Immunohistochemistry Information

Find protocols, educational webinars, scientific posters and more to help you design your immunohistochemistry experiments.

Immunohistochemistry features

BioProbes article

Bioprobes

Immune checkpoint antibodies for flow cytometry, IHC, and functional bioassays

This article features an overview of the current antibodies available to act as immune checkpoints for T cell defense against invading tumors.

Scientific poster

scientific-poster-immi

Incorporating histological analysis into existing high-content imaging

To address the need for quantitative colorimetric imaging, we have developed a method for analyzing histological samples on a high-content imaging instrument using a specialized brightfield unit


Immunohistochemistry learning resources

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TypeTitleCategories
BioProbes articles (Issues 50–present day)BioProbes Journal of Cell Biology Applicationcell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification
Pierce Protein MethodsOverview of immunohistochemistry (IHC)antibodies, fixation, immunohistochemistry (IHC)
Pierce Protein MethodsImmunohistochemistry methods articlesantibodies, fixation, immunohistochemistry (IHC)
ProtocolImmunohistochemical staining of formalin-fixed paraffin-embedded tissuesgeneral ab staining, imaging, immunohistochemistry (IHC)
ProtocolIHC frozen tissue—Indirect method (purified)general ab staining, imaging, immunohistochemistry (IHC)
ProtocolIHC frozen tissue—Indirect method (biotin)general ab staining, imaging, immunohistochemistry (IHC)
ProtocolIHC frozen tissue—Direct methodgeneral ab staining, imaging, immunohistochemistry (IHC)
ProtocolIHC FFPE Tissue Trypsin Digestion—Direct Methodgeneral ab staining, imaging, immunohistochemistry (IHC)
ProtocolIHC FFPE tissue trypsin digestion antigen retrieval—Indirect methodgeneral ab staining, imaging, immunohistochemistry (IHC)
ProtocolIHC FFPE tissue low pH antigen retrieval—Indirect methodgeneral ab staining, imaging, immunohistochemistry (IHC)
ProtocolIHC FFPE tissue low pH antigen retrieval—Direct methodgeneral ab staining, imaging, immunohistochemistry (IHC)
ProtocolIHC FFPE tissue high pH antigen retrieval—Indirect methodgeneral ab staining, imaging, immunohistochemistry (IHC)
ProtocolIHC FFPE tissue high pH antigen retrieval—Direct methodgeneral ab staining, imaging, immunohistochemistry (IHC)
Scientific poster (2015)Colorimetric dual labeled EdU/BrdU technology demonstrates contextual information and dynamics of proliferation in tissue apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC)
Scientific poster (2016)Correlating internalization and potency to accelerate antibody discovery and developmentbrightfield microscopy, colorimetric, endocytosis and phagocytosis, EVOS,fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC)
Scientific poster (2016)Incorporating histological analysis into existing high content imaging antibodies, ArrayScan, brightfield microscopy, fluorescence microscopy/fluorescence imaging, high content analysis, immunohistochemistry (IHC)
Scientific poster (2016)Click colorimetric EdU proliferation and TUNEL: Click chemistry for brightfield microscopyapoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC)
VideoImmunohistochemistry—Tips and tricks for weak or no stain Immunohistochemistry application, offers a spatial visualization and location of an antigen in tissue sections antibodies, brightfield microscopy, colorimetric, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), secondary detection
VideoImmunohistochemistry—Tips and tricks for high background Immunohistochemistry application offers a spatial visualization and location of an antigen in tissue sections. This video offers troubleshooting tips for issues falling into the "high background" category for IHC antibodies, brightfield microscopy, colorimetric, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), secondary detection

For Research Use Only. Not for use in diagnostic procedures.