Mouse B Cell Isolation

	Highest purity for molecular assays		Untouched -most physiological relevant cells
	Dynabeads® Mouse Pan B (B220)		Dynabeads® Mouse CD43 (Untouched cells)
Isolation method	Depletion or positive isolation		Negative isolation
Starting samples	Any sample (whole blood, spleen, lymph node)		Spleen, lymph node cells*
# cells processed	1–2 x 109 cellls		1 x 109 cells
Purity	High >90%		Higher >95%
Yield	Highest >95%		High >70%
Viability	>90%		>90%
Isolation time	~30 min		~50 min
Isolation temperature	cold		cold
Applications	Depletion of unwanted B cells or Positive isolation for molecular: isolation of proteins & nucleic acids		Cellular applications for physiological studies (functional studies, adoptive transfer, flow analysis or further sorting)
Bead-free target cells	No		Yes
Compatible with flow cytometry /flow sorting	No		Yes
Shelf life from production date	3 years		2 years
Required materials	Magnets		Magnets
	Order Now		Order Now

* Lyse the red cells prior to use to improve the purity