. If a test compound that binds to the NR protein is added to the reaction, the test compound will compete with the Fluormone™ ligand, resulting in a lower TR-FRET ratio. Since the change in TR-FRET ratio only occurs in the presence of a competitor, it can be used as a convenient and accurate indicator of the relative affinity of the test compound for the NR (Figure 1 below).

Figure 1. Assay schematic of a Nuclear Receptor LanthaScreen®