Our comprehensive 2′ modified RNA phosphoramidites portfolio is uniquely designed for oligonucleotide therapeutic development and manufacturing. Seamlessly scale up your synthesis of siRNA and ASO for RNA therapeutics from early-stage development to commercialization with the comprehensive supply of Thermo Scientifics phosphorarmidites. 

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Thermo Scientific 2′ modified RNA phosphoramidites

Modifications at the 2′ position of the ribose ring are commonly used to help increase oligonucleotide stability and improve resistance to nuclease activity in vivo. Our 2'-modified RNA phosphoramidites are manufactured to precise standards to help ensure quality, consistency, and reliability for efficient synthesis of nucleic acid-based therapeutics. Multiple quality grades of TheraPure, standard, or fast deprotect phosphoramidites are available to choose from.


2'-OMe phosphoramidites

TheraPure and standard grades 2′-OMe phosphoramidites are available for oligo synthesis of diagnostics or therapeutics manufacturing. For faster deprotection times, consider reviewing the PAC chemistries in combination with 2'-OMe U (no base protection) and Ac-rC. 

Available 2′-OMe modified RNA phosphoramidites by synthesizer compatibility

Modification

2'-OMe phosphoramidites

Common applicationssiRNA (small interfering RNA)Diagnostics

Quality grades

Fast deprotect

Featuring quality attribute

  • TheraPure: Maximal QC and analytical testing supported by documentation
  • Standard: Highly reliable for routine oligo synthesis
Allow the removal of base-protecting groups under mild or accelerated conditions 
 Bz-AUGCPAC-AiPrPAC-G
Applied Biosystems/AKTA Synthesizer27-2042-0427-2044-0427-2046-0427-2043-0427-1822-0227-1826-02
Expedite
Synthesizer		27-2042-1027-2044-1027-2046-1027-2043-1027-1822-1027-1826-10
MerMade
Synthesizer

Custom orders are available.
Request quote

27-1822-1127-1826-11


Manufacturing for therapeutics with high purity requirement

Quality attributes of 2′-OMe phosphoramidites are presented in the table below.

  Click images to enlarge

 2′-OMe-Bz-A-CEP2′-OMe-U-CEP2′-OMe-iBu-G-CEP2′-OMe-Ac-C-CEP
TheraPure grade Cat. No.27-204227-204427-204627-2043
Standard grade Cat. No.27-184227-182527-184627-1823
FormulaC48H54N7O8PC40H56N7O9PC45H56N7O9PC42H52N5O9P
Molecular weight887.97 g/mol760.8 g/mol869.9 g/mol801.86 g/mol
CAS #110782-31-5110764-79-9150780-67-9199593-09-4
AppearanceColor intensity must not exceed Pantone #P1-4U or #P4-2U
HPLC purity (TheraPure phosphoramidites)≥ 98.0% | (>99%) *
M-11 Impurity≤ 0.01%
31P NMR purity (TheraPure phosphoramidites)≥ 98.0% | (>99%) *
Shipping / Storage  conditionAmbient / –20 °C
Water content ≤ 0.2% (KF Method)
Material OriginNon-animal source (no TSE/BSE risk)

Compatible products by synthesizer model

Available 2′-OMe phosphoramidites in TheraPure or standard grades and are compatible with the following oligonucleotide synthesizers: 

  • Applied Biosystems / AKTA Synthesizer
  • Expedite Synthesizer
  • MerMade Synthesizer—Custom order only; contact us for a quote
Compatible products with the Applied Biosystems and AKTA synthesizer

Compatible products are in amber bottles with serum vial neck style as ready-to-ship catalog SKU for your siRNA and ASO synthesis.

Expedite synthesizer

Available catalog SKUs are supplied in 30 mL amber bottle with a 20-400 finish neck style. Compatible with the Expedite synthesizer for antisense, siRNA, aptamer, or gRNA-based research. 

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Manufacturing for diagnostic applications

Utilize PAC-A and iPrPAC-G with Ac-C and U to minimize deprotection times. Acetylated amidites (such as Ac-G, SY5400) are excellent for shortening deprotection times for longmers.

  Click images to enlarge

 2′-OMe-PAC-A-CEP2′-OMe-iPrPAC-G-CEPTheraPure 2'-OMe-Ac-G-CEP
Cat. No.27-182227-1826SY5400
FormulaC49H56N7O9PC52H62N7O10PC43H52N7O9P
Molecular weight918.0 g/mol976.1 g/mol841.9 g/mol
CAS #128219-82-9NA909033-40-5
HPLC purity≥ 98.0%≥ 98.0%≥ 99%
31P NMR purity≥ 98.0%≥ 98.0%≥ 99%
HazardousNo
Shipping / Storage  conditionAmbient / –20 °C
Material OriginNon-animal source (no TSE/BSE risk)
C52H62N7O10P
Compatibility by synthesizer model

2′-OMe modified RNA phosphoramidites are compatible with the following oligonucleotide synthesizers: 

  • Applied Biosystems and AKTA Synthesizer
  • Expedite Synthesizer
  • MerMade Synthesizer
Applied Biosystems and AKTA synthesizer

Compatible catalog products are ready-to-ship in amber bottles with serum vial neck style.

Expedite synthesizer

Available catalog SKUs are supplied in 30 mL amber bottle with a 20-400 finish neck style. 

MerMade systems synthesizer

Compatible products are available to ship in a 30 mL amber bottle with a 28-400 finish neck style.

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2'-Fluoro Phosphoramidites

Synthesizing RNA oligonucleotides using 2′-Fluoro Bz A, iBu G, Ac C, and U phosphoramidites increases the thermal stability of RNA duplexes and increases the nuclease resistance of single-stranded RNA. These phosphoramidites can be incorporated into custom siRNA oligonucleotides for use in gene-targeting and suppression studies.

Available 2'-Flouro modified phosphoramidites by compatible synthesizers

Modifications2'-Fluoro phosphoramidites
Common applicationssiRNA (small interfering RNA)
Quality gradesTheraPureStandard
Featuring quality attributeMaximal QC and analytical testing supported by documentationTraditional base protection method. Highly reliable for routine oligo synthesis
 Ac-CUAc-GBz-AiBu-G

Applied Biosystems/AKTA Synthesizer

27-1604-0227-1602-02

Custom orders are available.

Request quote

 

27-1601.0227-1607-02
Expedite
Synthesizer		27-1604-0927-1602-0927-1601-0927-1607-09
MerMade
Synthesizer		27-1604-12

27-1602-12

 

27-1601.1227-1607-12


Technical specifications for 2'-Flouro modification

  Click images to enlarge

 2′-F-Ac-C-CEP2′-F-U-CEP2'-F-Ac-G-CEP2′-F-Bz-A-CEP2′-F-iBu-G-CEP
Quality gradeTherapureStandard
Cat. No.27-160427-1602SY5410*27-160127-1607
FormulaC41H49N5FO8PC39H46FN4O8PC42H49FN7O8PC47H51FN7O7PC44H53FN7O8P
Molecular weight798.8 g/mol748.8 g/mol829.87 g/mol875.92 g/mol857.4 g/mol
CAS #159414-99-0146954-75-8N/AN/A144089-97-4
Residual Solvent≤ 3.0% total % by weight solventN/A
AppearanceWhite to light yellow powder
HPLC purity*>98%
31P NMR purity*>98%
Solution ClarityFree from undissolved particulate at 0.2 M solution in ACN
Shipping / Storage conditionAmbient / –20 C
Water content≤ 0.3%
Material OriginNon-animal source (no TSE/BSE risk)


Compatibility by synthesizer model

2′-Fluoro modified RNA phosphoramidites are compatible with the following oligonucleotide synthesizers: 

  • Applied Biosystems / AKTA Synthesizer
  • Expedite Synthesizer
  • MerMade Synthesizer

Applied Biosystems and AKTA synthesizer

Order compatible products in amber bottles with serum vial neck style. Available in TheraPure and standard quality grades.

Expedite synthesizer

Available catalog SKUs are supplied in 30 mL amber bottle with a 20-400 finish neck style. Choose from quality grades of TheraPure or standard.

MerMade systems synthesizer

Catalog SKUs available that are compatible with the MerMade synthesizer in a 30 mL amber bottle with a 28-400 finish neck style.

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2'-OME phosphoramidites

RNA oligonucleotides synthesized using 2′-MOE modifications phosphoramidites have shown to be more nuclease resistant, with lower toxicity, and slightly increased hybridization affinities, making them well suited for therapeutic in vivo applications, such as antisense (ASO), siRNA, and aptamers. 

  • Tightly controlled, critical impurities (e.g., M-11)
  • Suitable for use with DNA and/or RNA phosphoramidites
  • Reliable, high quality oligonucleotide synthesis

Available 2'-OME modified phosphoramidites by compatible synthesizers

Modifications2'-MOE phosphoramidites
Common applicationsASO (antisense oligonucleotides)
Quality gradesTheraPure
Featuring quality attributeMaximal QC testing to help ensure impurities and residual solvents are within the stringent requirement for oligo therapeutics
 A5mCGT
Applied Biosystems/AKTA Synthesizer27-1019-0227-1020-0227-1022-0227-1021-02
Expedite
Synthesizer

Custom orders are available.
Request quote

MerMade
Synthesizer		27-1019-1127-1020-1127-1022-1127-1021-11


Technical specifications of 2'-OME modification

  Click images to enlarge

 2′-MOE-A CEP2′-MOE-5mC CEP2′-MOE-G CEP2′-MOE-T CEP
Quality gradeTheraPureTheraPureTheraPureTheraPure
Cat. No.27-101927-102027-102227-1021
FormulaC50H58N7O9PC50H60N5O10PC47H60N7O10PC43H55N4O10P
Molecular weight932.03 g/mol922.0 g/mol914.01 g/mol819.0 g/mol
CAS #251647-53-7163759-94-2251647-55-9163878-63-5
Solution clarityFree from undissolved particulate at 0.2 M solution in ACN
HPLC purity*

 ≥99%

31P NMR purity*≥99%
Shipping / Storage  conditionAmbient / –20°C
Water content≤0.3% (by KF Method)
Total residual solvents≤4.0%
Material originNon-animal source (no TSE/BSE risk)

Compatibility by synthesizer model

TheraPure RNA phosphoramidites with 2′-MOE modifications are compatible with the following oligonucleotide synthesizers: 

  • Applied Biosystems / AKTA Synthesizer
  • Expedite Synthesizer: Custom order only; contact us for a quote
  • MerMade Synthesizer
Applied Biosystems and AKTA synthesizer

Compatible catalog products of 1 g or 5 g are ready-to-ship in amber bottles with serum vial neck style. 

MerMade systems synthesizer

Catalog SKUs compatible to MerMade synthesizer are ready-to-ship in 30 mL amber bottle with a 28-400 finish neck style. Sizing options of 1 g or 2 g are available to choose from.

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Resources

Phosphoramidite Learning Resource

Access webinars, videos, articles, and more to optimize your success in oligonucleotide synthesis for diagnostic and therapeutic manufacturing process development.

mRNA Production Learning Library

Browse the educational assets related to raw materials selection, process development using in vitro transcription, scale-up synthesis, and the latest trends in mRNA production.

Stylesheet for Classic Wide Template adjustments

For Research Use Only. Not for use in diagnostic procedures.