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Ion Indicators and Ion Channels Information
Learn about assays and techniques for detecting and monitoring ion channels and ion flux using fluorescent ion indicators by flow cytometry, imaging microscopy, high-content analysis and microplate assays. Topics include calcium and magnesium indicators, as well probes for potassium channel flux.
Ion indicators and ion channels features
BioProbes article
FluxOR II Green Potassium Ion Channel Assay—Next-generation detection of potassium ion flux
Potassium channels are ion-selective protein pores that span the cell’s plasma membrane and serve to establish and regulate membrane potential. In excitable cells such as neurons and myocytes, these channels function both to shape the action potential and to reset the cell’s resting membrane potential. The Invitrogen FluxOR II Green Potassium Ion Channel Assay is the newest tool for high-throughput detection of potassium ion channel and transporter activities.
Molecular Probes Handbook
Technical Note
Loading and Calibration of Intracellular Ion Indicators—Note 19.1
This article provides an overview of what you need to know to load ion indicators into living cells and how to calibrate your system to get the most out of the data.
Ion indicators and ion channels learning resources
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| Type | Title | Categories |
|---|---|---|
| BioProbes articles (Issues 50– present day) | BioProbes Journal of Cell Biology Application | cell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification |
| Molecular Probes Handbook | MAXC Computer Program for calculating Free Ca2+ concentrations—Note 19.2 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Loading and calibration of intracellular ion indicators—Note 19.1 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Calcium regulation—Section 17.2 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Probes for ion channels and carriers—Section 16.3 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Probes useful at near-neutral pH—Section 20.2 | BCECF, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, pH detection, pH indicators, SNARF |
| Molecular Probes Handbook | Probes useful at acidic pH—Section 20.3 | flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo |
| Molecular Probes Handbook | pH indicator conjugates—Section 20.4 | flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo |
| Molecular Probes Handbook | Overview of pH indicators—Section 20.1 | flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo |
| Molecular Probes Handbook | Fluorescent Na+ and K+ indicators—Section 21.1 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Detecting chloride, phosphate, nitrite and other anions—Section 21.2 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Introduction to Ca2+ measurements with fluorescent indicators—Section 19.1 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Fluorescent Mg2+ indicators—Section 19.6 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Fluorescent indicators for Zn2+ and other metal ions—Section 19.7 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Fluorescent Ca2+ indicators excited with visible light—Section 19.3 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Fluorescent Ca2+ indicators excited with UV light—Section 19.2 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Fluorescent Ca2+ indicator conjugates—Section 19.4 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Chelators, calibration buffers, ionophores and cell-loading reagents—Section 19.8 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Protein-based Ca2+ indicators—Section 19.5 | calcium flux, fluorescent dyes, ion indicators |
| Scientific poster (2008) | Living molecular probes for the next generation | BacMam technology, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, ion flux/ion channels, ion indicators, live-cell imaging |
| Scientific poster (2008) | Cell-based potassium ion channel screening with the FluxOR Assay | BacMam technology, cell counting, Countess, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader |
| Scientific poster (2008) | Improving downstream results with automated cell counting | automated cell counter, BacMam technology, cell counting, cell expansion, cell isolation, Countess, fluorescent dyes, hemocytometer, ion flux/ion channels, microplate reader |
| Scientific poster (2008) | FluxOR, a universal potassium channel assay: Screening BacMam-delivered and stably expressed targets | BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader |
| Scientific poster (2009) | Cell-based potassium ion channel screening with BacMam using the FluxOR Assay | BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader |
| Scientific poster (2010) | A new generation of cellular imaging probes for neurosciences research | autophagy, BacMam technology, CellLight, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels, ion indicators |
| Scientific poster (2010) | BacMam gene expression for functional and imaging applications | BacMam technology, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels |
| Video | LIVE DEAD Cell Imaging kit on the EVOS Auto Imaging System Time course of cell death visualized using the LIVE/DEAD Cell Imaging kit (R37601). The LIVE/DEAD Cell Imaging kit is based on a cell-permeable dye (calcein, AM) that stains live, viable cells bright green and a cell-impermeable red marker that only stains dead and dying cells, which are characterized by compromised cell membranes. Labeled U-2 OS cells were treated 1 μM staurosporine and fluorescence images in the FITC and TexasRed channels were acquired every 5 minutes over 14 h on the EVOS Auto Imaging System using a 20x objective. | cell viability, fluorescence microscopy/fluorescence imaging, ion channels, ion flux, ion indicators, live-cell imaging |
For Research Use Only. Not for use in diagnostic procedures.