Oligo(dT) and Random DNA Primers | Thermo Fisher Scientific

In addition to our custom synthesis options for oligos, primers, probes, and genes, Thermo Fisher Scientific offers a selection of other high-quality, pre-designed DNA primers and oligos. These products include random primers and oligo (dT) primers for first strand cDNA synthesis, gene specific primers for sequencing, forward and reverse sequencing primers and kits, and the MegaPlex RT Primers and Megaplex primer pools developed for assessing expression profiles.

Review our pre-designed, pre-synthesized offerings that are commonly used in a variety of molecular biology and genomic applications ranging from viral detection, construction of cDNA libraries, expression analyses, RNAi-based studies, epigenetic research, next generation sequencing, and more.

Random DNA primers and hexamers

Random primers and hexamers are short oligodeoxyribonucleotides with random base sequences (usually [d(N)6]), typically used to prime mRNAs with or without poly(A) for cDNA synthesis. Unlike oligo(dT) primers, these primers are not template specific and are suitable for DNA synthesis using Klenow fragments with DNA templates or for cDNA synthesis using reverse transcriptase with mRNA templates.

Learn more about their application in reverse transcription reactions

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Oligo (dT) primers

Typically, oligo (dT) primers are a string of 12–20 deoxythymidines that specifically anneal to poly(A) tails of eukaryotic mRNAs. In contrast to random primers, oligo (dT) primers are not suitable for degraded samples but are excellent for constructing cDNA libraries and recommended for use with reverse transcriptases (RTs) as Superscript IV RT.

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Sequencing primers

Common sequences of Short DNA oligonucleotides are often used as primers for sequencing cloned genes (Sanger sequencing). Choose from our commonly used sequencing primers to initiate your sequence verification projects.

Learn about sequencing applications

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Primer pools

Different colors portraying different primer pool assays

Pools of pre-designed primers can help generate comprehensive expression profiles, especially where assay sensitivity is critically important. The collection of MegaPlex RT Primers and Megaplex Primer Pools significantly enhance the ability to detect microRNAs expressed at low levels with as low as 1 ng of input total RNA.

Read how Megaplex Primer Pools were used to profile miRNAs with limited samples