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Flow Cytometry Educational Videos & Webinars
Technical and educational videos for flow cytometry topics
These videos, tutorials and webinars are designed to provide technical and education information regarding flow cytometers as well as flow cytometry research applications and techniques.
Learn more about the Invitrogen Attune Flow Cytometers
Learn more about the Invitrogen Bigfoot Spectral Cell Sorter
Featured videos
Flow cytometry as you have never seen it before
This webinar addresses potential applications of integrating morphology analysis with standard flow cytometry, with specific focus on cancer research, such as those involving circulating tumor cells, cell mediated cytotoxicity, and apoptosis detection as you have never seen before.
Exploring the five pillars of cell sorting: viability, recovery, efficiency, purity, and proliferation
Learn how to interpret post sort viability, recovery, efficiency, purity, and proliferation using the Bigfoot Spectral Cell Sorter.
Identifying and sorting elusive cells using a high dimensional spectral panel
Using a 28-color mouse immunophenotyping panel we demonstrate the spectral unmixing and sorting capabilities of the Bigfoot Spectral Cell Sorter to resolve fluorescent signals accurately and precisely while sorting six ways simultaneously with high efficiency and purity.
Benefits of combining morphometric data with flow cytometry: A panel discussion
A panel of experts discuss the benefits of imaging data in flow cytometry experiments, enabled with the Attune CytPix flow cytometer.
Automated image analysis reduces user-to-user variability in flow cytometry gating strategies
This webinar describes how automated image analysis for morphometric parameters on the Attune CytPix flow cytometer enables reduced user-derived variability in flow cytometry experiments, enabling higher precision in your data.
Simplifying GxP Compliance from Acquisition to Final Result with the Attune Cytometric Software and FCS Express
With the release of 21 CFR Part 11 compliance ready software for Attune flow cytometers and De Novo Software’s continued support for compliance, scientists now have a complete end-to-end GxP flow cytometry acquisition and analysis.
Sample characterization of complex tissues on Attune CytPix Flow Cytometer
Herve Luche of CIPHE presents data on characterization of complex tissues using the Attune CytPix Flow Cytometer.
Brightfield Imaging-enabled flow cytometry with the Attune CytPix
Learn how the imaging-enhanced Attune CytPix flow cytometer is being used by the Amsterdam University Medical Centers Microscopy & Cytometry Core Facility to help users optimize their flow cytometry gating strategy and gain deeper insight into their sample. Using the imaging feature of the Attune CytPix, operators can quality control their flow cytometry samples and adjust gates to accurately exclude aggregates, coincident events, debris, and unwanted cells. Presented by Cora Chadick.
Integrating spatial resolution into flow cytometric analysis
Presented by R&D scientist Heaven Roberts, this webinar will further your understanding of how images can present novel data for cytometrists seeking even greater depth of insight into their samples and preparations— as well as how the Attune CytPix Flow Cytometer helps deliver enhanced imaging for your application.
Easily upgrade your flow cytometry data with brightfield images using the Attune CytPix Flow Cytometer
Join Dr. Cora Chadick, as she demonstrates how easy it is to start collecting imaging flow cytometry data using the new Attune CytPix Flow Cytometer. Watch as she walks through the simple workflow, instrument setup, parameter optimization, data collection, and data analysis steps.
Introducing the Attune CytPix Flow Cytometer: "Seeing is believing"
The Attune CytPix Flow Cytometer is an advanced cell analyzer that combines acoustic focusing fluidics for high sensitivity and high throughput with a high-speed camera. Watch the CYTO 2021 presentation from product manager Kate Alford and lead engineer Greg Kaduchack.
Flow cytometry as a microbiology tool in the development of next generation diagnostics
Learn how Dr. Inglis and his team have harnessed the speed and precision of the Attune NxT to innovate a new generation of rapid microbiology research tools. They demonstrate how these tools function, how they can be used to accelerate laboratory workflows, and how the lessons learned can be applied to new and emerging challenges in the microbiology laboratory.
Endpoint assays for the characterization of rare and therapy-resistant cells in cancer
Learn from Jordi Petriz, from Josep Carreras Leukaemia Research Institute about using flow cytometry to detect and analyze immune cells using a no-wash no-lyse strategy.
Infection with SARS-CoV-2 is associated with complex pathological conditions
One of these conditions observed in samples with severe SARS-CoV-2 infection is the progressive increase of immune-mediated inflammation, defined as a “cytokine storm.” Dr. Sara De Biasi describes the abnormally increased levels of cytokines in plasma from samples infected by SARS-CoV-2, and how flow cytometry has helped her investigate the immune system and cells producing cytokines in samples affected by coronavirus .
Single-cell analyses of checkpoint-inhibition-induced myocarditis: implications into mechanisms of severe immune-related adverse events
Learn how Dr Justin Balko, Assoc Professor, Vanderbilt University, Department of Medicine, utilized flow cytometry to study immune checkpoint inhibitors as possible treatments in myocarditis associated with cancer.
Learn how flow cytometry streamlines and enhances the CRISPR gene editing workflow
The advent of CRISPR has radically changed the face and pace of biological research and has unlocked unprecedented potential in the study of functional genomics, target identification, and gene therapy. Using flow cytometry in the gene editing workflow can both expedite and augment the process, while enabling a more in-depth and meaningful analysis of engineered cells.
Panel Design for Multi-Parameter Flow Cytometry
Join FAS Carol Oxford on panel design. Learn the value of including the correct controls and reagent titration. Understand the importance of fluorescence spillover and finding your fluorescence spectral balance.
The complex pharmacology of T-cell CARs
In this webinar, Charles Prussak, PharmD, PhD., Director of the Cell Therapy Translational Laboratory (CTTL), UCSD, discusses the generation CAR-T therapies, toxicities that are most commonly observed when employing CD-19 directed CAR-T therapies, and the next generation of CAR-T cells being created and clinically tested for the treatment of both hematological and solid tumor malignancies.
How recent technological advances in flow cytometry instrumentation are enabling faster throughput
This webinar presented by J. Paul Robinson from Purdue University, focuses on advances in the Invitrogen Attune NxT Flow Cytometer and outlines where the technology within the Attune NxT system fits in current and future applications.
CYToMORE 2016—Advances in cytometry
The 6th Modena Advanced Course in Cytometry (CYToMORE), was aimed at researchers who wanted to improve their flow cytometry knowledge and learn new applications. Organized in collaboration with the Italian Society of Immunology, Clinical Immunology and Allergology (SIICA) and led by teachers who are among the top international experts in the field, 2016 was a huge success. In this presentation, Victor Appay, Faculté de Médecine Pierre et Marie Curie, at Pitié-Salpêtrière, France discusses his work on “Assessing immunological aging using flow cytometry”.
Tutorial—Introduction to flow cytometry
In this tutorial, see the basics of how a flow cytometer works, how scattered light and fluorescence are detected by a flow cytometer, and how the resulting data can be analyzed.
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| Type | Title and description | Categories |
|---|---|---|
| CYTO 2022; webinar presentation | Integrating spatial resolution into flow cytometric analysis Presented by Heaven Roberts, R&D Scientist, Thermo Fisher Scientific | flow cytometry, imaging, cell analysis |
| CYTO 2021 poster presentation | A Rapid Flow Virometric Test for Detection of SARS-CoV-2 Particles Presented by Laura G. Rico, Josep Carreras Leukaemia Research Institute (IJC), ICO-Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona, Spain | flow cytometry, COVID-19, Coronavirus disease 2019 |
| CYTO 2021 poster presentation | Erythrolytic Buffers Have a Detrimental Impact in Flow Cytometric Detrimental Impact in Flow Cytometric Dendritic Cell Detection Presented by Laura G. Rico, Josep Carreras Leukaemia Research Institute (IJC), ICO-Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona, Spain | flow cytometry, dendritic cell (DC) |
| CYToMORE lecture video (2016) | ...and on the 8th day, God created cytometry Presented by J. Paul Robinson, Purdue University, USA | flow cytometers, flow cytometry, history, technology developments |
| CYToMORE lecture video (2016) | Assessing immunological aging using flow cytometry Presented by Victor Appay, Faculté de Médecine Pierre et Marie Curie, Pitié-Salpêtrière, France | flow cytometry, immunology, immunophenotyping |
| CYToMORE lecture video (2016) | Asymptomatic but threatening: dissecting the consequences of cytomegalovirus infection Presented by Anis Larbi, Singapore Immunology Network – SigN, Singapore | flow cytometry assays, immunology |
| CYToMORE lecture video (2016) | Cancer stem cells and multi-drug resistance by flow cytometry Presented by Jordi Petriz, Josep Carreras Leukaemia Research Institute, IJC, Spain | cancer, flow cytometry, multidrug resistance (MDR), stem cell research |
| CYToMORE lecture video (2016) | Cytometric analysis of cytokines and transcription factors Presented by Hyun-Dong Chang, Deutsches Rheuma-Forschungszentrum, Berlin, Germany | cell signaling, flow cytometry, immunophenotyping |
| CYToMORE lecture video (2016) | Cytometry of immunity Presented by Andreas Radbruch, Deutsches Rheuma-Forschungszentrum Berlin, Germany | flow cytometry, immunology |
| CYToMORE lecture video (2016) | Cytomic analysis of oxidative stress Presented by J. Enrique O’Connor, University of Valencia, Spain | flow cytometry, oxidative stress |
| CYToMORE lecture video (2016) | High throughput and high content flow cytometry Presented by J. Paul Robinson, Purdue University, USA | Attune NxT, flow cytometer, high content flow cytometry, high throughput screening |
| CYToMORE lecture video (2016) | Heterogeneity of tumour-infiltrating leukocytes: implications for adoptive cell transfer immunotherapy Presented by Enrico Lugli, Humanitas Clinical and Research Center, Italy | flow cytometry, immunology, immunophenotyping |
| CYToMORE lecture video (2016) | Host-microbe interaction at mucosal surfaces Presented by Maria Rescigno, Istituto Europeo di Oncologia – IEO, Italy | flow cytometry, immunology, microbiology |
| CYToMORE lecture video (2016) | Mass cytometry in the phenomics era: an essential tool for gene/function studies Presented by Herve Luche, Centre d’Immunophénomique - CIPHE, France | mass cytometry, immunology, immunophenotyping |
| CYToMORE lecture video (2016) | Microenvironment cytometry Presented by Paul Smith, Cardiff University, UK | cancer, cell cycle, flow cytometry, tumor microenvironments, drug discovery |
| CYToMORE lecture video (2016) | Novel reagent solutions for flow cytometry Presented by Jolene Bradford, Thermo Fisher Scientific, USA | Attune NxT, flow cytometry assays |
| CYToMORE lecture video (2016) | Rare cells analysis: from bench to clinics Presented by Andrea Cossarizza, University of Modena and Reggio Emilia, Italy | Attune NxT, flow cytometer, rare cell detection, rare event detection |
| CYToMORE lecture video (2016) | RNA flow cytometry Presented by Paul Wallace, Roswell Park Cancer Institute, USA | flow cytometry, in situ hybridization, RNA |
| CYToMORE lecture video (2016) | Software tutorial: what’s new Presented by Carmen Gondhalekar, Purdue University, USA | Attune NxT, flow cytometer, software |
| CYToMORE lecture video (2016) | Tuning acoustic forces for flow cytometry Presented by Jolene Bradford, Thermo Fisher Scientific, USA | Attune NxT, flow cytometer |
| How-to video | Tutorial: Labeling a purified antibody Educational video on how to label your next antibody for imaging or fluorescence. Walk through the tutorial as Molecular Probes scientists demonstrate the protocol—including all the tips and tricks you'll want to know about for your next antibody labeling experiment. The video features Judie showing Curtis, a chemistry graduate student, how to label his monoclonal antibodies while saving time and maximizing yield. | antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| How-to video | How to site specifically label your antibody using SiteClick technology This video explains how to easily and site specifically label an antibody using an enzymatic and Click chemistry approach. This method can be applied to any intact IgG antibody and requires no antibody engineering or complex methodology. Unlike classic antibody conjugation techniques this breakthrough SiteClick technology allows for antibody conjugation with complete confidence that the label will not directly interfere with the antibody binding domain. | antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, Qdot, western detection |
| Technology video | Click-iT Plus EdU Proliferation Assay Measuring DNA synthesis is the most precise way to detect changes in cell proliferation. Image-based proliferation assays generate spatial and temporal results that can not be detected with other methods. This video describes and compares two of the most referenced image-based proliferation technologies. | cell proliferation, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging |
| Technology video | The Two Worlds of Cell Separation | cell isolation, immunology |
| Tutorial video | Molecular Probes Tutorial Series—Analyzing Flow Cytometry Data | flow cytometer calibration, flow cytometry |
| Tutorial video | Molecular Probes Tutorial Series—Anatomy of Fluorescence Spectra | absorbance, emission, excitation, spectra |
| Tutorial video | Molecular Probes Tutorial Series—Introduction to Flow Cytometry | flow cytometer calibration, multicolor flow cytometry |
| Tutorial video | Molecular Probes Tutorial Series—Introduction to Fluorescence | flow cytometry, fluorescence imaging |
| Tutorial video | Molecular Probes Tutorial Series—Overview of Filters and Light Sources | emission, excitation, filters, light cubes, light sources |
| Video (Meet the Inventor) | Attune NxT Flow Cytometer: Small in size, Big in Performance Greg Kaduchak (Engineering Director Flow Cytometry, Thermo Fisher Scientific) gives an overview of the design and capabilities of the acoustic-assisted cytometry that's at the core Attune NxT Flow Cytometer. | Attune NxT |
| Video (Meet the Inventor) | Attune NxT Flow Cytometer: Software Jolene Bradford (Associate Director Flow Cytometry, Thermo Fisher Scientific) describes how the software of the Attune NxT Flow Cytometer helps users automate routine maintenance tasks, instrument performance alerts, and system cleaning. | Attune NxT |
| Video (Meet the Inventor) | Attune NxT Flow Cytometer: Fluidics Jolene Bradford (Associate Director Flow Cytometry, Thermo Fisher Scientific) speaks about how the fluidics system within the Attune NxT Flow Cytometer helps users minimize sample usage and avoid running buffer reservoirs dry. | Attune NxT |
| Video (Meet the Inventor) | Attune NxT Flow Cytometer: Optics Wesley Smith (Mechanical Engineer Flow Cytometry, Thermo Fisher Scientific) discusses the variety of laser configurations available on the Attune NxT Flow Cytometer, designed to support more advanced experiments and growing lab needs. | Attune NxT |
| Video (Meet the Inventor) | Attune NxT Flow Cytometer: Acoustics Michael Ward (Engineering Lead Flow Cytometry, Thermo Fisher Scientific) speaks about how the design of the Attune NxT Flow Cytometer offers smooth sample introduction, broadly variable volumetric flow rates, and robust performance for improved accuracy in cell analysis. | Attune NxT |
| Webinar | Introducing flow cytometry-assisted antimicrobial susceptibility testing (FAST) Learn how Dr. Tim Inglis, a clinical microbiologist at the University of Western Australia in Perth, leveraged the speed, accuracy, and broad drug/bug applicability of the Invitrogen Attune NxT Flow Cytometer to get same-day susceptibility results when combined with machine learning-based data analysis. Categories: flow cytometry, microbiology, flow cytometry-assisted antimicrobial susceptibility testing (FAST). | flow cytometry, microbiology, flow cytometry-assisted antimicrobial susceptibility testing (FAST) |
| Webinar | The Role of Acoustic Cytometry in Cancer Immunotherapy and Leukemia Learn from Jordi Petriz, PhD from Josep Carreras Leukaemia Research Institute about using flow cytometry to detect and analyze immune cells. In this video he discusses the use of flow cytometry to create the next generation of immunotherapy in hematological cancers. | flow cytometry, cancer, Attune NxT |
| Webinar | Novel Fluorescent Assays and Labeling Strategies for Flow Cytometry Learn about cell proliferation, apoptosis, and viability assays from Jolene Bradford | Flow cytometry, flow reagents |
| Webinar | A practical approach to antibody labeling The growing number of fluorophores available makes labeling your own antibodies a tempting proposition. But with many antibody labeling solutions available, selecting the best option can be a daunting task. In this webinar we will:
| antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF) |
| Webinar | An introduction to flow cytometric analysis, Part 1: Cell proliferation analysis In this 2-part series, we will give an overview of tools and techniques using Invitrogen reagents for flow cytometric analysis of cell proliferation, viability, vitality, and apoptosis. | apoptosis, cell cycle, cell proliferation, multicolor flow cytometry, viability |
| Webinar | An introduction to flow cytometric analysis, Part 2: Cell viability and apoptosis analysis In this webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells. Changes introduced by apoptosis can be tested with numerous assays measuring membrane structure, mitochondrial function, metabolism, caspase activity, membrane integrity, and DNA fragmentation. We will also discuss dead cell identification using traditional impermeant nucleic acid dyes. | apoptosis, cell cycle, cell proliferation, multicolor flow cytometry, viability |
| Webinar | Analysis of surface antigens on exosomes using the Attune NxT Flow Cytometer Join Steve McClellan as he shares how he analyzes individual exosomes for the expression of a variety of surface markers using multi-color flow cytometry. | Attune NxT, multicolor flow cytometry |
| Webinar | Basics of flow cytometry, Part I: Gating and data analysis An introduction to fluorescence, this presentation covers the following topics: the principle of the flow cytometry platform, the basic components of a flow cytometer, how to interpret a dye excitation/emission spectrum, how data is displayed, basic gating demonstration, and common statistics and terminology used in flow cytometry. | flow cytometry data, flow cytometry set up, multicolor flow cytometry |
| Webinar | Basics of flow cytometry, Part II: Compensation This presentation provides an overview of basic fluorochromes used in flow cytometry. Topic includes: the principle of compensation, how to perform compensation, the types of controls recommended and their use, basic strategies for designing a flow experiment, and data presentation. | flow cytometry data, flow cytometry set up, multicolor flow cytometry |
| Webinar | Basics of multi color flow cytometry panel design With the proliferation of new fluorescent dyes, as well as instruments that can detect 18 or more parameters multicolor flow cytometry has become more popular and more accessible than ever. This webinar presented by Dr. Holden T. Maecker at Stanford University will discuss the caveats of good panel design, including:
| antibodies, compensation, multicolor flow cytometry, |
| Webinar | DNA content cell cycle analysis using flow cytometry Find out how careful acquisition and methodical preparation contribute to accurate and consistent DNA analysis. In this webinar we’ll discuss:
| cell counting, cell cycle, cell proliferation, flow cytometry |
| Webinar | Flow cytometry in microbiological research In recent years the application of flow cytometry in microbiological research has expanded from detection and quantification of organisms to more complex studies including analysis of host-microbe interactions and detailed spatial and temporal analysis of microbial metabolism in different environments. During this webinar we will discuss how the multi-parametric nature of flow cytometry can be applied to microbiology and the advantages of using this application over traditional microbiological methods. | microbiology, multicolor flow cytometry, viability |
| Webinar | How recent technological advances in flow cytometry instrumentation are enabling faster throughput This webinar presented by J. Paul Robinson at Purdue University focuses on advances in the Invitrogen Attune NxT Flow Cytometer and outline where the technology within the Attune NxT instrument fits in current and future applications. | Attune NxT, cell cycle, multicolor flow cytometry |
| Webinar | Learn to choose the right fluorophore when designing experiments The choice of fluorophore is one of the first important decisions to make in developing an experiment. Fluorophores are compounds that emit light at a specific wavelength when they have been excited at another, lower wavelength. Join our webinar and explore:
| antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins |
| Webinar | Making polychromatic flow cytometry easy Join Grace Chojnowski, Flow Cytometry and Imaging Facility Manager at QIMR Berghofer Medical Research Institute as she discusses how to make polychromatic flow cytometry easy after instrument standardization and verification for different fluorochromes on different flow cytometers and cell sorters. | Attune NxT, multicolor flow cytometry |
| Webinar | Multiparameter cell cycle analysis Join James W Jacobburger as he shares how to get better cell recovery and definition of cell cycle compartments. This webinar focuses on multiparametric cell cycle analysis of DNA and specific epitopes using a “washless” staining assay to minimize sample handling. | Attune NxT, multicolor flow cytometry |
| Webinar | The Complex Pharmacology of T-Cell CARs In this webinar, Charles Prussak, PharmD, PhD., Director of the Cell Therapy Translational Laboratory (CTTL), UCSD, discusses the generation CAR-T therapies, toxicities that are most commonly observed when employing CD-19 directed CAR-T therapies, and the next generation of CAR-T cells being created and clinically tested for the treatment of both hematological and solid tumor malignancies. | flow cytometry, immuno-oncology |
| Webinar | Panel Design for Multi-Parameter Flow Cytometry Join FAS Carol Oxford on panel design. Learn the value of including the correct controls and reagent titration. Understand the importance of fluorescence spillover and finding your fluorescence spectral balance. | multicolor flow cytometry, Attune NxT Flow Cytometer |
| Webinar | Identify Cancer Stem Cells in Primary Tumors and in Circulation using Flow Cytometry Join Bruno Sainz as he discusses the advancements in the detection of cancer stem cells (CSC) in primary tumors and in circulation using acoustic flow cytometry. | flow cytometry |
| Webinar | Analyzing your CRISPR gene editing efficiencies using flow cytometry In this webinar, Natasha Roark, scientist at Thermo Fisher Scientific, discusses CRISPR gene editing technology and its role in flow cytometry editing efficiencies. | flow cytometry |
| Webinar | Advancing immuno-oncology discovery and development with flow cytometry In this webinar, Chris Langsdorf, scientist at Thermo Fisher Scientific, discusses how flow cytometry can effectively be employed in research efforts aimed at advancing the discovery and development of various immunotherapy strategies. | flow cytometry |
| Webinars (ongoing) | Protein and cell analysis upcoming educational webinars See our upcoming webinars and see what is now available on demand in our virtual learning platform. | education |