82036-qIP-Magnetic-Myc-Kit-230

Our qIP Protein Interaction Kits use anti-HA or anti-Myc beads (agarose or magnetic) and a sensitive luciferase assay system to co-immunoprecipitate (co-IP) and quantify interactions between epitope-tagged and TurboLuc luciferase enzyme (Tluc) tagged protein pairs expressed in mammalian cells. The quantitative immunoprecipitation (qIP) system uses Tluc enzyme to accurately and precisely reflect the abundance of a specific co-IP product, to help avoid the need for time-consuming gel electrophoresis, western blotting, and band densitometry.

Learn more about

Featured qIP products

An agarose bead format of our qIP kit, using HA tagging

Exploit the convenience of magnetic bead-mediated qIP

Recover and quantify proteins that bind to your Myc-tagged bait protein

Magnetic beads make quantitative immunoprecipitation fast and robust

Features of qIP kits

  • Quantitative—integrated luciferase assay enables direct measurement of co-IP products, and control system helps ensure accuracy and normalization
  • Sensitive—bright bioluminescence signal allows detection of weak interactions
  • Time-saving—final read-out is relative luminescence units (RLU), rather than  elution and western blotting
  • Simple and fast—incubation and wash steps use spin columns or microcentrifuge tubes
  • Robust—vectors, kit, and qIP method is compatible with various  mammalian cell lines (293T, HEK293, NIH3T3, and CHO-K1)
  • Versatile—highly customizable assay platform :
    • Additional cloning vectors are available for N- and C-terminal fusions
    • HA- and c-Myc tag kits are available in both agarose and magnetic bead formats
    • Assay reagents and buffers are available separately 

Schematic of the qIP assay procedure

qIP-002-Generic-Protocol

For Research Use Only. Not for use in diagnostic procedures.