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Targeted genotyping by sequencing (GBS) is the latest innovation in genomics-assisted breeding programs. Targeted GBS uses a powerful multiplexed approach that enables users to interrogate hundreds to thousands of markers across hundreds to thousands of samples simultaneously. It includes NGS-based assays designed using customer-provided markers or catalog panels designed with publicly available markers.
Thermo Fisher Scientific offers two targeted genotyping by sequencing solutions:
The table below highlights the key features of our Applied Biosystems AgriSeq and targeted GBS technology.
AgriSeq targeted GBS solutions | |
---|---|
Requires Ion Torrent instrument | Yes |
SNPs per panel | 50–5,000 |
Ability to discover new variants | Yes |
Expected call rates* | –95% |
Processing time | 2 days |
Panel design | 2–6 weeks for custom panel design |
Sample throughput/week | Up to 7,680** |
Cost per sample | Low |
* Depends on level of genome annotation. ** Based on panel size with one instrument and one technician.