Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Plasmid DNA (pDNA) is a fundamental component of biological research and therapeutic development for the transfer or expression of genes. When used to directly express genes, it is necessary to scale up production of high-quality, transfection-ready pDNA. GeneArt Plasmid purification services produces pDNA at a range of scales including low-endotoxin preparations suitable for early development and pre-clinical research. All purified plasmids undergo rigorous quality control and include quality assurance documentation.
An excellent option for scale-up, Thermo Fisher scientists can prepare transfection-ready, research-grade plasmid DNA (pDNA) as well as pre-clinical grade pDNA.
GeneArt Plasmid Services use proven protocols, quality-controlled systems, and reagents and GeneArt facilities are ISO9001:2015 certified. Altogether, this service has demonstrated ability in consistently providing products and services that meet customer and regulatory requirements.
The GeneArt Plasmid Purification process produces highly pure, homogenous pDNA ready for transfection and transformation. GeneArt Plasmid Services offers the following:
Table 1. Options of plasmid DNA purification scales with yields.
Please note: Plasmid purification yields of greater than 1 mg have the option of endotoxin and Bioburden testing.
Plasmid purification scale | Typical yields | Concentration | Restriction analysis | Endotoxin test | Bioburden testing |
---|---|---|---|---|---|
Midiprep (50 mL) | ~100 µg pDNA (high performing plasmids) ~10 µg pDNA (low performing plasmids) | Standard: 1 mg/mL On request: 0.1, 0.5, 2.0, 4.0 mg/mL | NA | NA | NA |
Maxiprep* (250 mL) | ~500 µg pDNA (high performing plasmids) ~50 µg pDNA (low performing plasmids) | NA | NA | NA | |
Megaprep* 1 mg guaranteed | ~1 mg pDNA (high performing plasmids) ~100 µg pDNA (low performing plasmids) | Optional | Optional | NA | |
Gigaprep* (2.5 L) | ~5mg pDNA (high performing plasmids) ~1 mg DNA (low performing plasmids) | Optional | Yes | Optional | |
2x Gigaprep* (5 L) | ~10mg pDNA (high performing plasmids) ~2 mg DNA (low performing plasmids) | Optional | Yes | Optional |
* Prepared with low endotoxin plasmid prep technology.
Plasmid DNA purification specifications and quality control include:
Applicable quality control data, based on selected scale and options, provided on the standard Certificate of Analysis (CofA).
Further documentation available upon request:
Request more information on GeneArt Services
Order support:
Geneartsupport@thermofisher.com
Contact us:
North America and Asia: 1-800-955-6288
Europe: +49 (0)941 942 76-100
For Research Use Only. Not for use in diagnostic procedures.