Conceptual art of a One Shot FBS bottle and packaging over a cell

The One Shot FBS: Fetal bovine serum in convenient 50 mL bottles

Gibco One Shot FBS is an aliquot-free solution for serum use, minimizing contamination and saving time. The One Shot FBS 50 mL bottle is designed to eliminate a tedious lab task and significantly improve your cell culture results.

Videos: Gibco One Shot FBS eliminates the need to aliquot FBS

Streamline your serum prep with One Shot FBS

Researcher quote

“This bottle allows for convenience of speed while maintaining a sterile product. As we have lots of undergrads in and out of the lab, this ensures that one mishap with not-so-aged hands doesn't ruin a whole pack of FBS.”

Bethany C. Prudner, PhD, Metabolomic Sarcoma Lab, Washington University in St. Louis

Explore the many benefits of One Shot FBS

Bottle of One Shot FBS with other FBS container types in the background

End the need to aliquot FBS

Aliquoting FBS into 50 mL conical tubes increases the chances of compromising your results. The One Shot FBS 50 mL bottle’s size and design eliminates your need to aliquot FBS, putting you in control of your time, contamination risk, and volume consistency.

Pipette drawing FBS from a One Shot FBS 50 mL bottle

Pour and pipet with ease

The bottle’s wide mouth and chamfered edge enable smooth, burp-free pouring. In addition, whether pouring or using a 25 or 50 mL pipette, you’ll be able to get every last drop.

One Shot FBS bottles placed in a rack that is soaking in a water bath

Stop the freeze-thaw-repeat cycle

Skipping the FBS aliquot step means you only have to freeze then thaw your serum once—saving considerable time and effort. Because the bottles are engineered to withstand freezing, they won’t crack and pop their lids like conical tubes often do.

Labeling on a One Shot FBS bottle versus a traditional conical tube

End labeling mishaps

Missing SKU or lot numbers, expiration dates, origin—or even the entire label—can cause a chain reaction of problems. The One Shot bottle has an easy-to-read package label, so you know exactly what you’re using.

One Shot FBS package sitting in a lab freezer next to other types of reagents

Free up freezer space

The One Shot FBS package design is optimized to ease the burden of FBS storage on your freezer capacity, improving FBS storage in your lab. In fact, the One Shot FBS 10 x 50 mL carton allows you to store up 57 L more than our 100 mL bottle.

Nine One Shot FBS bottles sitting in a blue test tube rack

Pack the rack for convenient FBS storage

Your test tube racks aren’t just for conical tubes anymore. The One Shot FBS 50 mL bottle fits in most test tube racks—including strong, durable 30 mm Nalgene Unwire Racks. So, you get the convenience of fit in an easy-pour, easy-store, aliquot-free bottle.

A variety of plastic consumables including 500 mL FBS bottle, ten conical tubes, and a plastic serological pipette

Create less waste by eliminating FBS aliquots

The One Shot FBS 50 mL bottle generates 33% less waste than aliquoting from 500 mL bottles (Table 1). By using less plastic, less petroleum feedstock is consumed, and fewer greenhouse gas emissions are generated. By choosing One Shot FBS, a typical customer using 20 L of FBS per year would gain almost 7 hours back and generate 7 lb less plastic waste. This is a significant benefit both to lab productivity and the environment.

Table 1. Waste reduction using One Shot FBS 50 mL bottles versus aliquoting from 500 mL bottle
MethodStep in procedurePlastic requiredWeight (g)Total plastic weight (g)
Aliquot from 500 mL bottleOriginal container1 x 500 mL bottle79.4 
Aliquot to conicals1 x 25 mL serological pipette18.8 
10 conical tubes140.3238.5
One Shot FBS 50 mL BottlesOriginal container10 x 50 mL bottles159.0159.0
 33% waste reduction

 

One Shot FBS bottle sitting between a bottle of Gibco DMEM and a bottle of Gibco RPMI

Pair for best performance

The 50 mL bottle size is a perfect match for use with a 500 mL bottle of Gibco RPMI, DMEM, or other Gibco cell culture media. This complete media pairing can help maximize performance and reproducibility.

For questions about the One Shot FBS 50 mL bottle's specifications, contact technical services at techsupport@thermofisher.com.

Single 50 mL bottle of One Shot FBS and a One Shot 10 x 50 mL carton

Available in single 50 mL bottle or 10-bottle pack

Choose a single 50 mL bottle or the One Shot carton, with a configuration of 10 x 50 mL.


Order One Shot FBS

One Shot FBS

Select the most cited FBS products in global scientific journals, packaged in convenient One Shot bottles.

Heat-inactivated (HI) FBS

Select Heat-Inactivated One Shot FBS, heated for 30 minutes in One Shot bottles at 56°C with mixing to inactivate complement proteins that are part of the immune response.

Learn more about our HI products at thermofisher.com/hifbs

Specialty FBS

Select Specialty One Shot FBS qualified for specific research applications and assays.

Learn more about our specialty FBS products at thermofisher.com/specialtyfbs

One Shot FBS

Select the most cited FBS products in global scientific journals, packaged in convenient One Shot bottles.

Heat-inactivated (HI) FBS

Select Heat-Inactivated One Shot FBS, heated for 30 minutes in One Shot bottles at 56°C with mixing to inactivate complement proteins that are part of the immune response.

Learn more about our HI products at thermofisher.com/hifbs

Specialty FBS

Select Specialty One Shot FBS qualified for specific research applications and assays.

Learn more about our specialty FBS products at thermofisher.com/specialtyfbs

Resources

Fetal Bovine Serum Basics
Learn the basics of FBS for cell culture, including information on the FBS uses, components, and the market dynamics driving this industry.

Cell Culture Basics
Learn the fundamentals of cell culture for achieving consistent results, including laboratory setup, safety, and aseptic techniques.

Gibco iMatch Sera Lot Matching Tool 
Provide your previous Gibco FBS lot number or answer a few questions to find your ideal serum match.

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For Research Use or Further Manufacturing Use only. Serum and blood proteins are not for direct administration into humans or animals.

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