Recombinant Rabbit Polyclonal Antibodies

Recombinant rabbit polyclonal antibodies consist of a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. These antibodies offer a unique advantage by combining the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies. Additionally, they help provide the consistency that can only be achieved through recombinant antibody production. Similar to traditional polyclonal antibodies, recombinant polyclonal antibodies have the ability to recognize multiple epitope sites on the target molecule. This unique characteristic enables enhanced detection sensitivity, especially for targets with low abundance. However, what sets recombinant polyclonal antibodies apart is their known composition of heavy and light chains, which can be replicated consistently in every production lot. This eliminates the inherent biological variability that is typically associated with subsequent polyclonal lot reproductions. With recombinant polyclonal antibodies, researchers can enjoy the benefits of high sensitivity and reproducibility, helping provide a reliable and consistent tool for their experiments and assays.

See all recombinant rabbit polyclonal antibodies

With a wide range of antibodies available, we offer solutions for various applications, including western blotting, immunocytochemistry, flow cytometry, and more. Our antibodies undergo rigorous advanced verification processes to ensure optimal performance, specificity, and sensitivity. This comprehensive validation helps provide researchers with confidence in the reliability and accuracy of our antibodies. By choosing Invitrogen Recombinant antibodies, customers can access a diverse portfolio and trust in the quality and performance of our extensively verified products.

Recombinant rabbit polyclonal antibody production relies on molecular cloning of heavy and light chain encoding genes. The process starts with immunization of rabbits with the desired antigen, followed by isolation and propagation of peripheral blood mononuclear cells (PBMCs). The DNA from these cells is then cloned as a library and further screened using desired end-application to select an exceptional candidate for antibody production. The shortlisted DNA libraries are then scaled-up in vitro using mammalian cell culture followed by large-scale antibody production.

Recombinant rabbit polyclonal antibodies, generated using recombinant DNA technology, offer distinct advantages over conventional rabbit polyclonal antibodies. They provide precise control and modification of the antibody sequence, helping ensure accurate targeting. Moreover, recombinant antibodies exhibit excellent lot-to-lot reproducibility as they are derived from banked DNA, avoiding cell drift and maintaining consistent expression patterns. These antibodies also demonstrate enhanced specificity and sensitivity due to advanced screening processes, resulting in optimal binding capabilities. In contrast, conventional rabbit polyclonal antibodies may exhibit variability between production lots and have limited customization options. The unique features of recombinant rabbit polyclonal antibodies make them a valuable choice for researchers seeking reliable, consistent, and customizable antibody solutions.

Recombinant rabbit polyclonal antibodies provide exceptional specificity and sensitivity. These antibodies undergo a comprehensive screening process to evaluate their binding capabilities and those clones with the highest affinity for the target molecule are selected. This stringent selection process helps ensure that the recombinant rabbit polyclonal antibodies specifically recognize and bind to the intended target with precision.

For instance, let's consider the example of the SOD1 Recombinant Polyclonal Antibody (Figure 1). This antibody has been successfully employed to detect SOD1 protein across various cell lines, demonstrating its versatility and reliability in different experimental settings. Additionally, the antibody exhibits remarkable specificity, as evidenced by the results obtained from siRNA mediated knockdown analysis. Even under conditions where the expression of the target protein is reduced, this recombinant rabbit monoclonal antibody maintains high specificity, helping to ensure accurate and reliable detection.

Most recombinant rabbit polyclonal antibodies undergo an extensive functional validation process to ensure optimal performance across various applications, such as western blot, immunocytochemistry, flow cytometry, and immunohistochemistry. This thorough validation helps ensure that our antibodies consistently deliver reliable and accurate results.

For example, in Figure 2, the c-Jun Recombinant Polyclonal Antibody (2HCLC) has undergone comprehensive validation in multiple applications. It has been extensively tested using western blot, immunocytochemistry, and chromatin immunoprecipitation to assess its functionality and specificity. Additionally, this antibody has been further evaluated for specificity through CRISPR-Cas9 knockout experiments. Through our rigorous validation procedures, we help ensure that antibodies meet the highest standards of quality and performance, helping provide researchers with reliable tools for their experiments.

* The use or any variation of the word “validation” refers only to research use antibodies that were subject to functional testing to confirm that the antibody can be used with the research techniques indicated. It does not ensure that the product(s) was validated for clinical or diagnostic use.