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Cell line data table of contents
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Nunclon surface performance data
We’ve tested the most-used cell lines on Thermo Scientific Nunclon Delta, Supra and Sphera cell culture plastics side-by-side with the leading competitor to validate Nunc cell culture plastics’ place in your cell culture hood.
Please note: We are adding data for more cells all the time.
A431 doubling times and gene expression
A431 cells grown on Nunc Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
A431 morphology with Nunclon Delta surfaces
Below are representative brightfield images of A431 cells grown on Nunclon Delta surfaces (left) and Corning® tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
A431 cell viability and growth with Nunclon Delta surfaces
- Graph (A) shows observations on mean cell viability of A431 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
- Graph (B) shows mean population doubling time of A431 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
A431 gene expression with Nunclon Delta surfaces
The below image describes A431 cells grown on Nunclon Delta plates or Corning® TC surfaces for at least 10 passage doublings were assayed for cell health with respect to expression of two common endogenous genes, EGFR (epidermal growth factor receptor) and VEFGA (vascular endothelial growth factor alpha). The normalized relative gene expression of cells grown on Corning TC surface with respect to Nunclon Delta surfaces surface is shown on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
Please note: For the above A431 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco IMDM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
A549 doubling times and EGFR activation
A549 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
A549 morphology with Nunclon Delta surfaces
Below are representative brightfield images of A549 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
A549 cell viability and growth with Nunclon Delta surfaces
- Graph (A) shows observations on mean cell viability of A549 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
- Graph (B) shows mean population doubling time of A549 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
A549 EGFR activation with Nunclon Delta surfaces
A549 cells grown on Nunclon Delta surfaces (Nunc) or Corning TC (Corning) surfaces as well as those switched to Nunclon Delta surfaces (S to ND) surface, were treated with epidermal growth factor (EGF, 200 ng/mL) for 10 minutes. Total cell lysates (30 mg) were subjected to western blot analysis for EGFR phosphorylation using anti-phospho-EGFR (Tyr1086) rabbit polyclonal antibody 0.5 µg/mL. Total EGFR and GAPDH were used as experimental and normalization controls respectively.
Please note: For the above A549 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco IMDM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
Caco-2 doubling times, morphology, and cell attachment
Caco-2 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
Caco-2 morphology with Nunclon Delta surfaces
Below are representative brightfield images of Caco-2 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
Caco-2 cell viability and growth with Nunclon Delta surfaces
- Graph (A) shows observations on mean cell viability of Caco-2 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
- Graph (B) shows mean population doubling time of Caco-2 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
Caco-2 cell attachment with Nunclon Delta surfaces
As an extension of cell attachment and growth, Caco-2 cells grown on Nunclon Delta surfaces (left) and Corning® TC surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface (right) were stained for the tight junction protein ZO-3, shown in green. The nucleus was stained with DAPI (shown in blue). No morphological difference was observed between the surfaces. Cells were grown on 6-well plates and stained on the same surface. Images were captured using EVOS M5000 imaging system under 20X magnification and cropped using the same aspect ratio for better visualization.
Please note: For the above Caco-2 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 20% Gibco FBS, and 0.5% Penicillin Streptomycin.
CHO-K1 doubling times, morphology, and transfection efficiency
CHO-K1 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
CHO-K1 morphology with Nunclon Delta surfaces
Below are representative brightfield images of CHO-K1 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
CHO-K1 cell viability and growth with Nunclon Delta surfaces
- Graph (A) shows observations on mean cell viability of CHO-K1 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
- Graph (B) shows mean population doubling time of CHO-K1 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
CHO-K1 transfection efficiency with Nunclon Delta surfaces
CHO-K1 cells seeded on Nunclon Delta surfaces and Corning TC surface (6-well plates) and transfected with mCherry using Neon Transfection System. Representative images of live cells were acquired 24 hours post transfection.
Please note: Flow cytometry evaluation of transfection efficiency of mCherry in CHO-K1 cells: 10,000 events were captured per sample. UT=Untransfected cells, T=Transfected cells. n=2.
Please note: For the above CHO-K1 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
Cos-7 doubling times and morphology
Cos-7 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
Cos-7 morphology with Nunclon Delta surfaces
Below are representative brightfield images of Cos-7 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
Cos-7 cell viability and growth with Nunclon Delta surfaces
- Graph (A) shows observations on mean cell viability of Cos-7 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis
- Graph (B) shows mean population doubling time of Cos-7 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
Please note: For the above Cos-7 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
HEK293 doubling times, morphology, and transfection efficiency
HEK293 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
HEK293 morphology with Nunclon Delta surfaces
Below are representative brightfield images of HEK293 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
HEK293 cell viability and growth with Nunclon Delta surfaces
- Graph (A) shows observations on mean cell viability of HEK293 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
- Graph (B) shows mean population doubling time of HEK293 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
HEK293 transfection efficiency with Nunclon Delta surfaces
HEK293 cells seeded on Nunclon Delta surfaces or Corning TC surfaces and transfected with mCherry using Lipofectamine 3000 transfection reagent. Images of live cells were acquired 24 hours post transfection.
Please note: Flow cytometry evaluation of transfection efficiency of mCherry in HEK293 cells: 10,000 events were captured per sample. UT=Untransfected cells, T=Transfected cells. n=2.
Please note: For the above HEK293 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
HeLa doubling times, morphology, and transfection efficiency
HeLa cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
HeLa morphology with Nunclon Delta surfaces
Below are representative brightfield images of HeLa cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
HeLa cell viability and growth with Nunclon Delta surfaces
- Graph (A) shows observations on mean cell viability of HeLa cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
- Graph (B) shows mean population doubling time of HeLa cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
HeLa transfection efficiency with Nunclon Delta surfaces
HeLa cells seeded on Nunclon Delta surfaces or Corning TC surfaces (6-well plates) and transfected with mCherry using Lipofectamine 3000 transfection reagent. Images of live cells were acquired 24 hours post transfection.
Please note: Flow cytometry evaluation of transfection efficiency of mCherry in HeLa cells: 10,000 events were captured per sample. UT=Untransfected cells, T=Transfected cells. n=2.
Please note: For the above HeLa Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
HepG2 doubling times, morphology, and gene expression
HepG2 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
HepG2 morphology with Nunclon Delta surfaces
Below are representative brightfield images of HepG2 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
HepG2 cell viability and growth with Nunclon Delta surfaces
- Graph (A) shows observations on mean cell viability of HepG2 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
- Graph (B) shows mean population doubling time of HepG2 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
HepG2 gene expression with Nunclon Delta surfaces
HepG2 cells grown on Nunclon Delta surfaces or Corning TC surfaces for at least 10 passage doublings were assayed for cell health with respect to expression of two common endogenous genes, SLCO2B1 (solute carrier organic anion transporter family member 2B1) and ABCC6 (multidrug resistance associated protein 6). The normalized relative gene expression of cells grown on Corning TC surface with respect to Nunclon Delta surfaces surface is shown on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
Please note: For the above HepG2 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
HT-29 doubling times, morphology, and protein expression
HT-29 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
HT-29 morphology with Nunclon Delta surfaces
Below are representative brightfield images of HT-29 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
HT-29 cell viability and growth with Nunclon Delta surfaces
- Graph (A) shows observations on mean cell viability of HT-29 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
- Graph (B) shows mean population doubling time of HT-29 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
HT-29 protein expression with Nunclon Delta surfaces
- HT-29 cells grown on Nunclon Delta surfaces (left panel) and Corning TC surfaces (right panel) 6-well plates were fixed on the plate and stained for the junction protein ZO-3 using ABfinity Anti-ZO-3 Recombinant Rabbit Oligoclonal Antibody (5 mg/mL) and detected using goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor 488 conjugate (1:2,000). Panel a shows representative cells that were stained for detection and localization of ZO-3 protein (green), Panel b represents cytoskeletal F-actin staining using Alexa Fluor 555 Rhodamine Phalloidin (1:300). Panel c is stained for nuclei (blue) using Hoechst 33342 (1:2,000). Panel d is a composite image of Panels a, b and c demonstrating membrane localization of ZO-3. Scale bar=100 μm.
- As an extension of cell growth and confluency, ZO-3 expression was further detected by western blot at days 2 and 6 of HT-29 cells grown on Nunclon Delta surfaces (Nunc) or Corning TC surfaces (Corning) within 100 mm dishes, as well as those switched to Nunclon Delta surfaces surface (S to ND). The blot was probed with 1 µg/mL of ZO-3 antibody. A 140 kDa band corresponding to ZO-3 was observed across all lanes. Additionally, a 100 kDa band corresponding to ZO-3 isoform was specifically expressed in confluent cells (6 days).
Please note: For the above HT-29 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco IMDM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
MCF-7 doubling times, morphology, and surface marker expression
MCF-7 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
MCF-7 morphology with Nunclon Delta surfaces
Below are representative brightfield images of MCF-7 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
MCF-7 cell viability and growth with Nunclon Delta surfaces
- Graph (A) shows observations on mean cell viability of MCF-7 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
- Graph (B) shows mean population doubling time of MCF-7 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
MCF-7 surface marker expression with Nunclon Delta surfaces
MCF-7 cells grown on Nunclon Delta surfaces (left) or Corning TC surfaces (middle) as well as those switched to Nunclon Delta surfaces surface (right) were analyzed for the expression of endogenous receptor tyrosine protein kinase ErbB2 using flow cytometry. IgG1 was used as the isotype control. No difference was observed in the relative expression of ErbB2 using the different growth surfaces. Samples were run in duplicate on an Invitrogen Attune NxT Flow Cytometer.
Click image to enlarge
Please note: For the above MCF-7 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco MEM, 10% Gibco FBS, 1% Penicillin Streptomycin, and 0.01 mg/ml Insulin.
MCF-10A doubling times, morphology, and gene expression
MCF-10A cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
MCF-10A morphology with Nunclon Delta surfaces
Below are representative brightfield images of MCF-10A cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification
MCF-10A cell viability and growth with Nunclon Delta surfaces
- Graph (A) shows observations on mean cell viability of MCF-10A cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
- Graph (B) shows mean population doubling time of MCF-10A cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
MCF-10A gene expression with Nunclon Delta surfaces
MCF-10A cells grown on Nunclon Delta surfaces or Corning TC surfaces for at least 10 passage doublings were assayed for the expression of cell surface associated MUC1 (mucin-1) gene by qPCR. RNA was isolated using RiboPure RNA Purification Kit, cDNA was prepared using High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor, and qPCR was performed using TaqMan Fast Advanced Master Mix in a Custom TaqMan Array Plate. The normalized relative gene expression of cells grown on Corning TC surface with respect to Nunclon Delta surfaces surface is plotted on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
Please note: For the above MCF-10A Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Reconstituted Gibco Medium 171, 100 ng/ml cholera toxin, and 1% Penicillin Streptomycin.
MDA-MB-231 doubling times, morphology, and surface marker expression
MDA-MB-231 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
MDA-MB-231 morphology with Nunclon Delta surfaces
Below are representative brightfield images of MDA-MB-231 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
MDA-MB-231 cell viability and growth with Nunclon Delta surfaces
- Graph (A) shows observations on mean cell viability of MDA-MB-231 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis
- Graph (B) shows mean population doubling time of MDA-MB-231 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
MDA-MB-231 surface marker expression with Nunclon Delta surfaces
MDA-MB-231 cells grown on Nunclon Delta surfaces or Corning TC surface for 25 doublings as well as those switched to Nunclon Delta surfaces (ND) surface for 10 doublings were assessed for the expression of two endogenous surface marker proteins: ICAM-1 (top row, 0.25 μg/test) and CD44 (bottom row, 0.06 μg/test) using flow cytometry. 10,000 events were collected for each condition. No difference was observed in the surface marker expression between different growth surfaces.
Please note: For the above MDA-MB-231 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
MDCK doubling times, morphology, and gene expression
MDCK cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
MDCK morphology with Nunclon Delta surfaces
Below are representative brightfield images of MDCK cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
MDCK cell viability and growth with Nunclon Delta surfaces
- Graph (A) shows observations on mean cell viability of MDCK cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
- Graph (B) shows mean population doubling time of MDCK cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
MDCK gene expression with Nunclon Delta surfaces
As an extension to cell attachment, MDCK cells grown on Nunclon Delta surfaces or Corning TC surfaces for at least 10 passage doublings were assayed for the gene expression of endogenously expressed CDH-1 (cadherin-1), a cell adhesion marker. Cells were also assayed for expression of the characteristic endogenous gene MUC1 (mucin-1) by qPCR. RNA was isolated using RiboPure RNA purification kit, cDNA was prepared using a cDNA preparation kit, and qPCR was performed using TaqMan Assay Master Mix in a custom TaqMan array plate. The normalized relative gene expression of cells grown on Corning TC surface with respect to Nunclon Delta surfaces surface is plotted on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
Please note: For the above MDCK Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
NIH3T3 doubling times, morphology, and wound healing
NIH3T3 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
NIH3T3 morphology with Nunclon Delta surfaces
Below are representative brightfield images of NIH3T3 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
NIH3T3 cell viability and growth with Nunclon Delta surfaces
- Graph (A) shows observations on mean cell viability of NIH3T3 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
- Graph (B) shows mean population doubling time of NIH3T3 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
NIH3T3 wound healing with Nunclon Delta surfaces
- NIH3T3 cells grown on Nunclon Delta surfaces or Corning TC surfaces were assessed for cell health by their ability to undergo wound healing under serum-starved conditions on the respective surfaces.
- Quantification of images shown in A. Images were analyzed using ImageJ. The percent of wounded area over time is plotted on the y-axis. Error bars represent SEM. Data are collected from 3 separate fields for each condition. Images were captured using EVOS Cell Imaging Systems under 10X magnification.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
Please note: For the above NIH3T3 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
PC-3 doubling times, morphology, and transfection efficiency
PC-3 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.
PC-3 morphology with Nunclon Delta surfaces
Below are representative brightfield images of PC-3 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
PC-3 cell viability and growth with Nunclon Delta surfaces
- Graph (A) shows observations on mean cell viability of PC-3 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
- Graph (B) shows mean population doubling time of PC-3 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
PC-3 transfection efficiency with Nunclon Delta surfaces
PC-3 cells seeded on Nunclon Delta surfaces or Corning tissue culture (TC) treated surfaces and transfected with mCherry using Lipofectamine 3000 transfection reagent. Images of live cells were acquired 24 hours post transfection.
Please note: Flow cytometry evaluation of transfection efficiency of mCherry in PC-3 cells: 10,000 events were captured per sample. UT=Untransfected cells, T=Transfected cells. n=2.
Please note: For the above PC-3 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.
Nunclon Sphera surface performance data
HepG2 spheroid generation
HepG2 cells grown on Nunclon Sphera surface, in combination with Gibco cell culture media and fetal bovine serum have been proven to yield consistent, quality spheroids.
In a side-by-side comparison with a leading supplier, Nunclon Sphera yielded high-quality HepG2 spheroids, while the other supplier’s product yielded undesirable satellite colonies.
HepG2 cells and spheroid generation
Spheroid formation in Nunclon Sphera and Corning® ULA™ U-bottomed surface: HepG2 cells were seeded at the respective densities on Nunclon Sphera and Corning ULA U-bottomed plates for spheroid generation. On Day 6, brightfield images of spheroids were captured with EVOS imaging system using 4X objective. The figure shows representative images. Spheroids formed on the Sphera surface had no satellite colonies, while those grown on Corning ULA plates were found to have satellite colonies at higher seeding densities. Scale bar=1,000 μm.
LNCaP spheroid generation
LNCaP cells grown on Nunclon Sphera surface, in combination with Gibco cell culture media and fetal bovine serum have been proven to yield consistent, quality spheroids.
In a side-by-side comparison with a leading supplier, Nunclon Sphera yielded high-quality LNCaP spheroids, while the other supplier’s product yielded undesirable satellite colonies.
LNCaP cells and spheroid generation
Spheroid formation in Nunclon Sphera and Corning ULA U-bottomed surface: LNCaP cells were seeded at the respective densities on Nunclon Sphera and Corning ULA U-bottomed plates for spheroid generation. On Day 6, brightfield images of spheroids were captured with EVOS imaging system using 4X objective. The figure shows representative images. Spheroids formed on the Sphera surface had no satellite colonies, while those grown on Corning ULA plates were found to have satellite colonies at higher seeding densities. Scale bar=1,000 μm.
T47D spheroid generation
T47D cells grown on Nunclon Sphera surface, in combination with Gibco cell culture media and fetal bovine serum have been proven to yield consistent, quality spheroids.
In a side-by-side comparison with a leading supplier, Nunclon Sphera yielded high-quality T47D spheroids, while the other supplier’s product yielded undesirable satellite colonies.
T47D cells and spheroid generation
Spheroid formation in Nunclon Sphera and Corning ULA U-bottomed surface: T47D cells were seeded at the respective densities on Nunclon Sphera and Corning ULA U-bottomed plates for spheroid generation. On Day 6, brightfield images of spheroids were captured with EVOS imaging system using 4X objective. The figure shows representative images. Spheroids formed on the Sphera surface had no satellite colonies, while those grown on Corning ULA plates were found to have satellite colonies at higher seeding densities. Scale bar=1,000 μm.
