Protein Extraction for Mass Spectrometry

Cell lysis is the first step when extracting proteins for mass spectrometry applications. Fractionation may be required to enrich for proteins of interest or to study protein localization. The kits featured here are convenient, easy to use, and have been validated for downstream analysis with Thermo Scientific mass spectrometers. They include options for either total protein extraction or isolation of proteins from specific cellular fractions. The Thermo Scientific EasyPep Mini MS Sample Prep Kit provides pre-formulated reagents and a robust method for the preparation of high quality samples that are ready for MS analysis in less than 3 hours.

• Optimized—extract total protein or specific cellular fractions
• Effective—enable high protein yields with low cross-contamination for fractionation kits
• Validated—extract from cultured mammalian cells have been successfully analyzed using Thermo Scientific mass spectrometers
• Convenient—minimize the need for mechanical cell disruption
   

Choose the right protein extraction kit for your mass spec workflow

	Order now	Order now	Order now
	Thermo Scientific EasyPep Mini MS Sample Prep Kit	Mem-PER Plus Membrane Protein Extraction Kit	Subcellular Protein Fractionation Kit for Cultured Cells
Fractions isolated	Primarily cytosolic	Integral and membrane-associated proteins	Nucleus, cytoplasm, membrane, cytoskeletal, chromatin-bound
Amount of sample processed	20 samples of 10-100 µg of cell pellet or tissue	50 samples of 5 million cells each	50 samples of 2 million cells each (20 µL packed)
Sample processing time (pre-digestion)	<30 min	1 hr	2–3 hr
Digestion proteases included?	Yes	No	No
Total sample preparation time	1.5-3.5 hr	24-48 hr	24-48 hr

Representative quantitation of protein enrichment of marker proteins (Hsp90 and histone 3) using TMTsixplex reagents in different subcellular fractions relative to whole cell lysate. The red bars show the relative intensity of TMT reagent reporter ions for unique marker proteins. Subcellular fractions were prepared using the Thermo Scientific Subcellular Protein Fractionation Kit for Cultured Cells (Cat. No. 78840).

Identification of peptides by LC-MS/MS from immunoprecipitated Na⁺/ K⁺-ATPase transport complex extracted with the Mem-PER Plus Kit (Cat. No. 89842). Samples were processed by in-solution digestion according to the kit protocol, and analyzed on the Orbitrap Fusion mass spectrometer.