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ReadyPouch™ Agarose Gels—DEACTIVATING THIS ENTIRE PAGE
 | Just vent, heat, add stain, and pourThere is nothing worse than carefully weighed agarose, gently swirled in buffer, that turns into a scalding, molten mess in the microwave. That’s where ReadyPouch™ Agarose Gels come in. ReadyPouch™ Agarose Gels are new multipurpose UltraPure™ agarose gels for virtually every DNA electrophoresis application. Premixed with 1X TAE buffer, ReadyPouch™ Agarose Gels are the simple-to-use 1% agarose gel solution. |
ReadyPouch agarose video - 4 simple steps
Traditional agarose: A tale of too many steps
What researchers are saying
"I don't know why anyone would want to make their gel from scratch."
"One less step. Just grab-n-go."
ReadyPouch™ Agarose Gel benefits
- No boil-overs
- Wide resolution range
- High gel strength
- Ready-to-use agarose (1%) premixed in TAE
- Fewer bubbles
- No detectable DNase or RNase activity
ReadyPouch™ Agarose Gel applications
- PCR fragments >0.5 kb
- Southern blotting
- Analytical separation of DNA fragments in 0.5–23 kb range
ReadyPouch™ Agarose Gels—4 easy steps
1
Vent
VENT by tearing pouch along the dotted line
2
Heat
HEAT in microwave on HIGH for 90 seconds
3
Add
ADD 2 drops of stain
4
Pour
POUR into casting tray
Comparison of gel made with ReadyPouch™ Agarose Gel to a standard 1% agarose gel
A 1% Agarose Gel
B ReadyPouch™ Agarose Gel
Lambda DNA was digested with various restriction enzymes for 1 hour, and 250 ng of each digest was then loaded on a standard 1% agarose gel (A) and a ReadyPouch™ Agarose Gel (B). The gels were run for 30 min at 85 V and imaged on an E-Gel® Imager for 0.5 sec. Lanes 1 and 14: 250 ng of 1 Kb Plus DNA Ladder. Lane 2: 2 µL Low Mass DNA Ladder. Lanes 3 and 4: AgeI digest. Lanes 5 and 6: AseI digest. Lanes 7 and 8: BamHI digest. Lanes 9 and 10: PvuII digest. Lanes 11 and 12: HindIII digest. Lane 13: BamHI/HindIII double digest.
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