UltraPure Agarose and Reagents

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Enjoy the benefits of a quality product in easy-to-use packaging. Our reagent pouches feature a spout that make pouring and dispensing the powder easier, reducing the likelihood of spills and contamination.

UltraPure Agarose is standard melting-point agarose designed for routine separation analysis of DNA and RNA fragments in the 500–23,000 bp range. UltraPure Agarose 1000 is a specialized agarose that provides higher resolution of PCR fragments and other short DNA fragments. Agarose 1000 is easier to handle because it has a stronger gel structure: gel strength >1,400 g/cm2. For low melting/gelling temperature agarose, UltraPure Low Melting Point Agarose is recommended for rapid DNA gel extraction protocols. Listed below are some reasons and tips to use LMP agarose.

Low melting point (LMP) agarose is ideal for resolving DNA fragments from 10 bp to 1,000 bp. The major advantage of LMP agarose is the low melting temperature of 65°C or less. At these temperatures, it is possible to remelt the agarose of a gel without melting the double helix of DNA molecules in the agarose. Therefore, slices of LMP agarose containing DNA can be melted and the nucleic acids manipulated directly in the remelted agarose.

Applications and references for LMP agarose:

1. In-gel ligation and transformation: Struhl K (1985) A rapid method for creating recombinant DNA molecules. BioTechniques 3:452–453.
2. In-gel restriction digestion: Parker RC, Seed B (1980) Two-dimensional agarose gel electrophoresis “SeaPlaque” agarose dimension. Methods Enzymol 65(1):358–363.
3. In-gel PCR: Bjourson AJ, Cooper JE (1992) Band-stab PCR: a simple technique for the purification of individual PCR products. Nucleic Acids Res 20(17):4675.
4. Cycle sequencing: Gorman KB, Steinberg RA (1989) Simplified method for selective amplification and direct sequencing of cDNAs. Biotechniques 7(4):326–328, 331.
5. Blunt-end ligation: Hemsley A, Arnheim N, Toney MD, et al. (1989) A simple method for site-directed mutagenesis using the polymerase chain reaction. Nucleic Acids Res 17(16):6545–6551.
6. DNA recovery with β-Agarase: Crouse J and Amorese D (1987) Ethanol precipitation: ammonium acetate as an alternative to sodium acetate. GIBCO-BRL Focus 9:3–5.
7. DNA recovery with phenol/chloroform: Benson SA (1984) A rapid procedure for isolation of DNA fragments from agarose gels. BioTechniques 2: 66–67.

Tips for using LMP agarose:

• Use 1x TAE buffer instead of 1x TBE buffer
• Stain the gel after electrophoresis with SYBR Safe DNA gel stain. Ethidium bromide staining can result in DNA degradation.
• Visualize DNA with UV light source ≥300 nm and minimize exposure to less than 1 minute.
• Melt the gel slice at 65°C. Do not exceed 70°C, which may result in DNA denaturation.

To make it easier to find your reagent of choice, we have grouped our UltraPure reagents by application and common reagents.

Agarose and polyacrylamide gel electrophoresis

Southern blot analysis

Enzymes

Northern blot analysis

Phenol products

Common reagents

For Research Use Only. Not for use in diagnostic procedures.