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Fluorescence Imaging Information
Fluorescence imaging is a powerful tool for researchers but requires a significant amount of knowledge and skill to get accurate results. Here you will find a wide variety of tools to help you get the most out of your experiments.
Fluorescence imaging features
On-demand webinar
Fixed cell imaging—Five steps for publication-quality images
With over 40 years dedicated to cell imaging research, we offer long-proven tools and protocols to help confidently create quality cell images the first time. This on-demand webinar covers the 5 essential steps to getting great images.
eBook
Live cell imaging
Published by Wiley, this Essential Knowledge Briefing provides a short, practical guide of the background and basics of live-cell imaging. Content includes basic history of live-cell imaging, case studies of live-cell imaging applications, and common problems and solutions for live-cell imaging.
Fluorescence imaging learning resources
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| Type | Title | Categories |
|---|---|---|
| Application note (2012) | Monitoring neurite morphology and synapse formation in primary neurons for neurotoxicity assessments and drug screening | ArrayScan, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, neuroscience, viability |
| Application note (2013) | Optimizing the high content analysis of cell morphology using multi-dimensional imaging on the Thermo Scientific ArrayScan XTI HCA Reader: Stem cell colonies | Alexa Fluor, antibodies, ArrayScan, confocal microscopy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, immunofluorescence (IF), microplate reader, particles, stem cell research |
| Application note (2013) | Multiplexed mitosis and apoptosis analysis | antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, particles |
| Application note (2014) | High content cell cycle screening: Pairing FUCCI technology with Thermo Scientific HCS Studio 2.0 Software and FCS Express Image cytometry | ArrayScan, cancer, cell cycle, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader |
| Application note (2014) | Elucidating the mechanism of cell death through the use of high content analysis | apoptosis, autophagy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis |
| Application note (2015) | Using time-lapse imaging with the EVOS FL Auto Imaging System | EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, hypoxia, live-cell imaging, onstage incubator |
| Application note (2015) | Guide to setting up hypoxic conditions on the EVOS FL Auto Imaging System with Onstage Incubator | EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, onstage incubator, phagocytosis |
| Application note (2015) | Quantitation of proliferating cells with the EVOS FL Auto Imaging System | antibodies, antibody labeling, ArrayScan, cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, onstage incubator, phagocytosis |
| Application note (2015) | Phagocytosis visualized on the EVOS FL Auto Imaging System with Onstage Incubator | CellLight, EVOS, fluorescence microscopy/fluorescence imaging, live-cell imaging, ReadyProbes |
| Application note (2015) | Image tiling and stitching using the EVOS FL Auto Imaging System | cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, Imaging sample preparation, ReadyProbes |
| Application note (2015) | Collecting Z-stack image sequences with the EVOS FL Auto Imaging System | cell structure-all, CellLight, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, ReadyProbes |
| Application note (2015) | Cell division and migration during wound healing visualized on the EVOS FL Auto Imaging System | cell health, cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging |
| Application note (2017) | Simple and accurate monitoring of adipogenesis | adipocyte, adipogenesis, automated cell counter, cell differentiation, Countess, EVOS, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, onstage incubator, stem cell research |
| Application note (2017) | Flow cytometry analysis of transcription factor expression during differentiation of hPSC-derived cardiomyocytes | antibodies, Attune/Attune NxT, cardiomyocytes, cell differentiation, flow cytometer/flow cytometry, fluorescence, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunophenotyping, stem cell research |
| BioProbes articles (Issues 50–present day) | BioProbes Journal of Cell Biology Application | cell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification |
| Book | Live cell imaging Published by Wiley, this Essential Knowledge Briefing provides a short, practical guide of the background and basics of live cell imaging. Content includes basic history of live cell imaging and case studies of live cell imaging applications Common problems and solutions with live cell imaging. | fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, onstage incubator |
| eBook | Live cell imaging Published by Wiley, this Essential Knowledge Briefing provides a short, practical guide of the background and basics of live cell imaging. Content includes basic history of live cell imaging and case studies of live cell imaging applications Common problems and solutions with live cell imaging. | fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, onstage incubator |
| Molecular Probes Handbook | TSA and other peroxidase-based signal amplification techniques—Section 6.2 | Alexa Fluor, antibodies, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), reagents, secondary detection, signal amplification |
| Molecular Probes Handbook | Microspheres—Section 6.5 | flow cytometer calibration, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, microscope calibration, particles |
| Molecular Probes Handbook | Introduction to signal amplification—Section 6.1 | antibodies, fluorescence microscopy/fluorescence imaging, secondary detection, signal amplification, western detection |
| Molecular Probes Handbook | Fluorescence microscopy accessories and reference standards—Section 23.1 | fluorescence microscopy/fluorescence imaging |
| Molecular Probes Handbook | Using the Fluorescence Spectraviewer—Note 23.1 | flow cytometer/flow cytometry, fluorescence, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins |
| Molecular Probes Handbook | Selecting optical filters for fluorescence microscopy—Note 23.2 | fluorescence microscopy/fluorescence imaging |
| Molecular Probes Handbook | Mitochondria in diseases—Note 12.2 | antibodies, cell structure-mitochondria, fluorescence microscopy/fluorescence imaging |
| Molecular Probes Handbook | Fluorescent probes for two-photon microscopy—Note 1.5 | fluorescence microscopy/fluorescence imaging, two-photon microscopy |
| Molecular Probes Handbook | Fluorescence resonance energy transfer (FRET)—note 1.2 | fluorescence microscopy/fluorescence imaging |
| Molecular Probes Handbook | Fluorescence polarization (FP)—note 1.4 | fluorescence microscopy/fluorescence imaging |
| Molecular Probes Handbook | Fluorescence correlation spectroscopy (FCS)—Note 1.3 | fluorescence microscopy/fluorescence imaging |
| Molecular Probes Handbook | Probes for the endoplasmic reticulum and golgi apparatus—Section 12.4 | antibodies, cell structure-er, cell structure-golgi, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Molecular Probes Handbook | Probes for mitochondria—Section 12.2 | antibodies, cell structure-mitochondria, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Molecular Probes Handbook | Probes for lysosomes, peroxisomes and yeast vacuoles—Section 12.3 | antibodies, cell structure-lysosomes, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Molecular Probes Handbook | Probes for the nucleus—Section 12.5 | antibodies, cell structure-nucleus, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, membrane permeability |
| Molecular Probes Handbook | A diverse selection of organelle probes—Section 12.1 | cell structure-er, cell structure-golgi, cell structure-lysosomes, cell structure-mitochondria, cell structure-nucleus, cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging |
| Molecular Probes Handbook | Slow-response probes—Section 22.3 | fluorescence microscopy/fluorescence imaging, live-cell imaging, membrane potential dyes |
| Molecular Probes Handbook | Introduction to potentiometric probes—Section 22.1 | cell structure-plasma membrane, fast-response probes, fluorescence microscopy/fluorescence imaging, fluorometer, ion channels, live-cell imaging, membrane potential, slow-response probes |
| Molecular Probes Handbook | Fast-response probes—Section 22.2 | cell structure-plasma membrane, fast-response probes, fluorescence microscopy/fluorescence imaging, fluorometer, ion channels, live-cell imaging, membrane potential |
| Molecular Probes Handbook | Other nonpolar and amphiphilic probes—Section 13.5 | carbocyanine dyes, cell structure-plasma membrane, DiI, fluorescence microscopy/fluorescence imaging, fluorescent lipid dyes, fluorometer |
| Molecular Probes Handbook | Introduction to membrane probes—Section 13.1 | cell structure-plasma membrane, fast-response probes, fluorescence microscopy/fluorescence imaging, fluorometer, ion channels, live-cell imaging, membrane potential, slow-response probes |
| Molecular Probes Handbook | Fatty acid analogs and phospholipids—Section 13.2 | cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging, fluorescent fatty acid analogs, fluorescent lipid dyes, fluorometer |
| Molecular Probes Handbook | Dialkylcarbocyanine and dialkylaminostyryl probes—Section 13.4 | carbocyanine dyes, cell structure-plasma membrane, DiI, fluorescence microscopy/fluorescence imaging, fluorescent lipid dyes, fluorometer |
| Molecular Probes Handbook | Probes for neurotransmitter receptors—Section 16.2 | brain hormones, cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging, fluorescent dyes, receptor binding |
| Molecular Probes Handbook | Probes for following receptor binding and phagocytosis—Section 16.1 | cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging, fluorescent dyes, internalization, membrane trafficking, phagocytosis, receptor binding, vesicle |
| Molecular Probes Handbook | Probes for tubulin and other cytoskeletal proteins—Section 11.2 | cell structure-tubulin, cytoskeleton, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins |
| Molecular Probes Handbook | Probes for actin—Section 11.1 | cell structure-actin, cytoskeleton, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins |
| Molecular Probes Handbook | Probes useful at near-neutral pH—Section 20.2 | BCECF, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, pH detection, pH indicators, SNARF |
| Molecular Probes Handbook | Probes useful at acidic pH—Section 20.3 | flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo |
| Molecular Probes Handbook | pH indicator conjugates—Section 20.4 | flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo |
| Molecular Probes Handbook | Overview of pH indicators—Section 20.1 | flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo |
| Molecular Probes Handbook | Nucleic acid stains—Section 8.1 | cell cycle, cell structure-nucleus, cell structure-plasma membrane, cell viability, DAPI, DNA binding dyes, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, membrane permeability, RNA binding dyes, SYTO, SYTOX |
| Molecular Probes Handbook | Tracers for membrane labeling—Section 14.4 | cell structure-plasma membrane, cell tracking, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging |
| Molecular Probes Handbook | Viability and cytotoxicity assay reagents—Section 15.2 | cell health, cell viability and function, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader |
| Molecular Probes Handbook | Viability and cytotoxicity assay kits for diverse cell types—Section 15.3 | cell health, cell viability and function, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader |
| Molecular Probes Handbook | Assays for apoptosis and autophagy—Section 15.5 | apoptosis, autophagy, fluorescent dyes, fluorescence microscopy/fluorescence imaging, flow cytometer/flow cytometry |
| Molecular Probes Handbook | Zenon Technology: Versatile reagents for immunolabeling—Section 7.3 | antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, secondary detection, Zenon |
| Protocol | TO-PRO-3 Stain | cell structure, imaging |
| Protocol | SYTOX Green Nucleic Acid Stain | cell structure, imaging |
| Protocol | SYTO 82 Nuclear Stain | cell structure, imaging |
| Protocol | SYTO 59 Nuclear Stain | cell structure, imaging |
| Protocol | ReadyProbes Cell Viability Imaging Kit, Blue/Red | cell viability, imaging |
| Protocol | ReadyProbes Cell Viability Imaging Kit, Blue/Green | cell viability, imaging |
| Protocol | Pharmacological induction of apoptosis with camptothecin | cell vitality, flow cytometry, high content analysis, imaging, microplate |
| Protocol | NucRed Live 647 ReadyProbes Reagent | cell structure, imaging |
| Protocol | NucRed Dead 647 ReadyProbes Reagent for viability | cell viability, imaging |
| Protocol | NucRed Dead 647 ReadyProbes Reagent for fixed cells | cell structure, imaging |
| Protocol | NucGreen Dead 488 ReadyProbes Reagent for viability | cell viability, imaging |
| Protocol | NucGreen Dead 488 ReadyProbes Reagent for fixed cells | cell structure, imaging |
| Protocol | NucBlue Live ReadyProbes Reagent | cell structure, imaging |
| Protocol | NucBlue Fixed Cell ReadyProbes Reagent | cell structure, imaging |
| Protocol | LIVE/DEAD Yeast Viability Kit | cell viability, imaging |
| Protocol | LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells | cell viability, imaging |
| Protocol | LIVE/DEAD Sperm Viability Kit for imaging | cell viability, imaging |
| Protocol | LIVE/DEAD Cell Imaging Kit (488/570) | cell viability, imaging |
| Protocol | LIVE/DEAD BacLight Bacterial Viability Kit | cell viability, imaging |
| Protocol | LIVE BacLight Bacterial Gram Stain Kit | cell viability, imaging |
| Protocol | Immunofluorescent staining of intracellular antigens on cultured cells | fluorescence microscopy/fluorescence imaging, general ab staining, imaging, immunofluorescence (IF) |
| Protocol | Image-iT DEAD Green Kit | cell viability, imaging |
| Protocol | Immunohistochemical staining of formalin-fixed paraffin-embedded tissues | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC frozen tissue—Indirect method (purified) | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC frozen tissue—Indirect method (biotin) | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC frozen tissue—Direct method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC FFPE Tissue Trypsin Digestion—Direct Method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC FFPE tissue trypsin digestion antigen retrieval—Indirect method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC FFPE tissue low pH antigen retrieval—Indirect method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC FFPE tissue low pH antigen retrieval—Direct method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC FFPE tissue high pH antigen retrieval—Indirect method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC FFPE tissue high pH antigen retrieval—Direct method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | ICC unfixed cells—Direct method | general ab staining, imaging |
| Protocol | ICC methanol fixed cells—Indirect method | general ab staining, imaging |
| Protocol | ICC methanol fixed cells—Direct method | general ab staining, imaging |
| Protocol | ICC formaldehyde fixed, permeabilized cells—Indirect method | general ab staining, imaging |
| Protocol | ICC formaldehyde fixed, permeabilized cells—Direct method | general ab staining, imaging |
| Protocol | ICC formaldehyde fixed cells—Indirect method | general ab staining, imaging |
| Protocol | ICC formaldehyde fixed cells—Direct method | general ab staining, imaging |
| Protocol | Hoechst 33342 for imaging | cell structure, imaging |
| Protocol | DAPI for fluorescence imaging | cell structure, imaging |
| Protocol | Colorimetric FFPE—trypsin digestion | colorimetric staining, general ab staining, imaging |
| Protocol | Colorimetric FFPE—Low pH antigen retrieval | colorimetric staining, general ab staining, imaging |
| Protocol | Colorimetric FFPE—High pH antigen retrieval | colorimetric staining, general ab staining, imaging |
| Protocol | Click-iT Plus EdU imaging kits | cell proliferation, imaging |
| Protocol | Click-iT EdU imaging kits | cell proliferation, imaging |
| Protocol | Click-iT EdU HCS assays | cell proliferation, imaging |
| Protocol | BrdU labeling and detection | cell proliferation, imaging |
| Protocol | ActinRed 555 ReadyProbes Reagent | cell structure, imaging |
| Protocol | ActinGreen 488 ReadyProbes Reagent | cell structure, imaging |
| Scientific poster (2006) | LipidTOX dyes for adipocyte staining in routine imaging applications | cell differentiation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, lipid detection, lipids, stem cell research, viability |
| Scientific poster (2006) | Cell cycle analysis using microplate cytometry: A comparison of laser and dye combinations | Acumen Explorer, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader |
| Scientific poster (2006) | New fluorescent reagents for the imaging-based assays for the analysis of drug-induced perturbations of cellular lipid metabolism | ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, lipid detection, lipids, microplate reader, viability |
| Scientific poster (2006) | Utility of 405 nm-excitable dyes in high content screening using an Acumen Explorer Microplate Cytometer | Acumen Explorer, apoptosis, cell cycle, cell health, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, violet laser-excited reagents |
| Scientific poster (2007) | Evaluation of click chemistry-based alternative to BrdU antibody labeling in tissue and cultured cells using fluorescence microscopy and flow cytometry | antibodies, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, microplate reader, nucleic acid labeling |
| Scientific poster (2007) | A new approach for the detection of intracellular glutathione by fluorescence microscopy and flow cytometry | ArrayScan, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, live-cell imaging, microplate reader, viability, violet laser-excited reagents |
| Scientific poster (2007) | Click chemistry-based detection of S-phase adherent cells using automated microscopy and image analysis | Alexa Fluor, antibodies, ArrayScan, cell cycle, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader |
| Scientific poster (2007) | Click catalyzed nucleic acid labeling as a novel replacement for BrdU antibody-based cell proliferation assay | Alexa Fluor, antibodies, antibody labeling, cell cycle, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling |
| Scientific poster (2007) | BacMam gene delivery of targeted fluorescent proteins, Organelle Lights for HCS | BacMam technology, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, live-cell imaging, nanocrystals, Qdot |
| Scientific poster (2008) | Living molecular probes for the next generation | BacMam technology, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, ion flux/ion channels, ion indicators, live-cell imaging |
| Scientific poster (2008) | Quantum dot (Qdot) labeling of gene expression in fresh frozen brain tissue using high-throughput in situ hybridization | in situ hybridization, brightfield microscopy, confocal microscopy,fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, high content analysis, nanocrystals, neuroscience, Qdot, tissue |
| Scientific poster (2008) | Characterization of DNA content, cyclin B1 and phosphorylated histone H3 with direct S-phase using EDU incorporation in multiparameter testing of cell lines with cell cycle blocking agents | Alexa Fluor, antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis |
| Scientific poster (2008) | Novel Click-IT TUNEL Assay for detection of cell death | Alexa Fluor, antibodies, apoptosis, Click-iT, cytotoxicity, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, immunocytochemistry (ICC), viability |
| Scientific poster (2008) | Effects of the thymidine analogues EdU and BrdU on cell viability and cycle progression | Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, viability |
| Scientific poster (2008) | ThiolTracker Violet dye, a new 405 nm excitable fluorescent probe for detecting intracellular thiols in imaging-based assays | ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, glutathione, high content analysis, microplate reader, protein labeling, violet laser-excited reagents |
| Scientific poster (2008) | Modified tumor cell protein expression and modification under physiological oxygen | Alexa Fluor, cancer, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, physiological oxygen level, protein labeling |
| Scientific poster (2008) | A new 405 nm excitable fluorescent probe of intracellular thiols for imaging and flow cytometric assays | ArrayScan, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, high content analysis, microplate reader, thiol modification, violet laser-excited reagents |
| Scientific poster (2009) | Cell-based assays for predictive hepatotoxicity measurements using high content imaging | Alexa Fluor, ArrayScan, cell structure-plasma membrane, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, high content analysis, microplate reader, nucleic acid quantitation, viability |
| Scientific poster (2009) | A non-radioactive method for detecting nascent protein synthesis by high-content imaging utilizing click chemistry | Alexa Fluor, apoptosis, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, protein detection |
| Scientific poster (2009) | Monitoring mitotic cells and DNA content by automated imaging and analysis | Alexa Fluor, cell cycle, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling, nucleic acid quantitation, viability |
| Scientific poster (2009) | Dual pulse labeling using a new thymidine analog 5-ethynyl-2'-deoxyuridine (EdU) to detect alterations in S-phase progression by fluorescence microscopy and flow cytometry | Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Scientific poster (2009) | High content imaging of endocytosis & phagocytosis using pHrodo conjugates | Alexa Fluor, ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, pH detection, phagocytosis, viability |
| Scientific poster (2009) | Click chemistry-based detection of nascent RNA synthesis using high content imaging and fluorescence microscopy | Alexa Fluor, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling, viability |
| Scientific poster (2009) | High content analysis of cytotoxicity by laser scanning fluorescence microplate cytometry | Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, viability |
| Scientific poster (2009) | Non-radioactive targeting of multiple classes of protein post-translational modifications (PTMs) with click chemistry | cell signaling, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling, western blotting |
| Scientific poster (2009) | High content imaging and analysis of mitotoxicity and cytotoxicity in fixed cells | cell structure-mitochondria, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, viability |
| Scientific poster (2009) | Quantitative analysis of genotoxicity and cytotoxicity to DNA damaging agents using high-content imaging | Alexa Fluor, antibodies, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling, nucleic acid quantitation, viability |
| Scientific poster (2009) | Metabolic labeling and click chemistry detection of stem cell markers | Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein labeling, stem cell research |
| Scientific poster (2009) | Click chemistry-based assays for fluorescence microscopy and high content imaging | Alexa Fluor, apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid detection, protein detection, viability |
| Scientific poster (2010) | Simultaneous analysis of cell death mechanisms and oxidative stress using Molecular Probes next generation reagents for imaging and flow cytometry | apoptosis, autophagy, caspase substrates, flow cytometry/flow cytometer, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, oxidative stress |
| Scientific poster (2010) | High content imaging of autophagy | antibodies, ArrayScan, autophagy, BacMam technology, cell signaling, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader |
| Scientific poster (2010) | Oxidative stress-induced protein modification: Application of clickable linoleic acid analogs | Alexa Fluor, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, microplate reader, oxidative stress, protein detection, protein enrichment |
| Scientific poster (2010) | Antibody- and fluorescent protein-based approaches to measuring autophagy in mammalian cells by fluorescence microscopy | Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging |
| Scientific poster (2010) | Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imaging | Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents |
| Scientific poster (2010) | Novel tools to enable high resolution gene expression by investigating the nascent transcriptome | Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, high content analysis, magnetic beads, nucleic acid labeling, PCR/qPCR, viability |
| Scientific poster (2010) | Click chemistry-based enrichment and identification of nascent and post-translationally modified proteins | cells, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling |
| Scientific poster (2010) | Autophagosomal accumulation perturbs Golgi structure without affecting other organelles: Implications for autophagosome biogenesis | Alexa Fluor, antibodies, autophagy, BacMam technology, cell structure-golgi, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging |
| Scientific poster (2010) | A new generation of cellular imaging probes for neurosciences research | autophagy, BacMam technology, CellLight, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels, ion indicators |
| Scientific poster (2010) | ABfinity antibodies: A novel approach to antibody development | Alexa Fluor, antibodies, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, immunocytochemistry (ICC), western blotting |
| Scientific poster (2010) | NA-Fluor Influenza Neuraminidase Assay Kit: Standardization of the classic MUNANA-based neuraminidase activity assay for monitoring antiviral resistance in influenza | drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, influenza, microbiology, microplate reader |
| Scientific poster (2010) | Simultaneous analysis of cell death mechanisms and oxidative stress using live cell fluorescence microscopy | apoptosis, ArrayScan, BacMam technology, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader, oxidative stress |
| Scientific poster (2010) | New fluorescent probes for live-cell imaging | autophagy, cell cycle, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, pH detection, phagocytosis |
| Scientific poster (2010) | The NA-XTD Influenza Neuraminidase Assay Kit: Extended-glow chemiluminescence assay system for highly sensitive influenza neuraminidase assays | chemiluminescence, drug discovery,fluorescence microscopy/fluorescence imaging, high content analysis, influenza, microbiology, microplate reader |
| Scientific poster (2010) | Non-cytotoxic near-IR DNA stain for cell cycle analysis in living cells | cell cycle, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, live-cell imaging, nucleic acid labeling, viability |
| Scientific poster (2010) | Click chemistry enrichment of cell surface glycoproteins | cells, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling |
| Scientific poster (2010) | Identification and characterization of O-GlcNAc modification of galectin-1 in mesenchymal stem cells using click chemistry | Alexa Fluor, BacMam technology, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, immunocytochemistry (ICC), protein enrichment, protein labeling, western blotting |
| Scientific poster (2010) | BacMam gene expression for functional and imaging applications | BacMam technology, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels |
| Scientific poster (2010) | Click chemistry-based universal enrichment columns for global identification of azide-modified proteins | Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling |
| Scientific poster (2010) | Dual pulse labeling using EdU and BrdU to monitor alterations in cellular proliferation by fluorescence microscopy and flow cytometry | Alexa Fluor, cell cycle, cell proliferation, Click-iT, drug discovery, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Scientific poster (2010) | A universal click chemistry-based enrichment column for the identification of multiple subclasses of PTM-modified proteins and newly synthesized proteins | Alexa Fluor, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling |
| Scientific poster (2011) | Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health | ArrayScan, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress, viability |
| Scientific poster (2011) | Acoustic cytometric identification and initial characterization of limbal stem cells isolated from human cornea | Attune/Attune NxT, cell culture/expansion, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, PCR/qPCR, stem cell research, tissue, violet laser-excited reagents |
| Scientific poster (2011) | Simultaneous analysis of cell death mechanisms and oxidative stress using high content imaging | Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress |
| Scientific poster (2011) | Primary human cells from Life Technologies: Offerings, scale and applications in HCS and HTS formats | Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, cell signaling, Click-iT, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis |
| Scientific poster (2011) | Validation of high content imaging and analysis assays for cell health and cytotoxicity | antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, cell health, cell proliferation, CellLight, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, pH detection |
| Scientific poster (2011) | The next step in the analysis of lymphocyte proliferation | Alexa Fluor, antibodies, Attune/Attune NxT, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, live-cell imaging, violet laser-excited reagents |
| Scientific poster (2011) | The next generation of cell-based imaging assays for apoptosis, autophagy and oxidative stress from Molecular Probes | Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress |
| Scientific poster (2011) | NA-Fluor and NA-XTD Influenza Neuraminidase Assays for neuraminidase quantitation and inhibition assays | chemiluminescence, drug discovery,fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, influenza, microbiology, microplate reader |
| Scientific poster (2012) | Cell-based analysis of oxidative stress, lipid peroxidation and lipid peroxidation-derived protein modifications using fluorescence microscopy | flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis, lipid peroxidation, microplate reader, oxidative stress, viability |
| Scientific poster (2013) | Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health | apoptosis, ArrayScan, caspase substrates, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress |
| Scientific poster (2013) | Cells coming to life: Tools for visualizing immune response | Attune/Attune NxT, endocytosis/phagocytosis, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunophenotyping, live-cell imaging, pH detection |
| Scientific poster (2013) | Illuminating endocytosis with targeted pH-sensitive fluorescent compounds | Alexa Fluor, ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, pH detection, phagocytosis, viability |
| Scientific poster (2014) | New fluorescent probes and sensors for visualizing endocytosis, lysosomal dynamics and autophagy | autophagy, cell structure-lysosomes, endocytosis/phagocytosis, autophagy, fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader |
| Scientific poster (2015) | Colorimetric dual labeled EdU/BrdU technology demonstrates contextual information and dynamics of proliferation in tissue | apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC) |
| Scientific poster (2015) | Visualizing the life and death of cells: novel probes and platforms | apoptosis, ArrayScan, autophagy, cell death, CellInsight, fluorescence microscopy/fluorescence imaging, high content analysis |
| Scientific poster (2015) | Intracellular detection of hypoxia in live cells | EVOS, fluorescence microscopy/fluorescence imaging, hypoxia, EVOS, onstage incubator |
| Scientific poster (2016) | Correlating internalization and potency to accelerate antibody discovery and development | brightfield microscopy, colorimetric, endocytosis and phagocytosis, EVOS,fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC) |
| Scientific poster (2016) | A novel non-cytotoxic fluorescent dye for cell proliferation analysis in flow cytometry | Attune/Attune NxT, cell health, cell proliferation, CellTrace, EVOS, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging |
| Scientific poster (2016) | Incorporating histological analysis into existing high content imaging | antibodies, ArrayScan, brightfield microscopy, fluorescence microscopy/fluorescence imaging, high content analysis, immunohistochemistry (IHC) |
| Scientific poster (2016) | Click colorimetric EdU proliferation and TUNEL: Click chemistry for brightfield microscopy | apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC) |
| Scientific poster (2017) | Matching refractive index of mounting media improves axial resolution and image quality in 3D biological samples | antifades, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, fluorescent proteins, imaging sample preparation, mountant, onstage incubator, photobleaching |
| Scientific poster (2017) | Hypoxia measurements in live and fixed cells using fluorescence microscopy and high-content imaging | EVOS, fluorescence microscopy/fluorescence imaging, high content analysis, hypoxia, live-cell imaging, onstage incubator |
| Tutorial | 1.2 Fixation–Fixed cell imaging: 5 steps for publication-quality images | fixation, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF) |
| Tutorial | 4.1 Mounting media–Fixed cell imaging: 5 steps for publication-quality images | antifade mounting media, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), mounting media |
| Tutorial | 3.2 Secondary antibody optimization–Fixed cell imaging: 5 steps for publication-quality images | antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), secondary antibodies, secondary antibody protocol, secondary detection |
| Tutorial | 4.2 Photobleaching and antifades–Fixed cell imaging: 5 steps for publication-quality images | antifades, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), photobleaching, Prolong Gold, Vectashield |
| Tutorial | 5.5 Ethical considerations–Fixed cell imaging: 5 steps for publication-quality images | ethical considerations, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, image analysis, immunofluorescence (IF), sample manipulation |
| Tutorial | 5.2 Imaging platforms-software–Fixed cell imaging: 5 steps for publication-quality images | FiFI software, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, ImageJ, imaging software, imaging systems, immunofluorescence (IF) |
| Tutorial | 1.1 Culture conditions–Fixed cell imaging: 5 steps for publication-quality images | EVOS, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, imaging hardware, imaging systems, immunofluorescence (IF), microscope |
| Tutorial | 1.5 Autofluorescence–Fixed cell imaging: 5 steps for publication-quality images | autofluorescence, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), tissue autofluorescence |
| Tutorial | 5.4 Image analysis with Celleste software–Fixed cell imaging: 5 steps for publication-quality images | Celleste software, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, image analysis, immunofluorescence (IF) |
| Tutorial | 1.4 Blocking–Fixed cell imaging: 5 steps for publication-quality images | dye charge blocking, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), nonspecific binding, protein blocking |
| Tutorial | 2.2 Primary antibody protocol optimization–Fixed cell imaging: 5 steps for publication-quality images | antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), primary antibodies, primary antibody protocol |
| Tutorial | 5.1 Imaging platforms-hardware–Fixed cell imaging: 5 steps for publication-quality images | EVOS, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, imaging hardware, imaging systems, immunofluorescence (IF), microscope |
| Tutorial | 3.5 Dye choice and special concerns–Fixed cell imaging: 5 steps for publication-quality images | dye choice, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, Fluorescence SpectraViewer, immunofluorescence (IF), label choice, multiparametric dye selection |
| Tutorial | 1.3 Permeabilization–Fixed cell imaging: 5 steps for publication-quality images | cell permeabilization, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF) |
| Tutorial | 3.4 Controls–Fixed cell imaging: 5 steps for publication-quality images | fixed-cell imaging, fluorescence microscopy/fluorescence imaging, imaging controls, immunofluorescence (IF), sample controls |
| Tutorial | 5.3 Image capture with EVOS FL Auto 2.0–Fixed cell imaging: 5 steps for publication-quality images | EVOS FL Auto 2.0, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, image capture, immunofluorescence (IF) |
| Tutorial | 3.3 Amplification techniques–Fixed cell imaging: 5 steps for publication-quality images | biotin and streptavidin, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), signal amplification, tyramide detection |
| Tutorial | 2.1 Primary antibody choice–Fixed cell imaging: 5 steps for publication-quality images | antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), primary antibodies |
| Tutorial | 3.1 Secondary antibody choice–Fixed cell imaging: 5 steps for publication-quality images | antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), secondary antibodies, secondary antibody, secondary detection |
| Tutorial | Labeling a purified antibody:Educational video on how to label your next antibody for imaging or fluorescence | antibodies, antibody labeling, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF) |
| Video | Setting up an automated scan routine on the EVOS FL Auto 2 Microscope | EVOS, fluorescence microscopy/fluorescence imaging, instrument set-up |
| Video | CellEvent Caspase 3/7 reagent and apoptosis detection HeLa cells were loaded with 50 nM TMRM (red) followed by 5 μM CellEvent Caspase 3/7 Substrate (green). Cells were then treated with 0.5 μM staurosporine to induce apoptosis. TMRM detects the mitochondrial membrane potential and the signal disappears as the apoptosis onsets. CellEvent detects apoptosis, with green signal. | apoptosis, cell health, CellEvent, fluorescence microscopy/fluorescence imaging, live-cell imaging |
| Video | Viability determination of HeLa cells using ReadyProbes Cell Viability Imaging Kit (Blue/Green) HeLa cells were loaded with NucBlue Live and NucGreen Dead (using 2 drops per ml) in complete media for 15 minutes at 37C. Staurosporine was then added to a final concentration of 1 µM and images were acquired every 30 min. for 18 hours using EVOS Auto Imaging System. All cells are stained with NucBlue Live, shown with blue nuclei. Over time an increase in the number of dead cells is observed as indicated by the appearance of green nuclei (NucGreen Dead). | cell structure-nucleus, cell viability, fluorescence microscopy/fluorescence imaging, live-cell imaging |
| Video | Adding oil to objectives on the EVOS FL Auto 2 Microscope See how easy it is to add oil to your objective without having to remove your sample when imaging with the EVOS FL Auto 2 Microscope. | EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up |
| Video | Reviewing Cell Images on the EVOS FL Auto 2 Microscope Cell images can be saved from the EVOS FL Auto 2 as a single field, scan, time lapse, and z-stack. Easily review your images and metadata or make adjustments to image brightness, contrast, or gamma and resave your images with the new settings. | EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up |
| Video | Using the locations tool on the EVOS FL Auto 2 Microscope Learn how to create custom locations to use in automated routines for scanning and time lapse experiments on the EVOS FL Auto 2 Cell Imaging System. | EVOS, fluorescence microscopy/fluorescence imaging, instrument set-up |
| Video | How to setup vessel maps on the EVOS FL Auto 2 Microscope The EVOS FL Auto 2 can accommodate most vessel types and sizes, including slides, multi-well plates, culture flasks, and petri dishes. See how easy it is to setup the EVOS FL Auto 2 for imaging with the vessel maps tool. | EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up |
| Video | How to add a scale bar and grid to cell images on the EVOS FL Auto 2 Microscope See how easy it is to add a scale bar and grid to your live or captured cell images on the EVOS FL Auto Microscope. You can adjust the grid size, color, and alignment to fit the needs of your experiment. | EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up |
| Video | Create z-stacks using the EVOS FL Auto 2 Microscope Capturing images at different Z-planes can be a powerful tool in fluorescence microscopy. This capability can reveal conditions not seen with standard widefield imaging and easier than ever to use with the EVOS FL Auto 2 microscope. | EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up |
| Video | LIVE DEAD Cell Imaging kit on the EVOS Auto Imaging System Time course of cell death visualized using the LIVE/DEAD Cell Imaging kit (R37601). The LIVE/DEAD Cell Imaging kit is based on a cell-permeable dye (calcein, AM) that stains live, viable cells bright green and a cell-impermeable red marker that only stains dead and dying cells, which are characterized by compromised cell membranes. Labeled U-2 OS cells were treated 1 μM staurosporine and fluorescence images in the FITC and TexasRed channels were acquired every 5 minutes over 14 h on the EVOS Auto Imaging System using a 20x objective. | cell viability, fluorescence microscopy/fluorescence imaging, ion channels, ion flux, ion indicators, live-cell imaging |
| Video | CellTracker Violet reagent and mitosis U-2 OS cells were transduced with CellLight Tubulin-GFP and Cellular Lights Actin-RFP. The following day cells were labeled with 5uM CellTracker Violet BMQC for 30 minutes at 37C in complete media and washed in fresh media. Images were taken every 5 minutes for 16 hours. | cell structure-all, cell tracking, fluorescence microscopy/fluorescence imaging, live-cell imaging |
| Video | Wound healing and cadherin-4 expression Murine glioma cells were transfected with a DsRed-cadherin-4 fusion, which plays a critical role in cell migration during wound repair, or a GFP control plasmid, then mixed with wild-type cells and seeded in a 24-well plate. After scratch wounding, cell migration was followed over a 21 h period on the EVOS Cell Imaging System equipped with an EVOS Onstage Incubator using a 10X phase objective. Images were captured at an interval of about 10 minutes (116 images total). Video courtesy of Paolo Malatesta, PhD., IRCCS Azienda Ospedaliera Universitaria San Martino -- IST, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy. | EVOS, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, wound healing |
| Video | Compilation of live cell imaging videos using Invitrogen fluorescent reagents This video demonstrates novel product brands from Invitrogen for live cell imaging include CellLight targeted fluorescent proteins, CellROX reagents for oxidative stress, CellEvent caspase 3/7 detection reagent, and many more fluorescent dyes and probes. | cell structure-mitochondria, CellEvent, CellLight, CellROX, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress |
| Video | Click-iT Plus TUNEL apoptosis assays The Click-iT Plus TUNEL assay detects apoptotic cells in tissue and cultured cell samples through the use of a small, highly specific labeling moiety and a bright fluorescent dye. After incorporation of the labeling moiety into DNA fragments, detection is achieved through a catalyzed "click" reaction using conditions mild enough to preserve the fluorescent signal from GFP or RFP. | apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging |
| Video | Washing your sample Molecular Probes School of Fluorescence explains the wash step common to many cellular imaging protocols. | fluorescence microscopy/fluorescence imaging, imaging sample preparation |
| Video | Z Stack image of HeLa cells labeled with CellLights reagents A series of images were captured on the EVOS FL Auto Cell Imaging System. Creating a Z-stack from these images allowed the observation of cellular cytoskeletal changes, which can be indicative of the loss of cell health. Methods HeLa cells grown in MatTek 6-well glass bottom culture plates were transduced with CellLights Tubulin-GFP and CellLight Mitochondria-RFP overnight at 37oC. The following day, NucBlue Live reagent (2 drops/mL) was added to the cultures. Cells were then imaged on an EVOS FL Auto Cell Imaging System with 100x oil immersion objective using the Z-stack function. The step size was set using the Nyquist formula and performed at 0.366 μm. | cell health, cell structure-all, CellLight, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging |
| Video | How to site specifically label your antibody using SiteClick technology This video explains how to easily and site specifically label an antibody using an enzymatic and Click chemistry approach. This method can be applied to any intact IgG antibody and requires no antibody engineering or complex methodology. Unlike classic antibody conjugation techniques this breakthrough SiteClick technology allows for antibody conjugation with complete confidence that the label will not directly interfere with the antibody binding domain. | antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, Qdot, western detection |
| Video | Mounting a Coverslip Molecular Probes School of Fluorescence explains step-by-step how to mount a coverslip properly for cellular imaging. Formulations of mounting media that can add favorable properties such as optimizing the refractive index to match that of glass, preventing photobleaching, or preserving samples for long-term storage are widely available. | fluorescence microscopy/fluorescence imaging, imaging sample preparation |
| Video | Detection of oxidative stress with CellROX Green in U2-OS cells U2-OS cells were plated at 200,000 cells per dish on a 35 mm glass bottom dish (MatTek) and cultured overnight. The cells were rinsed once and loaded with 5 µM CellROX Green Reagent (Cat. No. C10444) and 100 nM TMRM in Live Cell Imaging Solution (LCIS, Cat. No. A14291DJ) for 15 minutes at 37 degrees C. After loading, cells were imaged live with no washing every 20 seconds for seventy minutes on the DeltaVision Core inverted microscope at 37 degrees C. Menadione was added to a final concentration of 100 uM after ten minutes of baseline acquisition. The time lapse data shows the loss of signal from TMRM in the red channel as mitochondrial function decreases, concomitant with the onset of a nuclear signal in the green channel as CellRox Green is oxidized and the reagent migrates to the nucleus to generate a fluorogenic response as the active form of the dye binds to DNA. | CellROX, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress |
| Video | Time-lapse of cell migration and apoptosis during angiogenesis Angiogenesis pseudo-tube formation and apoptosis shown with time-lapse imaging. The time-lapse images were captured and assembled using an EVOS FL Auto Imaging System equipped with the EVOS Onstage Incubator. | angiogenesis, apoptosis, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, onstage incubator |
| Video | Mitochondrial dynamics through cell division U2OS cells were transduced with CellLight Mito-RFP and imaged every 5 minutes for 16 hours. Extensive mitochondrial motility is seen throughout mitosis and as the cell regains it's pre mitotic shape following mitosis. | cell structure-mitochondria, CellLight, fluorescence microscopy/fluorescence imaging, live-cell imaging |
| Video | Click-iT Plus EdU Proliferation Assay Measuring DNA synthesis is the most precise way to detect changes in cell proliferation. Image-based proliferation assays generate spatial and temporal results that can not be detected with other methods. This video describes and compares two of the most referenced image-based proliferation technologies. | cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging |
| Video | Time-lapse with Qtracker 655 cell labeling reagent Time-lapse image of live cells labeled with Qtracker reagents. Adherent HeLa cells were incubated with Qtracker VIVID 655 Cell Labeling Kit (Cat. No.Q25021MP) at 10 nM for one hour. Images were collected over 90 min using a Zeiss LSM 710 confocal microscope and are shown pseudo-colored magenta. | fluorescence microscopy/fluorescence imaging, live-cell imaging |
| Video | Tips and tricks: Phospho Immunofluorescence | cell analysis antibodies, cell imaging, fluorescence microscopy/fluorescence imaging, fixed-cell imaging, immunofluorescence (IF) |
| Video | The secret to solving the most common problem in IF experiments | cell analysis antibodies, cell imaging, fluorescence microscopy/fluorescence imaging, fixed-cell imaging, immunofluorescence (IF) |
| Video | Immunohistochemistry—Tips and tricks for weak or no stain Immunohistochemistry application, offers a spatial visualization and location of an antigen in tissue sections | antibodies, brightfield microscopy, colorimetric, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), secondary detection |
| Video | Immunohistochemistry—Tips and tricks for high background Immunohistochemistry application offers a spatial visualization and location of an antigen in tissue sections. This video offers troubleshooting tips for issues falling into the "high background" category for IHC | antibodies, brightfield microscopy, colorimetric, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), secondary detection |
| Video | Beginner's guide to macro writing: Celleste Image Analysis Software | brightfield microscopy, Celleste, fluorescence microscopy/fluorescence imaging, imaging analysis |
| Video | Regions of interest: Celleste Image Analysis Software | brightfield microscopy, Celleste, fluorescence microscopy/fluorescence imaging, imaging analysis |
| Video | Cell and object counting: Celleste Image Analysis Software | brightfield microscopy, Celleste, fluorescence microscopy/fluorescence imaging, imaging analysis |
| Video | Batch processing: Celleste Image Analysis Software | brightfield microscopy, Celleste, fluorescence microscopy/fluorescence imaging, imaging analysis |
| Webinar | Fixed cell imaging—Five steps for publication-quality images With over 40 years dedicated to cell imaging research, we offer long-proven tools and protocols to help confidently create quality cell images the first time. This on demand webinar covers the 5 essential steps to getting great images. | antibodies, blocking, Celleste, dyes, EVOS FL Auto 2.0, fixation, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), sample detection, sample labeling, sample preparation, signal amplification |
| Webinar | Learn to choose the right fluorophore when designing experiments The choice of fluorophore is one of the first important decisions to make in developing an experiment. Fluorophores are compounds that emit light at a specific wavelength when they have been excited at another, lower wavelength. Join our webinar and explore: How to choose the best organic dye for an assay Quantum dots and how they compare to other dyes When to use a phycobiliprotein like R-PE or APC When to use fluorescent proteins like GFP How to choose a suitable dye to match your instrument In addition, we will explore the basic characteristics, strengths, and weaknesses of the various fluorophores to help you choose and develop the best assay for your needs. | antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins |
| Webinar | A practical approach to antibody labeling The growing number of fluorophores available makes labeling your own antibodies a tempting proposition. But with many antibody labeling solutions available, selecting the best option can be a daunting task. In this webinar we will: Provide an overview of our antibody labeling kits Offer guidance on which methods are ideal for specific applications and experiments Provide tips and tricks to optimize your labeling protocol | antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF) |
| Webinar | An introduction to immunofluorescence staining of cultured cells In this webinar, we discuss the steps of an immunofluorescent staining protocol including material list, common variations, and necessary controls. We'll also provide a simple troubleshooting guide and examine how to avoid common pitfalls. Presented by Jason Kilgore, Technical Support Specialist, Thermo Fisher Scientific. | antibodies, antibody labeling,antifades, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF) |
| Webinar | Basic techniques in autophagy research This webinar will introduce you to a series of analytical tools and techniques to help you identify and interrogate key features of autophagy. Topics to be covered include: Tips and tricks for selecting the right tools and achieving the best results Fluorescent proteins and antibodies used to analyze both live and fixed cells Analysis steps using a variety of multiplexing options, with quantitative methods for image analysis or fluorescence intensity measurement | autophagy, cell health, fluorescence microscopy/fluorescence imaging, fluorescent proteins, high content analysis, immunofluorescence (IF) |
| Webinar | The meaning of life at the cellular level: detecting apoptosis with fluorescence In this webinar, Thermo Fisher Scientific scientists will demonstrate several solutions using fluorescent probes to study key components of the apoptotic machinery. The presentation includes: An overview of the features of apoptosis Key parameters that can be measured to assay apoptosis A comprehensive guide to available labeling and detection technologies for apoptosis research Tips and tricks to best implement those technologies | apoptosis, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent proteins |
| Webinar | The meaning of life at the cellular level: Visualizing membrane trafficking Membrane trafficking underlies the acquisition and release of cellular material as well as the transport of macromolecules from the site of cellular synthesis to their functional location. Thermo Fisher Scientific offers a number of solutions for researchers using fluorescent probes to study key components of the endocytotic and secretory pathway. This webinar will: Provide an overview of the processes involved in membrane trafficking, along with strategies to investigate them using fluorescence microscopy Offer a comprehensive guide to available labeling and detection technologies for membrane trafficking research Provide tips and tricks on how to best implement those technologies | endocytosis, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, membrane trafficking, particles |
| Webinar | A comparison of basic immunofluorescent labeling strategies In this free webinar, we will compare different immunofluorescent labeling strategies exploring the pros and cons of each method. You will learn when the use of a direct conjugate is appropriate and when amplification techniques can be utilized. We'll also present a simple decision tree to aid in determining the best method for each situation. | Alexa Fluor, antibodies, antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunocytochemistry (ICC), immunofluorescence (IF) |
| Webinar | Breathing new life into hypoxia research Although the significance of hypoxia in biological processes is well known, creating model systems with accurate control of hypoxic conditions is extremely difficult without access to elaborate systems that allow precise control and maintenance of temperature, humidity, and gases (CO2 and O2) during an experiment. In this webinar, we will discuss: Overview of hypoxia in human diseases Classical methods for setting up hypoxic conditions Novel instruments and reagents for imaging cells in hypoxic conditions | EVOS, fluorescence microscopy/fluorescence imaging, high content analysis, hypoxia, onstage incubator |
| Webinar | The meaning of life at the cellular level: Probing viability with fluorescence This webinar will provide an overview of features of healthy and unhealthy cells as well as describing key parameters that can be measured to assay cell viability. The webinar also offers a comprehensive guide to available labeling and detection technologies for cell health research as well as tips and tricks on how to best use them. | cell health, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, viability |
| Webinar | Introduction to basic cytoskeleton labeling and detection The cytoskeleton is a key component of mammalian cells, providing the framework for cell migration and intracellular transport, furthermore the cytoskeleton regulates cell size and shape as well as important processes such as mitosis and endocytosis. We offer a number of solutions for researchers using fluorescent probes to study the cytoskeleton. This webinar will provide an overview of the structures that comprise the cytoskeleton and important experimental parameters. The webinar also offers a comprehensive guide to available labeling and detection technologies for cytoskeletal research as well as tips and tricks on how to best use them. These tools include those for live-cell imaging fluorescent dyes, antibodies and the BacMam gene delivery platform. | cell structure-actin, cytoskeleton, endocytosis, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins |
| Webinar | From the hood to the microscope; Revolutionizing cell-based imaging Join us for an interactive, educational webinar that showcases some of the latest advancements in cell-based research and imaging. Learn as we explore how to obtain superior results through the careful selection of reagents and the optimization of your imaging workflow— from growing cells on suitable surfaces through to image capture. Particular attention will be given to growing and monitoring cells for imaging, fluorescent labeling of live cells, critical considerations for time-lapse imaging and optimizing live cell imaging. Topics include: Choose a suitable imaging culture vessel for studying cell growth and viability using bright-field microscopy Label cells to maximize signal-to-noise for fluorescence imaging Prepare culture conditions and capture images without losing temporal data for time-lapse imaging Achieve hypoxic culture conditions by modifying gas conditions or employing cell spheroid cultures | cell health, EVOS, FLoid, fluorescence microscopy/fluorescence imaging, hypoxia, live-cell imaging, onstage incubator |
For Research Use Only. Not for use in diagnostic procedures.